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- EMDB-15973: Cryo-EM structure of the Photosystem II - LHCII supercomplex from... -

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Basic information

Entry
Database: EMDB / ID: EMD-15973
TitleCryo-EM structure of the Photosystem II - LHCII supercomplex from Chlorella ohadi
Map data
Sample
  • Complex: Chlorella ohadii Photosystem II
    • Protein or peptide: x 29 types
  • Ligand: x 20 types
KeywordsGreen alga / PSII / C.ohadi / membrane protein / Cryo-EM / PHOTOSYNTHESIS
Function / homology
Function and homology information


diacylglycerol kinase (ATP) / ATP-dependent diacylglycerol kinase activity / photosynthesis, light harvesting / photosystem II assembly / photosystem II stabilization / oxygen evolving activity / photosystem II oxygen evolving complex / photosystem II / photosystem II reaction center / protein kinase C-activating G protein-coupled receptor signaling pathway ...diacylglycerol kinase (ATP) / ATP-dependent diacylglycerol kinase activity / photosynthesis, light harvesting / photosystem II assembly / photosystem II stabilization / oxygen evolving activity / photosystem II oxygen evolving complex / photosystem II / photosystem II reaction center / protein kinase C-activating G protein-coupled receptor signaling pathway / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / photosystem I / response to herbicide / photosystem II / plastid / photosynthetic electron transport in photosystem II / extrinsic component of membrane / chlorophyll binding / chloroplast thylakoid membrane / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / photosynthesis / chloroplast / electron transfer activity / protein stabilization / iron ion binding / calcium ion binding / heme binding / RNA binding / ATP binding / membrane / metal ion binding
Similarity search - Function
Photosystem II PsbR / Photosystem II PsbY, plant / Photosystem II 10 kDa polypeptide PsbR / Diacylglycerol kinase, accessory domain / Diacylglycerol kinase accessory domain / Diacylglycerol kinase accessory domain (presumed) / Serine incorporator/TMS membrane protein / Serine incorporator (Serinc) / Diacylglycerol kinase, catalytic domain / Diacylglycerol kinase catalytic domain ...Photosystem II PsbR / Photosystem II PsbY, plant / Photosystem II 10 kDa polypeptide PsbR / Diacylglycerol kinase, accessory domain / Diacylglycerol kinase accessory domain / Diacylglycerol kinase accessory domain (presumed) / Serine incorporator/TMS membrane protein / Serine incorporator (Serinc) / Diacylglycerol kinase, catalytic domain / Diacylglycerol kinase catalytic domain / DAG-kinase catalytic (DAGKc) domain profile. / Diacylglycerol kinase catalytic domain (presumed) / Oxygen-evolving enhancer protein 3 / Oxygen evolving enhancer protein 3 (PsbQ) / PsbQ-like domain superfamily / Inorganic polyphosphate/ATP-NAD kinase, N-terminal / NAD kinase/diacylglycerol kinase-like domain superfamily / Photosystem II PsbJ / Photosystem II PsbJ superfamily / PsbJ / Photosystem II reaction centre protein Ycf12 / Photosystem II complex subunit Ycf12 / Photosystem II PsbO, manganese-stabilising / Manganese-stabilising protein / photosystem II polypeptide / Photosystem II reaction centre M protein (PsbM) / Photosystem II PsbM superfamily / Photosystem II PsbZ, reaction centre / Photosystem II PsbZ superfamily / YCF9 / Photosystem II PsbM / Photosystem II PsbT / Photosystem II PsbL / Photosystem II PsbL superfamily / Photosystem II PsbT superfamily / Photosystem II CP43 reaction centre protein superfamily / Photosystem II reaction centre T protein / PsbL protein / Photosystem II PsbK / Photosystem II CP43 reaction centre protein / Photosystem II PsbK superfamily / Photosystem II 4 kDa reaction centre component / Photosystem II CP47 reaction centre protein / Photosystem II PsbI / Photosystem II PsbI superfamily / Photosystem II reaction centre I protein (PSII 4.8 kDa protein) / Photosystem II reaction centre protein H / Photosystem II protein D1 / Photosystem II D2 protein / Photosystem II cytochrome b559, conserved site / Photosystem II cytochrome b559, alpha subunit / Photosystem II cytochrome b559, beta subunit / Photosystem II cytochrome b559, N-terminal / Photosystem II cytochrome b559, alpha subunit, lumenal region / Photosystem II