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- EMDB-18558: Bacillus subtilis MutS2-collided disome complex (stalled 70S) -

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Basic information

Entry
Database: EMDB / ID: EMD-18558
TitleBacillus subtilis MutS2-collided disome complex (stalled 70S)
Map dataFocus refined map filtered at FSC resolution
Sample
  • Complex: MutS2-disome complex from B. subtilis, focus refined, stalled 70S
    • Protein or peptide: x 48 types
    • RNA: x 5 types
KeywordsMuts2 / collision / disome / splitting / quality control / RIBOSOME
Function / homology
Function and homology information


mismatched DNA binding / negative regulation of DNA recombination / ATP-dependent DNA damage sensor activity / mismatch repair / large ribosomal subunit / small ribosomal subunit / transferase activity / endonuclease activity / tRNA binding / Hydrolases; Acting on ester bonds ...mismatched DNA binding / negative regulation of DNA recombination / ATP-dependent DNA damage sensor activity / mismatch repair / large ribosomal subunit / small ribosomal subunit / transferase activity / endonuclease activity / tRNA binding / Hydrolases; Acting on ester bonds / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / ATP hydrolysis activity / zinc ion binding / ATP binding / metal ion binding / cytoplasm
Similarity search - Function
MutS2 and Smr-associated SH3 domain / MutS2 and Smr-associated SH3 domain / Endonuclease MutS2 / Smr domain superfamily / Smr domain / Small MutS-related domain / Smr domain / Smr domain profile. / DNA mismatch repair MutS family / DNA mismatch repair protein MutS, C-terminal ...MutS2 and Smr-associated SH3 domain / MutS2 and Smr-associated SH3 domain / Endonuclease MutS2 / Smr domain superfamily / Smr domain / Small MutS-related domain / Smr domain / Smr domain profile. / DNA mismatch repair MutS family / DNA mismatch repair protein MutS, C-terminal / DNA mismatch repair protein MutS, core / DNA mismatch repair protein MutS, core domain superfamily / MutS domain V / DNA mismatch repair proteins mutS family signature. / DNA-binding domain of DNA mismatch repair MUTS family / ATPase domain of DNA mismatch repair MUTS family / Type-1 KH domain profile. / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L17 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site
Similarity search - Domain/homology
Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL17 ...Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL6 / Endonuclease MutS2 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL29
Similarity search - Component
Biological speciesBacillus subtilis (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsPark E / Mackens-Kiani T / Berhane R / Esser H / Erdenebat C / Burroughs AM / Berninghausen O / Aravind L / Beckmann R / Green R / Buskirk AR
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research Foundation (DFG)TRR174 Germany
Citation
Journal: EMBO J / Year: 2024
Title: B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage.
Authors: Esther N Park / Timur Mackens-Kiani / Rebekah Berhane / Hanna Esser / Chimeg Erdenebat / A Maxwell Burroughs / Otto Berninghausen / L Aravind / Roland Beckmann / Rachel Green / Allen R Buskirk /
Abstract: Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli, these pathways are triggered by ribosome collisions through the ...Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli, these pathways are triggered by ribosome collisions through the recruitment of SmrB, a nuclease that cleaves the mRNA. In B. subtilis, the related protein MutS2 was recently implicated in ribosome rescue. Here we show that MutS2 is recruited to collisions by its SMR and KOW domains, and we reveal the interaction of these domains with collided ribosomes by cryo-EM. Using a combination of in vivo and in vitro approaches, we show that MutS2 uses its ABC ATPase activity to split ribosomes, targeting the nascent peptide for degradation through the ribosome quality control pathway. However, unlike SmrB, which cleaves mRNA in E. coli, we see no evidence that MutS2 mediates mRNA cleavage or promotes ribosome rescue by tmRNA. These findings clarify the biochemical and cellular roles of MutS2 in ribosome rescue in B. subtilis and raise questions about how these pathways function differently in diverse bacteria.
#1: Journal: Biorxiv / Year: 2023
Title: An evolutionarily conserved strategy for ribosome binding and inhibition by beta-coronavirus non-structural protein 1
Authors: Maurina SF / O'Sullivan JP / Sharma G / Rodriguez DCP / MacFadden A / Cendali F / Henen MA / Kieft JS / Glasgow A / Steckelberg A
History
DepositionOct 2, 2023-
Header (metadata) releaseDec 27, 2023-
Map releaseDec 27, 2023-
UpdateFeb 28, 2024-
Current statusFeb 28, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_18558.png

Downloads & links

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Map

FileDownload / File: emd_18558.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFocus refined map filtered at FSC resolution
Voxel sizeX=Y=Z: 1.045 Å
Density
Contour LevelBy AUTHOR: 0.377
Minimum - Maximum-0.7371306 - 1.9228493
Average (Standard dev.)0.014376461 (±0.10599903)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 522.5 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_18558_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_18558_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : MutS2-disome complex from B. subtilis, focus refined, stalled 70S

