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TitleStructure of Photosystem II Reveals Protective Mechanisms against Environmental Stress.
Journal, issue, pagesCells, Vol. 12, Issue 15, Year 2023
Publish dateJul 31, 2023
AuthorsMaria Fadeeva / Daniel Klaiman / Ido Caspy / Nathan Nelson /
PubMed AbstractGreen alga is known for its ability to carry out photosynthesis under harsh conditions. Using cryogenic electron microscopy (cryoEM), we obtained a high-resolution structure of PSII at 2.72 Å. This ...Green alga is known for its ability to carry out photosynthesis under harsh conditions. Using cryogenic electron microscopy (cryoEM), we obtained a high-resolution structure of PSII at 2.72 Å. This structure revealed 64 subunits, which encompassed 386 chlorophylls, 86 carotenoids, four plastoquinones, and several structural lipids. At the luminal side of PSII, a unique subunit arrangement was observed to protect the oxygen-evolving complex. This arrangement involved PsbO (OEE1), PsbP (OEE2), PsbB, and PsbU (a homolog of plant OEE3). PsbU interacted with PsbO, PsbC, and PsbP, thereby stabilizing the shield of the oxygen-evolving complex. Significant changes were also observed at the stromal electron acceptor side. PsbY, identified as a transmembrane helix, was situated alongside PsbF and PsbE, which enclosed cytochrome . Supported by the adjacent C-terminal helix of Psb10, these four transmembrane helices formed a bundle that shielded cytochrome from the surrounding solvent. Moreover, the bulk of Psb10 formed a protective cap, which safeguarded the quinone site and likely contributed to the stacking of PSII complexes. Based on our findings, we propose a protective mechanism that prevents Q (plastoquinone B) from becoming fully reduced. This mechanism offers insights into the regulation of electron transfer within PSII.
External linksCells / PubMed:37566050 / PubMed Central
MethodsEM (single particle)
Resolution2.73 Å
Structure data

15973

8bd3

EMDB-15973, PDB-8bd3:
Cryo-EM structure of the Photosystem II - LHCII supercomplex from Chlorella ohadi
Method: EM (single particle) / Resolution: 2.73 Å

Chemicals

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-RRX:
(3R)-beta,beta-caroten-3-ol / Β-Cryptoxanthin

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-OEX:
CA-MN4-O5 CLUSTER

ChemComp-FE2:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

ChemComp-PHO:
PHEOPHYTIN A / Pheophytin

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / Plastoquinone

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM / Bicarbonate

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

ChemComp-HOH:
WATER / Water

Source
  • chlorella ohadii (plant)
KeywordsPHOTOSYNTHESIS / Green alga / PSII / C.ohadi / membrane protein / Cryo-EM

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