reaction centre protein H superfamily / Photosystem II cytochrome b559, alpha subunit superfamily / Cytochrome b559, alpha (gene psbE) and beta (gene psbF)subunits / Lumenal portion of Cytochrome b559, alpha (gene psbE) subunit / Photosystem II 10 kDa phosphoprotein / Cytochrome b559 subunits heme-binding site signature. / KH domain / Photosystem antenna protein-like / Photosystem antenna protein-like superfamily / Photosystem II protein / Chlorophyll A-B binding protein, plant and chromista / Chlorophyll A-B binding protein / Chlorophyll A-B binding protein / K Homology domain, type 1 / Outer membrane protein/outer membrane enzyme PagP, beta-barrel / Type-1 KH domain profile. / K Homology domain, type 1 superfamily / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature.
Similarity search - Domain/homology
Photosystem II CP43 reaction center protein / Photosystem II reaction center protein Ycf12 / Chlorophyll a-b binding protein, chloroplastic / Serine incorporator / Multifunctional fusion protein / Chloroplast photosystem II 10 kDa protein / Photosystem II reaction center protein M / Chloroplast oxygen-evolving enhancer protein 3 / Photosystem II reaction center protein I / Chloroplast PsbY ...Photosystem II CP43 reaction center protein / Photosystem II reaction center protein Ycf12 / Chlorophyll a-b binding protein, chloroplastic / Serine incorporator / Multifunctional fusion protein / Chloroplast photosystem II 10 kDa protein / Photosystem II reaction center protein M / Chloroplast oxygen-evolving enhancer protein 3 / Photosystem II reaction center protein I / Chloroplast PsbY / Chloroplast oxygen-evolving enhancer protein 1 / Cytochrome b559 subunit beta / Photosystem II reaction center protein T / Photosystem II reaction center protein L / Photosystem II reaction center protein K / Photosystem II protein D1 / Photosystem II reaction center protein H / Photosystem II reaction center protein Z / Cytochrome b559 subunit alpha / Photosystem II D2 protein / Photosystem II reaction center protein J / Photosystem II CP47 reaction center protein
Similarity search - Component
Biological speciesChlorella ohadii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.73 Å
AuthorsFadeeva M / Klaiman D / Caspy I / Nelson N
Funding support Israel, 1 items
OrganizationGrant numberCountry
Israel Science Foundation199/21 Israel
CitationJournal: Cells / Year: 2023
Title: Structure of Photosystem II Reveals Protective Mechanisms against Environmental Stress.
Authors: Maria Fadeeva / Daniel Klaiman / Ido Caspy / Nathan Nelson /
Abstract: Green alga is known for its ability to carry out photosynthesis under harsh conditions. Using cryogenic electron microscopy (cryoEM), we obtained a high-resolution structure of PSII at 2.72 Å. This ...Green alga is known for its ability to carry out photosynthesis under harsh conditions. Using cryogenic electron microscopy (cryoEM), we obtained a high-resolution structure of PSII at 2.72 Å. This structure revealed 64 subunits, which encompassed 386 chlorophylls, 86 carotenoids, four plastoquinones, and several structural lipids. At the luminal side of PSII, a unique subunit arrangement was observed to protect the oxygen-evolving complex. This arrangement involved PsbO (OEE1), PsbP (OEE2), PsbB, and PsbU (a homolog of plant OEE3). PsbU interacted with PsbO, PsbC, and PsbP, thereby stabilizing the shield of the oxygen-evolving complex. Significant changes were also observed at the stromal electron acceptor side. PsbY, identified as a transmembrane helix, was situated alongside PsbF and PsbE, which enclosed cytochrome . Supported by the adjacent C-terminal helix of Psb10, these four transmembrane helices formed a bundle that shielded cytochrome from the surrounding solvent. Moreover, the bulk of Psb10 formed a protective cap, which safeguarded the quinone site and likely contributed to the stacking of PSII complexes. Based on our findings, we propose a protective mechanism that prevents Q (plastoquinone B) from becoming fully reduced. This mechanism offers insights into the regulation of electron transfer within PSII.
History
DepositionOct 18, 2022-
Header (metadata) releaseAug 9, 2023-
Map releaseAug 9, 2023-
UpdateAug 23, 2023-
Current statusAug 23, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15973.png