EntireName: MutS2-disome complex from B. subtilis, focus refined, stalled 70S
Components
  • Complex: MutS2-disome complex from B. subtilis, focus refined, stalled 70S
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: Large ribosomal subunit protein bL33
    • Protein or peptide: Large ribosomal subunit protein bL34
    • Protein or peptide: Large ribosomal subunit protein bL35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: Large ribosomal subunit protein bL31
    • Protein or peptide: 50S ribosomal protein L9
    • RNA: 16S rRNA (1533-MER)
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: tRNA (77-MER)
    • RNA: mRNA (33-MER)
    • Protein or peptide: Endonuclease MutS2
    • RNA: 5S rRNA (112-MER)
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein uL5
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein bL17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: Large ribosomal subunit protein bL21
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • RNA: 23S ribosomal RNA

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Supramolecule #1: MutS2-disome complex from B. subtilis, focus refined, stalled 70S

SupramoleculeName: MutS2-disome complex from B. subtilis, focus refined, stalled 70S
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#6, #51, #53, #7-#50, #52
Source (natural)Organism: Bacillus subtilis (bacteria)

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Macromolecule #1: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 6.745073 KDa
SequenceString:
MAVPFRRTSK MKKRLRRTHF KLNVPGMTEC PSCGEMKLSH RVCKACGSYN GKDINVKSN

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #2: Large ribosomal subunit protein bL33

MacromoleculeName: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 5.786696 KDa
SequenceString:
MRVNITLACT ECGERNYISK KNKRNNPDRV EFKKYCPRDK KSTLHRET

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #3: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 5.271332 KDa
SequenceString:
MKRTFQPNNR KRSKVHGFRS RMSSKNGRLV LARRRRKGRK VLSA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #4: Large ribosomal subunit protein bL35

MacromoleculeName: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 7.597128 KDa
SequenceString:
MPKMKTHRGS AKRFKKTGSG KLKRSHAYTS HLFANKSQKQ KRKLRKSSVV SAGDFKRIKQ MLANIK

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #5: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIRRK GKVMVICENP KHKQKQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #6: Large ribosomal subunit protein bL31

MacromoleculeName: Large ribosomal subunit protein bL31 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 7.216244 KDa
SequenceString:
MKAGIHPNFK KATVKCACGN EFETGSVKEE VRVEICSECH PFYTGRQKFA SADGRVDRFN KKYG

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #7: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 28.009297 KDa
SequenceString: MSVISMKQLL EAGVHFGHQT RRWNPKMKRY IFTERNGIYI IDLQKTVKKV EEAYNFTKNL AAEGGKILFV GTKKQAQDSV KEEAQRSGM YYVNQRWLGG TLTNFETIQK RIKRLKDIEK MQENGTFDVL PKKEVVQLKK ELERLEKFLG GIKDMKDLPD A LFIIDPRK ...String:
MSVISMKQLL EAGVHFGHQT RRWNPKMKRY IFTERNGIYI IDLQKTVKKV EEAYNFTKNL AAEGGKILFV GTKKQAQDSV KEEAQRSGM YYVNQRWLGG TLTNFETIQK RIKRLKDIEK MQENGTFDVL PKKEVVQLKK ELERLEKFLG GIKDMKDLPD A LFIIDPRK ERIAVAEARK LNIPIIGIVD TNCDPDEIDV VIPANDDAIR AVKLLTSKMA DAILEAKQGE EEAEVAEETA PE TETTTA

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #8: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 24.351889 KDa
SequenceString: MGQKVNPVGL RIGVIRDWES KWYAGKDYAD FLHEDLKIRE YISKRLSDAS VSKVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKA LNSLTGKRVH INILEIKRAD LDAQLVADNI ARQLENRVSF RRAQKQQIQR TMRAGAQGVK TMVSGRLGGA D IARSEYYS ...String:
MGQKVNPVGL RIGVIRDWES KWYAGKDYAD FLHEDLKIRE YISKRLSDAS VSKVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKA LNSLTGKRVH INILEIKRAD LDAQLVADNI ARQLENRVSF RRAQKQQIQR TMRAGAQGVK TMVSGRLGGA D IARSEYYS EGTVPLHTLR ADIDYATSEA DTTYGKLGVK VWIYRGEVLP TKKKTEEGGK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #9: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 22.874271 KDa
SequenceString: MARYTGPSWK LSRRLGISLS GTGKELEKRP YAPGPHGPGQ RKKLSEYGLQ LQEKQKLRHM YGVNERQFRT LFDKAGKLAG KHGENFMIL LDSRLDNVVY KLGLARTRRQ ARQLVNHGHI LVDGSRVDIP SYLVKPGQTI GVREKSRNLS IIKESVEVNN F VPEYLTFD ...String:
MARYTGPSWK LSRRLGISLS GTGKELEKRP YAPGPHGPGQ RKKLSEYGLQ LQEKQKLRHM YGVNERQFRT LFDKAGKLAG KHGENFMIL LDSRLDNVVY KLGLARTRRQ ARQLVNHGHI LVDGSRVDIP SYLVKPGQTI GVREKSRNLS IIKESVEVNN F VPEYLTFD AEKLEGTFTR LPERSELAPE INEALIVEFY SR

