Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes.
Journal, issue, pages	EMBO J, Vol. 40, Issue 1, Page e105179, Year 2021
Publish date	Jan 4, 2021
Authors	Hanna Kratzat / Timur Mackens-Kiani / Michael Ameismeier / Mia Potocnjak / Jingdong Cheng / Estelle Dacheux / Abdelkader Namane / Otto Berninghausen / Franz Herzog / Micheline Fromont-Racine / Thomas Becker / Roland Beckmann /
PubMed Abstract	In eukaryotic translation, termination and ribosome recycling phases are linked to subsequent initiation of a new round of translation by persistence of several factors at ribosomal sub-complexes. ...In eukaryotic translation, termination and ribosome recycling phases are linked to subsequent initiation of a new round of translation by persistence of several factors at ribosomal sub-complexes. These comprise/include the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of the next 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S, but also with later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide-binding domains, while interacting with the N-terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C-terminus providing a structural explanation for its antagonistic role with respect to mRNA binding. Overall, the native human samples provide a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and the eIF2 ternary complex containing the initiator tRNA.
External links	EMBO J / PubMed:33289941 / PubMed Central
Methods	EM (single particle)
Resolution	3.0 - 6.2 Å
Structure data	11160 6zce EMDB-11160, PDB-6zce: Structure of a yeast ABCE1-bound 43S pre-initiation complex Method: EM (single particle) / Resolution: 5.3 Å 11439 6zu9 EMDB-11439, PDB-6zu9: Structure of a yeast ABCE1-bound 48S initiation complex Method: EM (single particle) / Resolution: 6.2 Å 11458 6zvj EMDB-11458, PDB-6zvj: Structure of a human ABCE1-bound 43S pre-initiation complex - State II Method: EM (single particle) / Resolution: 3.8 Å 11602 7a09 EMDB-11602, PDB-7a09: Structure of a human ABCE1-bound 43S pre-initiation complex - State III Method: EM (single particle) / Resolution: 3.5 Å 11608 7a1g EMDB-11608, PDB-7a1g: Structure of a crosslinked yeast ABCE1-bound 43S pre-initiation complex Method: EM (single particle) / Resolution: 3.0 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM / Adenosine diphosphate ChemComp-MG: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate ChemComp-SF4: IRON/SULFUR CLUSTER / Iron–sulfur cluster ChemComp-MET: METHIONINE / Methionine ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate
Source	saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast) Baker's yeast (brewer's yeast) saccharomyces cerevisiae s288c (yeast) homo sapiens (human) Human (human)
Keywords	RIBOSOME / Translation / Initiation / Ribosome Recycling / ABC Proteins