Downloads & links

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Map

FileDownload / File: emd_15973.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.92 Å/pix.
x 500 pix.
= 460.8 Å		0.92 Å/pix.
x 500 pix.
= 460.8 Å		0.92 Å/pix.
x 500 pix.
= 460.8 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.9216 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.008
Minimum - Maximum-0.027582256 - 0.066376135
Average (Standard dev.)0.00021178843 (±0.0023458973)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 460.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_15973_half_map_1.map
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Half map: #1

Fileemd_15973_half_map_2.map
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Sample components

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Entire : Chlorella ohadii Photosystem II

EntireName: Chlorella ohadii Photosystem II
Components
  • Complex: Chlorella ohadii Photosystem II
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding of LHCII
    • Protein or peptide: Chlorophyll a-b binding of LHCII
    • Protein or peptide: Photosystem II protein D1
    • Protein or peptide: Photosystem II CP47 reaction center protein
    • Protein or peptide: Photosystem II CP43 reaction center protein
    • Protein or peptide: Photosystem II D2 protein
    • Protein or peptide: Cytochrome b559 subunit alpha
    • Protein or peptide: Cytochrome b559 subunit beta
    • Protein or peptide: Photosystem II reaction center protein H
    • Protein or peptide: Photosystem II reaction center protein I
    • Protein or peptide: Photosystem II reaction center protein J
    • Protein or peptide: Photosystem II reaction center protein K
    • Protein or peptide: Photosystem II reaction center protein L
    • Protein or peptide: Photosystem II reaction center protein M
    • Protein or peptide: Chloroplast oxygen-evolving enhancer protein 1
    • Protein or peptide: Chlorophyll a b binding CP29
    • Protein or peptide: Chlorophyll a b-binding CP26
    • Protein or peptide: Photosystem II reaction center protein T
    • Protein or peptide: Photosystem II reaction center protein Ycf12
    • Protein or peptide: Photosystem II reaction center W protein
    • Protein or peptide: Photosystem II reaction center protein X
    • Protein or peptide: Multifunctional fusion protein
    • Protein or peptide: Photosystem II reaction center protein Z
    • Protein or peptide: Chloroplast oxygen-evolving enhancer protein 3
    • Protein or peptide: Chloroplast PsbY
    • Protein or peptide: Chloroplast photosystem II 10 kDa protein
    • Protein or peptide: Photosystem II oxygen evolving enhancer 2
  • Ligand: CHLOROPHYLL B
  • Ligand: CHLOROPHYLL A
  • Ligand: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: (3R)-beta,beta-caroten-3-ol
  • Ligand: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
  • Ligand: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
  • Ligand: CA-MN4-O5 CLUSTER
  • Ligand: FE (II) ION
  • Ligand: CHLORIDE IONChloride
  • Ligand: PHEOPHYTIN APheophytin
  • Ligand: BETA-CAROTENEΒ-Carotene
  • Ligand: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: BICARBONATE IONBicarbonate
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: DODECYL-ALPHA-D-MALTOSIDE
  • Ligand: water

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Supramolecule #1: Chlorella ohadii Photosystem II

SupramoleculeName: Chlorella ohadii Photosystem II / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#29
Source (natural)Organism: Chlorella ohadii (plant) / Organelle: Chloroplast / Location in cell: Thylakoid membrane
Molecular weightTheoretical: 1.7 MDa

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Macromolecule #1: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 23.060059 KDa
SequenceString: SQWYGPDRPK FLGPYSTNFV PSYLTGEFPG DYGWDTSGLS ADPATFARYR EIEVIHARWA MLGALGCVVP ELLDGTNHIA WFKAGAEIF GDDGIQYLGI PGLINAKSIV ATLIVQVVLM SYVEGYRVNG GPAGTGLDAV YPGGNFDPLG LADDPDTLAE L KVKEIKNG ...String:
SQWYGPDRPK FLGPYSTNFV PSYLTGEFPG DYGWDTSGLS ADPATFARYR EIEVIHARWA MLGALGCVVP ELLDGTNHIA WFKAGAEIF GDDGIQYLGI PGLINAKSIV ATLIVQVVLM SYVEGYRVNG GPAGTGLDAV YPGGNFDPLG LADDPDTLAE L KVKEIKNG RLAMLSMFGF FIQAIVTGEG PIKNLNDHLA DASANNFYAL YAKNLA

UniProtKB: Serine incorporator

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Macromolecule #2: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 23.850742 KDa
SequenceString: EFYGPDRAKW LGPFSEGSVP SYLKGEFPGD YGWDTAGLSA DPETFARYRE IEVIHARWAM LGALGCLTPE LLQNNGAADF GEAAVWFKA GASIFNEDGL NYLGNPSLIH AQSIVAVLLT QLVIMGAAEA YRYSGEGPVE TSGDSLYPGG FFDPLGLADD P DTLAELKV ...String:
EFYGPDRAKW LGPFSEGSVP SYLKGEFPGD YGWDTAGLSA DPETFARYRE IEVIHARWAM LGALGCLTPE LLQNNGAADF GEAAVWFKA GASIFNEDGL NYLGNPSLIH AQSIVAVLLT QLVIMGAAEA YRYSGEGPVE TSGDSLYPGG FFDPLGLADD P DTLAELKV KEIKNGRLAM FSMFGFFVQG LVTGKSPLEN LADHLAEPSV NNGFASATKF LPLP

UniProtKB: Chlorophyll a-b binding protein, chloroplastic

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Macromolecule #3: Chlorophyll a-b binding of LHCII