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #10: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 17.650625 KDa
SequenceString:
MRRIDPSKLE LEERLVTVNR VAKVVKGGRR FRFAALVVVG DKNGHVGFGT GKAQEVPEAI RKAVEDAKKN LIEVPMVGTT IPHEIIGRF GAGNILLKPA SEGTGVIAGG PVRAVLELAG VADILSKSLG SNTPINMIRA TLQGLSELKR AEDVAKLRGK S VEELLG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #11: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 11.140548 KDa
SequenceString:
MRKYEVMYII RPNIDEESKK AVIERFNNVL TSNGAEITGT KDWGKRRLAY EINDFRDGFY QIVNVQSDAA AVQEFDRLAK ISDDIIRHI VVKEEE

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #12: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 17.915879 KDa
SequenceString:
MPRKGPVAKR DVLPDPIYNS KLVSRLINKM MIDGKKGKSQ TILYKSFDII KERTGNDAME VFEQALKNIM PVLEVKARRV GGANYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEER LANEILDAAN NTGAAVKKRE DTHKMAEANK AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #13: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 14.901427 KDa
SequenceString:
MVMTDPIADM LTRIRNANMV RHEKLEIPAS KLKREIAEIL KREGFIRDVE FVEDSKQGII RVFLKYGQNN ERVITGLKRI SKPGLRVYA KSNEVPRVLN GLGIAIISTS QGVLTDKEAR AKQAGGEVLA YVW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #14: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 14.335504 KDa
SequenceString:
MAQVQYYGTG RRKSSVARVR LVPGEGRIVV NNREISEHIP SAALIEDIKQ PLTLTETAGT YDVLVNVHGG GLSGQAGAIR HGIARALLE ADPEYRTTLK RAGLLTRDAR MKERKKYGLK GARRAPQFSK R

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #15: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 11.687661 KDa
SequenceString:
MAKQKIRIRL KAYDHRILDQ SAEKIVETAK RSGASVSGPI PLPTEKSVYT ILRAVHKYKD SREQFEMRTH KRLIDIVNPT PQTVDALMR LDLPSGVDIE IKL

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #16: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 13.993051 KDa
SequenceString:
MAAARKQNTR KRRVKKNIES GIAHIRSTFN NTIVTITDTH GNAISWSSAG ALGFRGSRKS TPFAAQMAAE TAAKGSIEHG LKTLEVTVK GPGSGREAAI RALQAAGLEV TAIRDVTPVP HNGCRPPKRR RV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #17: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 15.248736 KDa
SequenceString:
MPTINQLIRK GRVSKVENSK SPALNKGYNS FKKEHTNVSS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL TNGIEVTAYI PGIGHNLQE HSVVLIRGGR VKDLPGVRYH IVRGALDTAG VENRAQGRSK YGTKKPKAK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #18: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 13.818085 KDa
SequenceString:
MARIAGVDIP RDKRVVISLT YIFGIGRTTA QQVLKEAGVS EDTRVRDLTE EELGKIRDII DKLKVEGDLR REVSLNIKRL IEIGSYRGI RHRRGLPVRG QNSKNNARTR KGPRRTVANK KK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #19: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 7.263803 KDa
SequenceString:
MAKKSMIAKQ QRTPKFKVQE YTRCERCGRP HSVIRKFKLC RICFRELAYK GQIPGVKKAS W

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #20: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 10.597224 KDa
SequenceString:
MAITQERKNQ LINEFKTHES DTGSPEVQIA ILTDSINNLN EHLRTHKKDH HSRRGLLKMV GKRRNLLTYL RNKDVTRYRE LINKLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #21: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 10.153833 KDa
SequenceString:
MAVKIRLKRM GAKKSPFYRI VVADSRSPRD GRFIETVGTY NPVAKPAEVK IDEELALKWL QTGAKPSDTV RNLFSSQGIM EKFHNAKQG K

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #22: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 10.220979 KDa
SequenceString:
MSERNQRKVY QGRVVSDKMD KTITVVVETY KKHTLYGKRV KYSKKFKAHD ENNQAKIGDI VKIMETRPLS ATKRFRLVEV VEEAVII

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #23: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 8.990613 KDa
SequenceString:
MAGGRRGGRA KRRKVCYFTS NGITHIDYKD VDLLKKFVSE RGKILPRRVT GTNAKYQRKL TAAIKRARQM ALLPYVSGE

