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TitleMultimodal microtubule binding by the Ndc80 kinetochore complex.
Journal, issue, pagesNat Struct Mol Biol, Vol. 19, Issue 11, Page 1161-1167, Year 2012
Publish dateOct 21, 2012
AuthorsGregory M Alushin / Vivek Musinipally / Daniel Matson / John Tooley / P Todd Stukenberg / Eva Nogales /
PubMed AbstractThe Ndc80 complex is a key site of kinetochore-microtubule attachment during cell division. The human complex engages microtubules with a globular 'head' formed by tandem calponin-homology domains ...The Ndc80 complex is a key site of kinetochore-microtubule attachment during cell division. The human complex engages microtubules with a globular 'head' formed by tandem calponin-homology domains and an 80-amino-acid unstructured 'tail' that contains sites of phosphoregulation by the Aurora B kinase. Using biochemical, cell biological and electron microscopy analyses, we dissected the roles of the tail in binding of microtubules and mediation of cooperative interactions between Ndc80 complexes. Two segments of the tail that contain Aurora B phosphorylation sites become ordered at interfaces; one with tubulin and the second with an adjacent Ndc80 head on the microtubule surface, forming interactions that are disrupted by phosphorylation. We propose a model in which Ndc80's interaction with either growing or shrinking microtubule ends can be tuned by the phosphorylation state of its tail.
External linksNat Struct Mol Biol / PubMed:23085714 / PubMed Central
MethodsEM (helical sym.)
Resolution7.9 - 13.0 Å
Structure data

EMDB-5489:
Improved Reconstruction of the Human Ndc80 Bonsai Decorated Microtubule
Method: EM (helical sym.) / Resolution: 7.9 Å

EMDB-5490:
Reconstruction of the Wild-type Ndc80 Bonsai Decorated Microtubule on CCD for Difference Map Calculation
Method: EM (helical sym.) / Resolution: 12.8 Å

EMDB-5491:
Reconstruction of the Ndc80 Bonsai 4D Mutant Decorated Microtubule on CCD for Difference Map Calculation
Method: EM (helical sym.) / Resolution: 12.1 Å

EMDB-5492:
Reconstruction of the Ndc80 Bonsai Delta 40 Decorated Microtubule on CCD for Difference Map Calculation
Method: EM (helical sym.) / Resolution: 13.0 Å

EMDB-5493:
Reconstruction of the Microtubule Alone on CCD for Difference Map Calculation
Method: EM (helical sym.) / Resolution: 10.8 Å

Source
  • Bos taurus (cattle)
  • Homo sapiens (human)

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