MacromoleculeName: Chlorophyll a-b binding of LHCII / type: protein_or_peptide / ID: 3 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 23.847945 KDa
SequenceString: VEWYGPNRPQ FLGPFSNPPS YLKGEFPGDY GWDTAGLSAD PETFARYREL EVIHARWAML GALGCVTPEL LQRNGVANFG EAVWFKAGA QIFQEGGLDY LGNPSLIHAQ SIVAILLTQL VLMGLIEGYR VNGGPAGEGL DALYPGEAFD PLGLADDPDT F AELKVKEL ...String:
VEWYGPNRPQ FLGPFSNPPS YLKGEFPGDY GWDTAGLSAD PETFARYREL EVIHARWAML GALGCVTPEL LQRNGVANFG EAVWFKAGA QIFQEGGLDY LGNPSLIHAQ SIVAILLTQL VLMGLIEGYR VNGGPAGEGL DALYPGEAFD PLGLADDPDT F AELKVKEL KNGRLAMVSM LGFGVQGLIT RKGPIENLAD HLAAPDATNF WVDYAPKSAG IGY

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Macromolecule #4: Chlorophyll a-b binding of LHCII

MacromoleculeName: Chlorophyll a-b binding of LHCII / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 23.45549 KDa
SequenceString: EFYGPDRVKF LGPFSGNTPS YLKGEYPGDY GWDTAGLSAD PETFARYREL EVIHARWAML GALGCVTPEL LQRNGAANFG EAVWFKAGA QIFQEGGLDY LGNPNLVHAQ SIVAILLTQV VLMGLIEGYR VNGGPAGEVV DPIYPGEAFD PLGLADDPDT L AELKVKEI ...String:
EFYGPDRVKF LGPFSGNTPS YLKGEYPGDY GWDTAGLSAD PETFARYREL EVIHARWAML GALGCVTPEL LQRNGAANFG EAVWFKAGA QIFQEGGLDY LGNPNLVHAQ SIVAILLTQV VLMGLIEGYR VNGGPAGEVV DPIYPGEAFD PLGLADDPDT L AELKVKEI KNGRLAMVSM FGFFIQAIVT GKGPVQNLSD HLASPSTDNF YSLYAKTLA

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Macromolecule #5: Photosystem II protein D1

MacromoleculeName: Photosystem II protein D1 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant) / Strain: 1
Molecular weightTheoretical: 37.23032 KDa
SequenceString: ESTSLWARFC EWVTSTENRL YIGWFGVLMI PTLLTATSVF IIAFIAAPPV DIDGIREPVS GSLLYGNNII TGAIIPTSNA IGLHFYPIW EAASLDEWLY NGGPYQLIVC HFFLGVCSYM GREWELSFRL GMRPWIAVAY SAPVAAATAV FIIYPLGQGS F SDGMPLGI ...String:
ESTSLWARFC EWVTSTENRL YIGWFGVLMI PTLLTATSVF IIAFIAAPPV DIDGIREPVS GSLLYGNNII TGAIIPTSNA IGLHFYPIW EAASLDEWLY NGGPYQLIVC HFFLGVCSYM GREWELSFRL GMRPWIAVAY SAPVAAATAV FIIYPLGQGS F SDGMPLGI SGTFNFMIVF QAEHNILMHP FHMLGVAGVF GGSLFSAMHG SLVTSSLIRE TTENESANEG YKFGQEEETY NI VAAHGYF GRLIFQYASF NNSRSLHFFL AAWPVVGIWF TALGISTMAF NLNGFNFNQS VVDSQGRVIN TWADIINRAN LGM EVMHER NAHNFPLDLA

UniProtKB: Photosystem II protein D1

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Macromolecule #6: Photosystem II CP47 reaction center protein

MacromoleculeName: Photosystem II CP47 reaction center protein / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 55.75427 KDa
SequenceString: GLPWYRVHTV VLNDPGRLIA VHLMHTSLVS GWAGSMAFYE LAVFDPSDPV LNPMWRQGMF VLPFMTRLGI TQSWGGWTIS GETASNPGI WSYEGVAAAH IILSGLLFAA SIWHWVYWDL ELFRDPRTSN PALDLPKIFG IHLFLSGLLC FGFGAFHVTG L FGPGIWVS ...String:
GLPWYRVHTV VLNDPGRLIA VHLMHTSLVS GWAGSMAFYE LAVFDPSDPV LNPMWRQGMF VLPFMTRLGI TQSWGGWTIS GETASNPGI WSYEGVAAAH IILSGLLFAA SIWHWVYWDL ELFRDPRTSN PALDLPKIFG IHLFLSGLLC FGFGAFHVTG L FGPGIWVS DPYGITGTVQ AVAPSWDATG FDPYNPGGIS AHHIAAGILG VLAGLFHLCV RPPQRLYNGL RMGNIETVLS SS IAAVFWA AFVVSGTMWY GSAATPIELF GPTRYQWDLG FFQQEIERRV QTSLAEGKSA SQAWAEIPEK LAFYDYIGNN PAK GGLFRA GAMNSGDGIA VGWLGHAVFK DKQGNELFVR RMPTFFETFP VVLLDKDGVV RADVPFRRSE SKYSIEQVGV SVTF YGGEL DGVTFSDPAT VKKYARRAQL GEIFEFDRAT LQSDGVFRTS PRGWFTFAHL CFALLFFFGH IWHGARTIFR DVFAG IDAD LDEQVEFGAF LKLGDTSTRR

