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TitleCardiac muscle thin filament structures reveal calcium regulatory mechanism.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 153, Year 2020
Publish dateJan 9, 2020
AuthorsYurika Yamada / Keiichi Namba / Takashi Fujii /
PubMed AbstractContraction of striated muscles is driven by cyclic interactions of myosin head projecting from the thick filament with actin filament and is regulated by Ca released from sarcoplasmic reticulum. ...Contraction of striated muscles is driven by cyclic interactions of myosin head projecting from the thick filament with actin filament and is regulated by Ca released from sarcoplasmic reticulum. Muscle thin filament consists of actin, tropomyosin and troponin, and Ca binding to troponin triggers conformational changes of troponin and tropomyosin to allow actin-myosin interactions. However, the structural changes involved in this regulatory mechanism remain unknown. Here we report the structures of human cardiac muscle thin filament in the absence and presence of Ca by electron cryomicroscopy. Molecular models in the two states built based on available crystal structures reveal the structures of a C-terminal region of troponin I and an N-terminal region of troponin T in complex with the head-to-tail junction of tropomyosin together with the troponin core on actin filament. Structural changes of the thin filament upon Ca binding now reveal the mechanism of Ca regulation of muscle contraction.
External linksNat Commun / PubMed:31919429 / PubMed Central
MethodsEM (single particle)
Resolution4.8 - 6.6 Å
Structure data

0728

6kn7

EMDB-0728, PDB-6kn7:
Structure of human cardiac thin filament in the calcium free state
Method: EM (single particle) / Resolution: 6.6 Å

0729

6kn8

EMDB-0729, PDB-6kn8:
Structure of human cardiac thin filament in the calcium bound state
Method: EM (single particle) / Resolution: 4.8 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • oryctolagus cuniculus (rabbit)
  • homo sapiens (human)
KeywordsCONTRACTILE PROTEIN/ACTIN BINDING PROTEIN / Troponin / Tropomyosin / Actin / Thin filement / Muscle / CONTRACTILE PROTEIN-ACTIN BINDING PROTEIN complex

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