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Structure paper

TitleStructure of eEF3 and the mechanism of transfer RNA release from the E-site.
Journal, issue, pagesNature, Vol. 443, Issue 7112, Page 663-668, Year 2006
Publish dateOct 12, 2006
AuthorsChristian B F Andersen / Thomas Becker / Michael Blau / Monika Anand / Mario Halic / Bharvi Balar / Thorsten Mielke / Thomas Boesen / Jan Skov Pedersen / Christian M T Spahn / Terri Goss Kinzy / Gregers R Andersen / Roland Beckmann /
PubMed AbstractElongation factor eEF3 is an ATPase that, in addition to the two canonical factors eEF1A and eEF2, serves an essential function in the translation cycle of fungi. eEF3 is required for the binding of ...Elongation factor eEF3 is an ATPase that, in addition to the two canonical factors eEF1A and eEF2, serves an essential function in the translation cycle of fungi. eEF3 is required for the binding of the aminoacyl-tRNA-eEF1A-GTP ternary complex to the ribosomal A-site and has been suggested to facilitate the clearance of deacyl-tRNA from the E-site. Here we present the crystal structure of Saccharomyces cerevisiae eEF3, showing that it consists of an amino-terminal HEAT repeat domain, followed by a four-helix bundle and two ABC-type ATPase domains, with a chromodomain inserted in ABC2. Moreover, we present the cryo-electron microscopy structure of the ATP-bound form of eEF3 in complex with the post-translocational-state 80S ribosome from yeast. eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain likely to stabilize the ribosomal L1 stalk in an open conformation, thus allowing tRNA release.
External linksNature / PubMed:16929303
MethodsEM (single particle) / X-ray diffraction
Resolution2.4 - 9.9 Å
Structure data

1233

2ix8

EMDB-1233: Structure of eEF3 and the mechanism of transfer RNA release from the E-site.
PDB-2ix8: MODEL FOR EEF3 BOUND TO AN 80S RIBOSOME
Method: EM (single particle) / Resolution: 9.9 Å

PDB-2iw3:
Elongation Factor 3 in complex with ADP
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-2iwh:
Structure of yeast Elongation Factor 3 in complex with ADPNP
Method: X-RAY DIFFRACTION / Resolution: 3.0 Å

PDB-2ix3:
Structure of yeast Elongation Factor 3
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSLATION / ACETYLATION / ATP-BINDING / ELONGATION FACTOR / PROTEIN BIOSYNTHESIS / NUCLEOTIDE-BINDING / PHOSPHORYLATION / RNA-BINDING / RRNA-BINDING

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