UniProtKB: Photosystem II CP47 reaction center protein

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Macromolecule #7: Photosystem II CP43 reaction center protein

MacromoleculeName: Photosystem II CP43 reaction center protein / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 49.161105 KDa
SequenceString: GRDQESTGFA WWAGNARLIN LSGKLLGAHV AHAGLIVFWA GAMNLFEVAH FVPEKPMYEQ GLILLPHLAT LGYGVGPGGE VIDTYPYFV SGVLHLISSA VLGFGGVYHS LVGPETLEES FPFFGYVWKD KNKMTTILGI HLIVLGLGAW LLVWKAMYFG G IYDTWAPG ...String:
GRDQESTGFA WWAGNARLIN LSGKLLGAHV AHAGLIVFWA GAMNLFEVAH FVPEKPMYEQ GLILLPHLAT LGYGVGPGGE VIDTYPYFV SGVLHLISSA VLGFGGVYHS LVGPETLEES FPFFGYVWKD KNKMTTILGI HLIVLGLGAW LLVWKAMYFG G IYDTWAPG GGDVRIITNP TVSPGVIFGY LFRSPFGGDG WIVSVDNMED VIGGHIWIGT LCIFGGIWHI LTKPWAWARR AF VWSGEAY LSYSLGAISI MGFTACCMAW FNTTAYPSEF YGPTGPEASQ SQTFTFLVRD QRLGANVASA QGPTGLGKYL MRS PTGEII FGGETMRFWD FRGPWLEPLR GPNGLDLNKL KNDIQPWQER RAAEYMTHAP LGSLNSVGGV ATEINAVNYV SPRS WLATS HFCLGFFFFV GHLWHAGRAR AAAAGFEKGI DRDNEPVLSM RPL

UniProtKB: Photosystem II CP43 reaction center protein

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Macromolecule #8: Photosystem II D2 protein

MacromoleculeName: Photosystem II D2 protein / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 38.459945 KDa
SequenceString: KRGWFDVVDD WLRRDRFVFV GWSGLLLFPT AYLALGGWFT GTTFVTSWYT HGLASSYLEG CNFLTAAVST PANSMGHSLL FLWGPEAQG DFTRWCQLGG LWTFVALHGS FSLIGFMLRQ FEIARSVKIR PYNAIAFSAP ISVFVSVFLI YPLGQSGWFF A PSFGVAAI ...String:
KRGWFDVVDD WLRRDRFVFV GWSGLLLFPT AYLALGGWFT GTTFVTSWYT HGLASSYLEG CNFLTAAVST PANSMGHSLL FLWGPEAQG DFTRWCQLGG LWTFVALHGS FSLIGFMLRQ FEIARSVKIR PYNAIAFSAP ISVFVSVFLI YPLGQSGWFF A PSFGVAAI FRFILFFQGF HNWTLNPFHM MGVAGVLGAA LLCAIHGATV ENTLFEDGDG ANTFRAFNPT QAEETYSMVT AN RFWSQIF GVAFSNKRWL HFFMLFVPVT GLWMSAIGVV GLALNLRAYD FVSQEIRAAE DPEFETFYTK NILLNEGIRA WMA AQDQPH EKLVFPEEVL PRGNAL

UniProtKB: Photosystem II D2 protein

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Macromolecule #9: Cytochrome b559 subunit alpha

MacromoleculeName: Cytochrome b559 subunit alpha / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 9.204327 KDa
SequenceString:
SGATGERPFS DILTSIRYWV IHSITIPSLF IAGWLFVSTG LAYDVFGSPR PNEYFTEDRQ ETPLITDRFN ALEQVKKLSE V

UniProtKB: Cytochrome b559 subunit alpha

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Macromolecule #10: Cytochrome b559 subunit beta

MacromoleculeName: Cytochrome b559 subunit beta / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 4.262068 KDa
SequenceString:
KSYTYPIFTV RWLAVHALAV PTVFFLGAIT AMQFIQR

UniProtKB: Cytochrome b559 subunit beta

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Macromolecule #11: Photosystem II reaction center protein H

MacromoleculeName: Photosystem II reaction center protein H / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 7.223423 KDa
SequenceString:
DTGISTPLGT LLKPLNSEYG KVAPGWGTTV LMGIFMALFA VFLVIILEIY NSSVLLDDVT MSWESLA