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #24: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 10.607309 KDa
SequenceString:
MARSLKKGPF VDGHLMTKIE KLNETDKKQV VKTWSRRSTI FPQFIGHTIA VYDGRKHVPV FISEDMVGHK LGEFAPTRTY KGHASDDKK TRR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #25: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 9.622217 KDa
SequenceString:
MPNIKSAIKR TKTNNERRVH NATIKSAMRT AIKQVEASVA NNEADKAKTA LTEAAKRIDK AVKTGLVHKN TAARYKSRLA KKVNGLSA

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #28: Endonuclease MutS2

MacromoleculeName: Endonuclease MutS2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 87.530852 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MQQKVLSALE FHKVKEQVIG HAASSLGKEM LLELKPSASI DEIKKQLDEV DEASDIIRLR GQAPFGGLVD IRGALRRAEI GSVLSPSEF TEISGLLYAV KQMKHFITQM AEDGVDIPLI HQHAEQLITL SDLERDINSC IDDHGEVLDH ASETLRGIRT Q LRTLESRV ...String:
MQQKVLSALE FHKVKEQVIG HAASSLGKEM LLELKPSASI DEIKKQLDEV DEASDIIRLR GQAPFGGLVD IRGALRRAEI GSVLSPSEF TEISGLLYAV KQMKHFITQM AEDGVDIPLI HQHAEQLITL SDLERDINSC IDDHGEVLDH ASETLRGIRT Q LRTLESRV RDRLESMLRS SSASKMLSDT IVTIRNDRFV IPVKQEYRSS YGGIVHDTSS SGATLFIEPQ AIVDMNNSLQ QA KVKEKQE IERILRVLTE KTAEYTEELF LDLQVLQTLD FIFAKARYAK AVKATKPIMN DTGFIRLKKA RHPLLPPDQV VAN DIELGR DFSTIVITGP NTGGKTVTLK TLGLLTLMAQ SGLHIPADEG SEAAVFEHVF ADIGDEQSIE QSLSTFSSHM VNIV GILEQ VNENSLVLFD ELGAGTDPQE GAALAMSILD DVHRTNARVL ATTHYPELKA YGYNREGVMN ASVEFDIETL SPTYK LLIG VPGRSNAFEI SKRLGLPDHI IGQAKSEMTA EHNEVDTMIA SLEQSKKRAE EELSETESIR KEAEKLHKEL QQQIIE LNS KKDKMLEEAE QQAAEKVKAA MKEAEDIIHE LRTIKEEHKS FKDHELINAK KRLEGAMPAF EKSKKPEKPK AQKRDFK PG DEVKVLTFGQ KGTLLEKTGG NEWNVQIGIL KMKVKEKDLE FIKSAPEQKK EKIITAVKGK DYHVSLELDL RGERYENA L SRVEKYLDDA VLAGYPRVSI IHGKGTGALR KGVQDLLKNH RSVKSSRFGE AGEGGSGVTV VELK

UniProtKB: Endonuclease MutS2

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Macromolecule #30: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 30.07475 KDa
SequenceString: AIKKYKPTSN GRRGMTTSDF AEITTDKPEK SLLAPLHKKG GRNNQGKLTV RHQGGGHKRQ YRVIDFKRDK DGIPGRVATV EYDPNRSAN IALINYADGE KRYILAPKGI QVGTEIMSGP EADIKVGNAL PLINIPVGTV VHNIELKPGK GGQLVRSAGT S AQVLGKEG ...String:
AIKKYKPTSN GRRGMTTSDF AEITTDKPEK SLLAPLHKKG GRNNQGKLTV RHQGGGHKRQ YRVIDFKRDK DGIPGRVATV EYDPNRSAN IALINYADGE KRYILAPKGI QVGTEIMSGP EADIKVGNAL PLINIPVGTV VHNIELKPGK GGQLVRSAGT S AQVLGKEG KYVLVRLNSG EVRMILSACR ASIGQVGNEQ HELINIGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRKSPMS PWGKPTLGFK TRKKKNKSDK FIVRRRKN

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #31: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 22.462973 KDa
SequenceString: TKGILGRKIG MTQVFAENGD LIPVTVIEAA PNVVLQKKTA ENDGYEAIQL GFDDKREKLS NKPEKGHVAK AETAPKRFVK ELRGVEMDA YEVGQEVKVE IFSAGEIVDV TGVSKGKGFQ GAIKRHGQSR GPMSHGSRYH RRPGSMGPVD PNRVFKGKLL P GRMGGEQI ...String:
TKGILGRKIG MTQVFAENGD LIPVTVIEAA PNVVLQKKTA ENDGYEAIQL GFDDKREKLS NKPEKGHVAK AETAPKRFVK ELRGVEMDA YEVGQEVKVE IFSAGEIVDV TGVSKGKGFQ GAIKRHGQSR GPMSHGSRYH RRPGSMGPVD PNRVFKGKLL P GRMGGEQI TVQNLEIVKV DAERNLLLIK GNVPGAKKSL ITVKSAVKS

