EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Benchmarking the ideal sample thickness in cryo-EM.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 49, Year 2021
掲載日	2021年12月7日
著者	Michael W Martynowycz / Max T B Clabbers / Johan Unge / Johan Hattne / Tamir Gonen /
PubMed 要旨	The relationship between sample thickness and quality of data obtained is investigated by microcrystal electron diffraction (MicroED). Several electron microscopy (EM) grids containing proteinase K ...The relationship between sample thickness and quality of data obtained is investigated by microcrystal electron diffraction (MicroED). Several electron microscopy (EM) grids containing proteinase K microcrystals of similar sizes from the same crystallization batch were prepared. Each grid was transferred into a focused ion beam and a scanning electron microscope in which the crystals were then systematically thinned into lamellae between 95- and 1,650-nm thick. MicroED data were collected at either 120-, 200-, or 300-kV accelerating voltages. Lamellae thicknesses were expressed in multiples of the corresponding inelastic mean free path to allow the results from different acceleration voltages to be compared. The quality of the data and subsequently determined structures were assessed using standard crystallographic measures. Structures were reliably determined with similar quality from crystalline lamellae up to twice the inelastic mean free path. Lower resolution diffraction was observed at three times the mean free path for all three accelerating voltages, but the data quality was insufficient to yield structures. Finally, no coherent diffraction was observed from lamellae thicker than four times the calculated inelastic mean free path. This study benchmarks the ideal specimen thickness with implications for all cryo-EM methods.
リンク	Proc Natl Acad Sci U S A / PubMed:34873060 / PubMed Central
手法	EM (電子線結晶学)
解像度	1.85 - 2.9 Å
構造データ	25456 7svy EMDB-25456, PDB-7svy: MicroED structure of proteinase K from a 130 nm thick lamella measured at 120 kV 手法: EM (電子線結晶学) 25457 7svz EMDB-25457, PDB-7svz: MicroED structure of proteinase K from a 200 nm thick lamella measured at 120 kV 手法: EM (電子線結晶学) 25458 7sw0 EMDB-25458, PDB-7sw0: MicroED structure of proteinase K from a 325 nm thick lamella measured at 120 kV 手法: EM (電子線結晶学) 25459 7sw1 EMDB-25459, PDB-7sw1: MicroED structure of proteinase K from a 115 nm thick lamella measured at 200 kV 手法: EM (電子線結晶学) 25460 7sw2 EMDB-25460, PDB-7sw2: MicroED structure of proteinase K from a 130 nm thick lamella measured at 200 kV 手法: EM (電子線結晶学) 25461 7sw3 EMDB-25461, PDB-7sw3: MicroED structure of proteinase K from a 95 nm thick lamella measured at 200 kV 手法: EM (電子線結晶学) 25462 7sw4 EMDB-25462, PDB-7sw4: MicroED structure of proteinase K from a 540 nm thick lamella measured at 200 kV 手法: EM (電子線結晶学) 25463 7sw5 EMDB-25463, PDB-7sw5: MicroED structure of proteinase K from a 460 nm thick lamella measured at 200 kV 手法: EM (電子線結晶学) 25464 7sw6 EMDB-25464, PDB-7sw6: MicroED structure of proteinase K from a 260 nm thick lamella measured at 200 kV 手法: EM (電子線結晶学) 25465 7sw7 EMDB-25465, PDB-7sw7: MicroED structure of proteinase K from a 530 nm thick lamella measured at 200 kV 手法: EM (電子線結晶学) 25466 7sw8 EMDB-25466, PDB-7sw8: MicroED structure of proteinase K from a 150 nm thick lamella measured at 300 kV 手法: EM (電子線結晶学) 25467 7sw9 EMDB-25467, PDB-7sw9: MicroED structure of proteinase K from a 170 nm thick lamella measured at 300 kV 手法: EM (電子線結晶学) 25468 7swa EMDB-25468, PDB-7swa: MicroED structure of proteinase K from a 320 nm thick lamella measured at 300 kV 手法: EM (電子線結晶学) 25469 7swb EMDB-25469, PDB-7swb: MicroED structure of proteinase K from a 360 nm thick lamella measured at 300 kV 手法: EM (電子線結晶学) 25470 7swc EMDB-25470, PDB-7swc: MicroED structure of proteinase K from a 550 nm thick lamella measured at 300 kV 手法: EM (電子線結晶学)
化合物	ChemComp-HOH: WATER / 水
由来	parengyodontium album (菌類)
キーワード	HYDROLASE (加水分解酵素) / Serine protease (セリンプロテアーゼ)