UniProtKB: Photosystem II reaction center protein H

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Macromolecule #12: Photosystem II reaction center protein I

MacromoleculeName: Photosystem II reaction center protein I / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 3.871545 KDa
SequenceString:
MLTLKIFVYT VVTFFVSLFI FGFLSNDPGR NPGQ

UniProtKB: Photosystem II reaction center protein I

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Macromolecule #13: Photosystem II reaction center protein J

MacromoleculeName: Photosystem II reaction center protein J / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 3.813468 KDa
SequenceString:
GTTGRIPLWL VGTVAGVAVI ALVGIFFYGS YVGLGSSL

UniProtKB: Photosystem II reaction center protein J

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Macromolecule #14: Photosystem II reaction center protein K

MacromoleculeName: Photosystem II reaction center protein K / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 4.139958 KDa
SequenceString:
KLPEAYAPFD PIVDVLPVIP VLFLLLALVW QASVSFR

UniProtKB: Photosystem II reaction center protein K

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Macromolecule #15: Photosystem II reaction center protein L

MacromoleculeName: Photosystem II reaction center protein L / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 4.259966 KDa
SequenceString:
AKPNPNKQSV ELNRTSLYWG LLLIFVLAVL FSSYIFN

UniProtKB: Photosystem II reaction center protein L

+
Macromolecule #16: Photosystem II reaction center protein M

MacromoleculeName: Photosystem II reaction center protein M / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 5.621872 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)MEVN ILGVIATALF IIIPTSFLLI LYVKTASQ

UniProtKB: Photosystem II reaction center protein M

+
Macromolecule #17: Chloroplast oxygen-evolving enhancer protein 1

MacromoleculeName: Chloroplast oxygen-evolving enhancer protein 1 / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 25.449529 KDa
SequenceString: LTFDQLQGLT YLQVKGTGIA NTCPTLDSGS SNVKDLKPGT YKLGKFCMEP TSFTVKEESQ FKGGDAEFVP TKLMTRLTYT LDEMSGQFK VDGSGNVELK ETDGIDYAPV TVQLPGGERV PFLFSVKELD AKGNADGFGG EFVVPSYRGA TFLDPKGRGA A TGYDTAVA ...String:
LTFDQLQGLT YLQVKGTGIA NTCPTLDSGS SNVKDLKPGT YKLGKFCMEP TSFTVKEESQ FKGGDAEFVP TKLMTRLTYT LDEMSGQFK VDGSGNVELK ETDGIDYAPV TVQLPGGERV PFLFSVKELD AKGNADGFGG EFVVPSYRGA TFLDPKGRGA A TGYDTAVA LPAAGDDEEY LKENIKSAGA MKGQAVFSIA KVDAATGEIA GVFESVQPSD TDLGAKAPKD VKITGLWYAQ LS

UniProtKB: Chloroplast oxygen-evolving enhancer protein 1

+
Macromolecule #18: Chlorophyll a b binding CP29

MacromoleculeName: Chlorophyll a b binding CP29 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 25.803734 KDa
SequenceString: RSGGAGYRQY SGDALWLPNT ERPAWLDGSL PGDRGFDPLG LSRPAEYLQI DLDELDQNAA VNKAGGVVGA FTPVADQVST DSLAPYSEV FGLQRFRECE LIHGRWAMLA CLGALVQEGV TGDSWVAAQT LVYNQPQYAG IDLPFDIYTL AVLNSVLMGG V ELFRNSEL ...String:
RSGGAGYRQY SGDALWLPNT ERPAWLDGSL PGDRGFDPLG LSRPAEYLQI DLDELDQNAA VNKAGGVVGA FTPVADQVST DSLAPYSEV FGLQRFRECE LIHGRWAMLA CLGALVQEGV TGDSWVAAQT LVYNQPQYAG IDLPFDIYTL AVLNSVLMGG V ELFRNSEL DPERR(CSU)YPGG AFDPLNLASD DSERTQKLRE AEIKHARLAM VSFLGYTVQA WYTGEGALGS LNKFANGF

+
Macromolecule #19: Chlorophyll a b-binding CP26

MacromoleculeName: Chlorophyll a b-binding CP26 / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 27.369807 KDa
SequenceString: VSRSGGWLGS DSQNINLDKW YGPDRVLYLP GGLLARDEIN PVLNGTLPGD YGYDPLGLAK DAETLAKYRA NELLHARWAM LAAAGAIIP EGLAANGADV KGATWFETGA AMLNGGTLNW FAVPFVNFNN PLPLFAVVAI NVALMAAAEN YRRTEDGPAG Y APGVGKFD ...String:
VSRSGGWLGS DSQNINLDKW YGPDRVLYLP GGLLARDEIN PVLNGTLPGD YGYDPLGLAK DAETLAKYRA NELLHARWAM LAAAGAIIP EGLAANGADV KGATWFETGA AMLNGGTLNW FAVPFVNFNN PLPLFAVVAI NVALMAAAEN YRRTEDGPAG Y APGVGKFD ESVYSNMDNL YPGGPFDPLG LADDPEVLAE LKVKEIKNGR LAMVSFLGFA VQAAVTGEGP YANWSKHVAD PF GYNLLTI LSSEDRAAVL