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #32: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 22.222676 KDa
SequenceString: PKVALYNQNG STAGDIELNA SVFGIEPNES VVFDAILMQR ASLRQGTHKV KNRSEVRGGG RKPWRQKGTG RARQGSIRSP QWRGGGVVF GPTPRSYSYK LPKKVRRLAI KSVLSSKVID NNIIVLEDLT LDTAKTKEMA AILKGLSVEK KALIVTADAN E AVALSARN ...String:
PKVALYNQNG STAGDIELNA SVFGIEPNES VVFDAILMQR ASLRQGTHKV KNRSEVRGGG RKPWRQKGTG RARQGSIRSP QWRGGGVVF GPTPRSYSYK LPKKVRRLAI KSVLSSKVID NNIIVLEDLT LDTAKTKEMA AILKGLSVEK KALIVTADAN E AVALSARN IPGVTVVEAN GINVLDVVNH EKLLITKAAV EKVEEVL

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #33: Large ribosomal subunit protein uL5

MacromoleculeName: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 20.046371 KDa
SequenceString:
NRLKEKYNKE IAPALMTKFN YDSVMQVPKI EKIVINMGVG DAVQNAKAID SAVEELTFIA GQKPVVTRAK KSIAGFRLRE GMPIGAKVT LRGERMYDFL DKLISVSLPR VRDFRGVSKK SFDGRGNYTL GIKEQLIFPE IDYDKVTKVR GMDIVIVTTA N TDEEAREL LTQVGMPFQK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #34: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 19.124855 KDa
SequenceString:
SRVGKKLLEI PSDVTVTLND NNTVAVKGPK GELTRTFHPD MEIKVEDNVL TVARPSDQKE HRALHGTTRS LLGNMVEGVS KGFERGLEL VGVGYRASKS GNKLVLNVGY SHPVEIVPEE GIEIEVPSQT KVVVKGTDKE RVGAIAANIR AVRSPEPYKG K GIRYEGEV VRRKEGK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #35: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 16.01761 KDa
SequenceString:
TPMANASTIE RKWLVVDAAG KTLGRLSSEV AAILRGKHKP TYTPHVDTGD HVIIINAEKI ELTGKKLTDK IYYRHTQHPG GLKSRTALE MRTNYPEKML ELAIKGMLPK GSLGRQMFKK LNVYRGSEHP HEAQKPEVYE LRG

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #36: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 13.175288 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKQATPGG VVKKGEVVKA VIVRTKSGAR RSDGSYISFD ENACVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVI

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #37: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 15.410694 KDa
SequenceString:
MKLHELKPSE GSRKTRNRVG RGIGSGNGKT AGKGHKGQNA RSGGGVRPGF EGGQMPLFQR LPKRGFTNIN RKEYAVVNLD KLNGFAEGT EVTPELLLET GVISKLNAGV KILGNGKLEK KLTVKANKFS ASAKEAVEAA GGTAEVI

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #38: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 15.619512 KDa
SequenceString:
MLLPKRVKYR REHRGKMRGR AKGGTEVHFG EFGIQALEAS WITNRQIEAA RIAMTRYMKR GGKVWIKIFP SKPYTAKPLE VRMGSGKGA PEGWVAVVKP GKVLFEISGV SEEVAREALR LASHKLPIKT KFVKREEIG

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #39: Large ribosomal subunit protein bL17

MacromoleculeName: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 13.643609 KDa
SequenceString:
SYRKLGRTSA QRKAMLRDLT TDLIINERIE TTETRAKELR SVVEKMITLG KRGDLHARRQ AAAYIRNEVA NEENNQDALQ KLFSDIATR YEERQGGYTR IMKLGPRRGD GAPMAIIELV

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #40: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 12.993829 KDa
SequenceString:
MITKTSKNAA RLKRHARVRA KLSGTAERPR LNVFRSNKHI YAQIIDDVNG VTLASASTLD KDLNVESTGD TSAATKVGEL VAKRAAEKG ISDVVFDRGG YLYHGRVKAL ADAAREAGLK F

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #41: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 13.416853 KDa
SequenceString:
MQKLIEDITK EQLRTDLPAF RPGDTLRVHV KVVEGNRERI QIFEGVVIKR RGGGISETFT VRKISYGVGV ERTFPVHTPK IAKIEVVRY GKVRRAKLYY LRELRGKAAR IKEIRR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #42: Large ribosomal subunit protein bL20

MacromoleculeName: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 13.408813 KDa
SequenceString:
PRVKGGTVTR KRRKKVLKLA KGYFGSKHTL YKVANQQVMK SGNYAFRDRR QKKRDFRKLW ITRINAAARM NGLSYSRLMH GLKLSGIEV NRKMLADLAV NDLTAFNQLA DAAKAQLN

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #43: Large ribosomal subunit protein bL21

