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TitleCryo-electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B.
Journal, issue, pagesScience, Vol. 366, Issue 6471, Page 1372-1375, Year 2019
Publish dateDec 13, 2019
AuthorsAna S Ramírez / Julia Kowal / Kaspar P Locher /
PubMed AbstractOligosaccharyltransferase (OST) catalyzes the transfer of a high-mannose glycan onto secretory proteins in the endoplasmic reticulum. Mammals express two distinct OST complexes that act in a ...Oligosaccharyltransferase (OST) catalyzes the transfer of a high-mannose glycan onto secretory proteins in the endoplasmic reticulum. Mammals express two distinct OST complexes that act in a cotranslational (OST-A) or posttranslocational (OST-B) manner. Here, we present high-resolution cryo-electron microscopy structures of human OST-A and OST-B. Although they have similar overall architectures, structural differences in the catalytic subunits STT3A and STT3B facilitate contacts to distinct OST subunits, DC2 in OST-A and MAGT1 in OST-B. In OST-A, interactions with TMEM258 and STT3A allow ribophorin-I to form a four-helix bundle that can bind to a translating ribosome, whereas the equivalent region is disordered in OST-B. We observed an acceptor peptide and dolichylphosphate bound to STT3B, but only dolichylphosphate in STT3A, suggesting distinct affinities of the two OST complexes for protein substrates.
External linksScience / PubMed:31831667
MethodsEM (single particle)
Resolution3.5 Å
Structure data

10110

6s7o

EMDB-10110, PDB-6s7o:
Cryo-EM structure of human oligosaccharyltransferase complex OST-A
Method: EM (single particle) / Resolution: 3.5 Å

10112

6s7t

EMDB-10112, PDB-6s7t:
Cryo-EM structure of human oligosaccharyltransferase complex OST-B
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-KZB:
(2~{S},3~{R},4~{R},5~{S},6~{S})-2-(hydroxymethyl)-6-[(1~{S},2~{R},3~{R},4~{R},5'~{S},6~{S},7~{R},8~{S},9~{R},12~{R},13~{R},15~{S},16~{S},18~{R})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol

ChemComp-EGY:
(4R,7R)-4-hydroxy-N,N,N-trimethyl-4,9-dioxo-7-[(undecanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphadocosan-1-aminium / phospholipid*YM

ChemComp-MG:
Unknown entry

ChemComp-KZE:
[(3~{R},6~{Z},10~{Z},14~{Z},18~{Z})-3,7,11,15,19,23-hexamethyltetracosa-6,10,14,18,22-pentaenyl] dihydrogen phosphate

ChemComp-0K3:
(2Z,6Z,10Z,14Z,18Z,22Z,26Z)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl dihydrogen phosphate

Source
  • homo sapiens (human)
  • Human (human)
KeywordsTRANSFERASE / N-glycosylation / Oligosaccharyltransferase / OSTA / OSTB

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