+
Macromolecule #20: Photosystem II reaction center protein T

MacromoleculeName: Photosystem II reaction center protein T / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 3.47622 KDa
SequenceString:
MEALVYTFLL VGTLGIIFFA IFFREPPRIV

UniProtKB: Photosystem II reaction center protein T

+
Macromolecule #21: Photosystem II reaction center protein Ycf12

MacromoleculeName: Photosystem II reaction center protein Ycf12 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 3.31901 KDa
SequenceString:
NLEIVLQLTA LLFIVAAGPL VIVLLSSRGG NL

UniProtKB: Photosystem II reaction center protein Ycf12

+
Macromolecule #22: Photosystem II reaction center W protein

MacromoleculeName: Photosystem II reaction center W protein / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 6.62335 KDa
SequenceString:
LVDDRLAGEG TGKILGINDP SLFWAITIVF TIVWGVFYVS TREIDAASDR ENDDDFGLTL

+
Macromolecule #23: Photosystem II reaction center protein X

MacromoleculeName: Photosystem II reaction center protein X / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 3.526028 KDa
SequenceString:
AATPSLNNLI GSVVAGGLVL AAIAGAITAV SNFDSIR

+
Macromolecule #24: Multifunctional fusion protein

MacromoleculeName: Multifunctional fusion protein / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO / EC number: diacylglycerol kinase (ATP)
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 23.863996 KDa
SequenceString: VWYGPDRPKF LGPFSDGLVP AYLTGEFPGD YGWDTSGLSA DPETFKKYRT IEVIHARWAM LGALGCIFPE VLEKYSGVQF GEAVWFKAG AQIFADGGLN YLGNSGLVHA QSIVATLVVQ VLLMGAVEIY RANGEGPNGF GEGQDTLYPG GAFDPLGLAD D PDTLAELK ...String:
VWYGPDRPKF LGPFSDGLVP AYLTGEFPGD YGWDTSGLSA DPETFKKYRT IEVIHARWAM LGALGCIFPE VLEKYSGVQF GEAVWFKAG AQIFADGGLN YLGNSGLVHA QSIVATLVVQ VLLMGAVEIY RANGEGPNGF GEGQDTLYPG GAFDPLGLAD D PDTLAELK VKEIKNGRLA MLSMLGFFVQ AIVTGKGPLD NLLQHLEEPG EFNFYALYAN SLA

UniProtKB: Multifunctional fusion protein

+
Macromolecule #25: Photosystem II reaction center protein Z

MacromoleculeName: Photosystem II reaction center protein Z / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 6.727147 KDa
SequenceString:
MLLIFQLALF AFIVVSFLLV VGVPVVLATP EGWAENKGTV FSGIGIWFLL VFLVGILNSF VV

UniProtKB: Photosystem II reaction center protein Z

+
Macromolecule #26: Chloroplast oxygen-evolving enhancer protein 3

MacromoleculeName: Chloroplast oxygen-evolving enhancer protein 3 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 15.948137 KDa
SequenceString:
LDLFDDRKAR QAGFDIIYEA RDLDLPQGVR DGLAQARENL DVAKARIKEA EKRIDADLDA YIAKAYWTEG RNELRRQVGT LRFDLNAVA DTLPKAAKKE AQAAKNEFLA AVEALDLQLR KKNGDKAAAA LADTKAKLDA VIAKL

UniProtKB: Chloroplast oxygen-evolving enhancer protein 3

+
Macromolecule #27: Chloroplast PsbY

MacromoleculeName: Chloroplast PsbY / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 3.697331 KDa
SequenceString:
DNRFGTIALL AVPVIGWVLF NILGPLQNQL NAMS

UniProtKB: Chloroplast PsbY

+
Macromolecule #28: Chloroplast photosystem II 10 kDa protein

MacromoleculeName: Chloroplast photosystem II 10 kDa protein / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 11.657287 KDa
SequenceString:
KIDISKQGLN SIQNETVKLN LMGKSKTMES KDWVDPQGRK GKGYGVYRFA NKYGANVDGY SPIYTPDTWS ESGDSYKLGT KGLIAWAGL IVVLLGVGIN LVISTSALG