MacromoleculeName: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 11.164886 KDa
SequenceString:
YAIIKTGGKQ IKVEEGQTVY IEKLAAEAGE TVTFEDVLFV GGDNVKVGNP TVEGATVTAK VEKQGRAKKI TVFRYKPKKN VHKKQGHRQ PYTKVTIEKI NA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #44: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 12.035066 KDa
SequenceString:
QAKAVARTVR IAPRKARLVM DLIRGKQVGE AVSILNLTPR AASPIIEKVL KSAIANAEHN YEMDANNLVI SQAFVDEGPT LKRFRPRAM GRASQINKRT SHITIVVSEK

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #45: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 10.778622 KDa
SequenceString:
MKDPRDVLKR PVITERSADL MTEKKYTFEV DVRANKTEVK DAVESIFGVK VDKVNIMNYK GKSKRVGRYT GMTSRRRKAI VKLTADSKE IEIF

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #46: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 10.921854 KDa
SequenceString:
MHVKKGDKVM VISGKDKGKQ GTILAAFPKK DRVLVEGVNM VKKHSKPTQA NPQGGISNQE APIHVSNVMP LDPKTGEVTR VGYKVEDGK KVRVAKKSGQ VL

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #47: Large ribosomal subunit protein bL27

MacromoleculeName: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 8.956143 KDa
SequenceString:
SKKGVGSTKN GRDSEAKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGTVKFERF GRDRKKVSVY PV

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #48: Large ribosomal subunit protein bL28

MacromoleculeName: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 6.367546 KDa
SequenceString:
RKCVITGKKT TAGNNRSHAM NASKRTWGAN LQKVRILVNG KPKKVYVSAR ALKSGKVE

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #49: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 7.598849 KDa
SequenceString:
MKANEIRDLT TAEIEQKVKS LKEELFNLRF QLATGQLENT ARIREVRKAI ARMKTVIRER EIAAN

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #50: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 6.519599 KDa
SequenceString:
AKLEITLKRS VIGRPEDQRV TVRTLGLKKT NQTVVHEDNA AIRGMINKVS HLVSVKEQ

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #51: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 16.382967 KDa
SequenceString:
MKVIFLQDVK GKGKKGEVKN VADGYAHNFL IKKGLAVEAN ASNISALNGQ KQKEKKEAIA ELEQAKSLKE TLEKLTVELS AKSGEGGRL FGSVTSKQIT EQLQKDHNIK VDKRKLELPD GIRALGYTNV PVKLHPEVQA VLKVHVKEEA

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #26: tRNA (77-MER)

MacromoleculeName: tRNA (77-MER) / type: rna / ID: 26 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 24.815682 KDa
SequenceString:
GGUCCGGUAG UUCAGUUGGU UAGAAUGCCU GCCUGUCACG CAGGAGGUCG CGGGUUCGAG UCCCGUCCGG ACCGCCA

GENBANK: GENBANK: CP031784.1

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Macromolecule #27: mRNA (33-MER)

MacromoleculeName: mRNA (33-MER) / type: rna / ID: 27 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 10.591393 KDa
SequenceString:
AGACCAAUGC CAUGUACGUC GCACUCGGAA GAC

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Macromolecule #29: 5S rRNA (112-MER)

MacromoleculeName: 5S rRNA (112-MER) / type: rna / ID: 29 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 36.15752 KDa
SequenceString:
UGGUGGCGAU AGCGAAGAGG UCACACCCGU UCCCAUACCG AACACGGAAG UUAAGCUCUU CAGCGCCGAU GGUAGUCGGG GGUUUCCCC CUGUGAGAGU AGGACGCCGC CAA

