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TitleB. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage.
Journal, issue, pagesEMBO J, Vol. 43, Issue 4, Page 484-506, Year 2024
Publish dateDec 14, 2023
AuthorsEsther N Park / Timur Mackens-Kiani / Rebekah Berhane / Hanna Esser / Chimeg Erdenebat / A Maxwell Burroughs / Otto Berninghausen / L Aravind / Roland Beckmann / Rachel Green / Allen R Buskirk /
PubMed AbstractStalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli, these pathways are triggered by ribosome collisions through the ...Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli, these pathways are triggered by ribosome collisions through the recruitment of SmrB, a nuclease that cleaves the mRNA. In B. subtilis, the related protein MutS2 was recently implicated in ribosome rescue. Here we show that MutS2 is recruited to collisions by its SMR and KOW domains, and we reveal the interaction of these domains with collided ribosomes by cryo-EM. Using a combination of in vivo and in vitro approaches, we show that MutS2 uses its ABC ATPase activity to split ribosomes, targeting the nascent peptide for degradation through the ribosome quality control pathway. However, unlike SmrB, which cleaves mRNA in E. coli, we see no evidence that MutS2 mediates mRNA cleavage or promotes ribosome rescue by tmRNA. These findings clarify the biochemical and cellular roles of MutS2 in ribosome rescue in B. subtilis and raise questions about how these pathways function differently in diverse bacteria.
External linksEMBO J / PubMed:38177497 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 6.8 Å
Structure data

EMDB-18558, PDB-8qpp:
Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-18585: Cryo-EM reconstruction of Bacillus subtilis collided disome binding MutS2 SMR and KOW domains
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-18586: Cryo-EM reconstruction of crosslinked native Bacillus subtilis collided disome binding MutS2 SMR and KOW domains
Method: EM (single particle) / Resolution: 6.8 Å

EMDB-18901: Bacillus subtilis MutS2-collided disome complex (stalled 70S)
PDB-8r55: Bacillus subtilis MutS2-collided disome complex (collided 70S)
Method: EM (single particle) / Resolution: 3.57 Å

Source
  • bacillus subtilis (bacteria)
KeywordsRIBOSOME / Muts2 / collision / disome / splitting / quality control

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