UniProtKB: Chloroplast photosystem II 10 kDa protein

+
Macromolecule #29: Photosystem II oxygen evolving enhancer 2

MacromoleculeName: Photosystem II oxygen evolving enhancer 2 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlorella ohadii (plant)
Molecular weightTheoretical: 20.416791 KDa
SequenceString:
AYGDAARVFA GNITNKSGFI PYAGEGFALL IPSKWNPSKE QDFPGVVLRY EDNGDAVNNL VVLVQKVGKN NIDELGSPDK FLQDNAYLF GESAAFTGET LSEGGFLPNK VAAASVLDVQ EATDKKGKKY YKYEVLTRSA DGDEGGRHQL IAATVSNGNL Y LIKVQCGD KRWFKGAKRE AIGAWDSFTV

+
Macromolecule #30: CHLOROPHYLL B

MacromoleculeName: CHLOROPHYLL B / type: ligand / ID: 30 / Number of copies: 122 / Formula: CHL
Molecular weightTheoretical: 907.472 Da
Chemical component information

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

+
Macromolecule #31: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 31 / Number of copies: 270 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

+
Macromolecule #32: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

MacromoleculeName: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 32 / Number of copies: 36 / Formula: LUT
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

+
Macromolecule #33: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 33 / Number of copies: 44 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

+
Macromolecule #34: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 34 / Number of copies: 86 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

+
Macromolecule #35: (3R)-beta,beta-caroten-3-ol

MacromoleculeName: (3R)-beta,beta-caroten-3-ol / type: ligand / ID: 35 / Number of copies: 6 / Formula: RRX
Molecular weightTheoretical: 552.872 Da
Chemical component information

ChemComp-RRX:
(3R)-beta,beta-caroten-3-ol / Β-Cryptoxanthin

+
Macromolecule #36: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...

MacromoleculeName: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
type: ligand / ID: 36 / Number of copies: 22 / Formula: NEX
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

+
Macromolecule #37: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...

MacromoleculeName: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 37 / Number of copies: 8 / Formula: XAT
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

+
Macromolecule #38: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 38 / Number of copies: 24 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

+
Macromolecule #39: CA-MN4-O5 CLUSTER

MacromoleculeName: CA-MN4-O5 CLUSTER / type: ligand / ID: 39 / Number of copies: 2 / Formula: OEX
Molecular weightTheoretical: 339.827 Da
Chemical component information

ChemComp-OEX:
CA-MN4-O5 CLUSTER

+
Macromolecule #40: FE (II) ION

MacromoleculeName: FE (II) ION / type: ligand / ID: 40 / Number of copies: 2 / Formula: FE2
Molecular weightTheoretical: 55.845 Da

+
Macromolecule #41: CHLORIDE ION

MacromoleculeName: CHLORIDE ION / type: ligand / ID: 41 / Number of copies: 4 / Formula: CL
Molecular weightTheoretical: 35.453 Da

+
Macromolecule #42: PHEOPHYTIN A

MacromoleculeName: PHEOPHYTIN A / type: ligand / ID: 42 / Number of copies: 4 / Formula: PHO
Molecular weightTheoretical: 871.2 Da
Chemical component information

ChemComp-PHO:
PHEOPHYTIN A / Pheophytin

+
Macromolecule #43: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 43 / Number of copies: 22 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

+
Macromolecule #44: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...

MacromoleculeName: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
type: ligand / ID: 44 / Number of copies: 4 / Formula: PL9
Molecular weightTheoretical: 749.201 Da
Chemical component information

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / Plastoquinone

+
Macromolecule #45: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 45 / Number of copies: 8 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

+
Macromolecule #46: BICARBONATE ION

MacromoleculeName: BICARBONATE ION / type: ligand / ID: 46 / Number of copies: 2 / Formula: BCT
Molecular weightTheoretical: 61.017 Da
Chemical component information

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM / Bicarbonate

+
Macromolecule #47: PROTOPORPHYRIN IX CONTAINING FE

MacromoleculeName: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 47 / Number of copies: 2 / Formula: HEM
Molecular weightTheoretical: 616.487 Da
Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

+
Macromolecule #48: DODECYL-ALPHA-D-MALTOSIDE

MacromoleculeName: DODECYL-ALPHA-D-MALTOSIDE / type: ligand / ID: 48 / Number of copies: 4 / Formula: LMU
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

+
Macromolecule #49: water

MacromoleculeName: water / type: ligand / ID: 49 / Number of copies: 224 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration4 mg/mL
BufferpH: 6 / Details: 20 mM MES NaOH pH 6 0.1% alfaDM
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 12 / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 40 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 293 K / Instrument: LEICA EM GP

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number real images: 21341 / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 734768
Startup modelType of model: OTHER
Initial angle assignmentType: OTHER
Final 3D classificationNumber classes: 10 / Avg.num./class: 12000
Final reconstructionNumber classes used: 8 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.73 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 94893
FSC plot (resolution estimation)

+
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