GENBANK: GENBANK: CP035165.1

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Macromolecule #52: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 52 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 949.646312 KDa
SequenceString: GGUUAAGUUA GAAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACGAACACCG AUAUGCUUCG GGGAGCUGU AAGCAAGCUU UGAUCCGGAG AUUUCCGAAU GGGGAAACCC ACCACUCGUA AUGGAGUGGU AUCCAUAUCU G AAUUCAUA ...String:
GGUUAAGUUA GAAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACGAACACCG AUAUGCUUCG GGGAGCUGU AAGCAAGCUU UGAUCCGGAG AUUUCCGAAU GGGGAAACCC ACCACUCGUA AUGGAGUGGU AUCCAUAUCU G AAUUCAUA GGAUAUGAGA AGGCAGACCC GGGGAACUGA AACAUCUAAG UACCCGGAGG AAGAGAAAGC AAAUGCGAUU CC CUGAGUA GCGGCGAGCG AAACGGGAUC AGCCCAAACC AAGAGGCUUG CCUCUUGGGG UUGUAGGACA CUCUGUACGG AGU UACAAA GGAACGAGGU AGAUGAAGAG GUCUGGAAAG GCCCGCCAUA GGAGGUAACA GCCCUGUAGU CAAAACUUCG UUCU CUCCU GAGUGGAUCC UGAGUACGGC GGAACACGUG AAAUUCCGUC GGAAUCCGGG AGGACCAUCU CCCAAGGCUA AAUAC UCCC UAGUGACCGA UAGUGAACCA GUACCGUGAG GGAAAGGUGA AAAGCACCCC GGAAGGGGAG UGAAAGAGAU CCUGAA ACC GUGUGCCUAC AAGUAGUCAG AGCCCGUUAA CGGGUGAUGG CGUGCCUUUU GUAGAAUGAA CCGGCGAGUU ACGAUCU CG UGCAAGGUUA AGCAGAAGAU GCGGAGCCGC AGCGAAAGCG AGUCUGAAUA GGGCGCAUGA GUACGUGGUC GUAGACCC G AAACCAGGUG AUCUACCCAU GUCCAGGGUG AAGUUCAGGU AACACUGAAU GGAGGCCCGA ACCCACGCAC GUUGAAAAG UGCGGGGAUG AGGUGUGGGU AGGGGUGAAA UGCCAAUCGA ACCUGGAGAU AGCUGGUUCU CUCCGAAAUA GCUUUAGGGC UAGCCUCAA GGUAAGAGUC UUGGAGGUAG AGCACUGAUU GGACUAGGGG CCCCUACCGG GUUACCGAAU UCAGUCAAAC U CCGAAUGC CAAUGACUUA UCCUUGGGAG UCAGACUGCG AGUGAUAAGA UCCGUAGUCG AAAGGGAAAC AGCCCAGACC GC CAGCUAA GGUCCCAAAG UAUACGUUAA GUGGAAAAGG AUGUGGAGUU GCUUAGACAA CCAGGAUGUU GGCUUAGAAG CAG CCACCA UUUAAAGAGU GCGUAAUAGC UCACUGGUCG AGUGACUCUG CGCCGAAAAU GUACCGGGGC UAAACGUAUC ACCG AAGCU GCGGACUGUU CUUCGAACAG UGGUAGGAGA GCGUUCUAAG GGCUGUGAAG CCAGACCGGA AGGACUGGUG GAGCG CUUA GAAGUGAGAA UGCCGGUAUG AGUAGCGAAA GAGGGGUGAG AAUCCCCUCC ACCGAAUGCC UAAGGUUUCC UGAGGA AGG CUCGUCCGCU CAGGGUUAGU CGGGACCUAA GCCGAGGCCG AAAGGCGUAG GCGAUGGACA ACAGGUUGAU AUUCCUG UA CCACCUCCUC ACCAUUUGAG CAAUGGGGGG ACGCAGGAGG AUAGGGUAAG CGCGGUAUUG GAUAUCCGCG UCCAAGCA G UUAGGCUGGG AAAUAGGCAA AUCCGUUUCC CAUAAGGCUG AGCUGUGAUG GCGAGCGAAA UAUAGUAGCG AAGUUCCUG AUUCCACACU GCCAAGAAAA GCCUCUAGCG AGGUGAGAGG UGCCCGUACC GCAAACCGAC ACAGGUAGGC GAGGAGAGAA UCCUAAGGU GAUCGAGAGA ACUCUCGUUA AGGAACUCGG CAAAAUGACC CCGUAACUUC GGGAGAAGGG GUGCUCUGUU A GGGUGCAA GCCCGAGAGA GCCGCAGUGA AUAGGCCCAG GCGACUGUUU AGCAAAAACA CAGGUCUCUG CGAAGCCGUA AG GCGAAGU AUAGGGGCUG ACGCCUGCCC GGUGCUGGAA GGUUAAGAGG AGCGCUUAGC GUAAGCGAAG GUGCGAAUUG AAG CCCCAG UAAACGGCGG CCGUAACUAU AACGGUCCUA AGGUAGCGAA AUUCCUUGUC GGGUAAGUUC CGACCCGCAC GAAA GGCGC AACGAUCUGG GCACUGUCUC AACGAGAGAC UCGGUGAAAU UAUAGUACCU GUGAAGAUGC AGGUUACCCG CGACA GGAC GGAAAGACCC CGUGGAGCUU UACUGCAGCC UGAUAUUGAA UGUUGGUACA GCUUGUACAG GAUAGGUAGG AGCCUU GGA AACCGGAGCG CCAGCUUCGG UGGAGGCAUC GGUGGGAUAC UACCCUGGCU GUAUUGACCU UCUAACCCGC CGCCCUU AU CGGGCGGGGA GACAGUGUCA GGUGGGCAGU UUGACUGGGG CGGUCGCCUC CUAAAAGGUA ACGGAGGCGC CCAAAGGU U CCCUCAGAAU GGUUGGAAAU CAUUCGCAGA GUGUAAAGGC ACAAGGGAGC UUGACUGCGA GACCUACAAG UCGAGCAGG GACGAAAGUC GGGCUUAGUG AUCCGGUGGU UCCGCAUGGA AGGGCCAUCG CUCAACGGAU AAAAGCUACC CCGGGGAUAA CAGGCUUAU CUCCCCCAAG AGUCCACAUC GACGGGGAGG UUUGGCACCU CGAUGUCGGC UCAUCGCAUC CUGGGGCUGU A GUCGGUCC CAAGGGUUGG GCUGUUCGCC CAUUAAAGCG GUACGCGAGC UGGGUUCAGA ACGUCGUGAG ACAGUUCGGU CC CUAUCCG UCGCGGGCGC AGGAAAUUUG AGAGGAGCUG UCCUUAGUAC GAGAGGACCG GGAUGGACGC ACCGCUGGUG UAC CAGUUG UUCUGCCAAG GGCAUCGCUG GGUAGCUAUG UGCGGACGGG AUAAGUGCUG AAAGCAUCUA AGCAUGAAGC CCCC CUCAA GAUGAGAUUU CCCAUUCCGC AAGGAAGUAA GAUCCCUGAA AGAUGAUCAG GUUGAUAGGU CUGAGGUGGA AGUGU GGCG ACACAUGGAG CUGACAGAUA CUAAUCGAUC GAGGACUUAA CCAU

GENBANK: GENBANK: AP024621.1

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Macromolecule #53: 16S rRNA (1533-MER)

MacromoleculeName: 16S rRNA (1533-MER) / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis (bacteria)
Molecular weightTheoretical: 496.854188 KDa
SequenceString: GGAGAGUUUG AUCCUGGCUC AGGACGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGGACAGAU GGGAGCUUGC UCCCUGAUG UUAGCGGCGG ACGGGUGAGU AACACGUGGG UAACCUGCCU GUAAGACUGG GAUAACUCCG GGAAACCGGG G CUAAUACC ...String:
GGAGAGUUUG AUCCUGGCUC AGGACGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGGACAGAU GGGAGCUUGC UCCCUGAUG UUAGCGGCGG ACGGGUGAGU AACACGUGGG UAACCUGCCU GUAAGACUGG GAUAACUCCG GGAAACCGGG G CUAAUACC GGAUGGUUGU UUGAACCGCA UGGUUCAAAC AUAAAAGGUG GCUUCGGCUA CCACUUACAG AUGGACCCGC GG CGCAUUA GCUAGUUGGU GAGGUAACGG CUCACCAAGG CGACGAUGCG UAGCCGACCU GAGAGGGUGA UCGGCCACAC UGG GACUGA GACACGGCCC AGACUCCUAC GGGAGGCAGC AGUAGGGAAU CUUCCGCAAU GGACGAAAGU CUGACGGAGC AACG CCGCG UGAGUGAUGA AGGUUUUCGG AUCGUAAAGC UCUGUUGUUA GGGAAGAACA AGUGCCGUUC GAAUAGGGCG GUACC UUGA CGGUACCUAA CCAGAAAGCC ACGGCUAACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGU GGCAAGCGUU GUCCGG AAU UAUUGGGCGU AAAGGGCUCG CAGGCGGUUU CUUAAGUCUG AUGUGAAAGC CCCCGGCUCA ACCGGGGAGG GUCAUUG GA AACUGGGGAA CUUGAGUGCA GAAGAGGAGA GUGGAAUUCC ACGUGUAGCG GUGAAAUGCG UAGAGAUGUG GAGGAACA C CAGUGGCGAA GGCGACUCUC UGGUCUGUAA CUGACGCUGA GGAGCGAAAG CGUGGGGAGC GAACAGGAUU AGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUAGGG GGUUUCCGCC CCUUAGUGCU GCAGCUAACG CAUUAAGCAC UCCGCCUGG GGAGUACGGU CGCAAGACUG AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA GCAUGUGGUU U AAUUCGAA GCAACGCGAA GAACCUUACC AGGUCUUGAC AUCCUCUGAC AAUCCUAGAG AUAGGACGUC CCCUUCGGGG GC AGAGUGA CAGGUGGUGC AUGGUUGUCG UCAGCUCGUG UCGUGAGAUG UUGGGUUAAG UCCCGCAACG AGCGCAACCC UUG AUCUUA GUUGCCAGCA UUCAGUUGGG CACUCUAAGG UGACUGCCGG UGACAAACCG GAGGAAGGUG GGGAUGACGU CAAA UCAUC AUGCCCCUUA UGACCUGGGC UACACACGUG CUACAAUGGA CAGAACAAAG GGCAGCGAAA CCGCGAGGUU AAGCC AAUC CCACAAAUCU GUUCUCAGUU CGGAUCGCAG UCUGCAACUC GACUGCGUGA AGCUGGAAUC GCUAGUAAUC GCGGAU CAG CAUGCCGCGG UGAAUACGUU CCCGGGCCUU GUACACACCG CCCGUCACAC CACGAGAGUU UGUAACACCC GAAGUCG GU GAGGUAACCU UUUAGGAGCC AGCCGCCGAA GGUGGGACAG AUGAUUGGGG UGAAGUCGUA ACAAGGUAGC CGUAUCGG A AGGUGCGGCU GG

GENBANK: GENBANK: CP058242.1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.4 µm
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 43.6 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 11740
FSC plot (resolution estimation)

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