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TitleCharacterization of Fully Recombinant Human 20S and 20S-PA200 Proteasome Complexes.
Journal, issue, pagesMol Cell, Vol. 76, Issue 1, Page 138-147.e5, Year 2019
Publish dateOct 3, 2019
AuthorsAna Toste Rêgo / Paula C A da Fonseca /
PubMed AbstractProteasomes are essential in all eukaryotic cells. However, their function and regulation remain considerably elusive, particularly those of less abundant variants. We demonstrate the human 20S ...Proteasomes are essential in all eukaryotic cells. However, their function and regulation remain considerably elusive, particularly those of less abundant variants. We demonstrate the human 20S proteasome recombinant assembly and confirmed the recombinant complex integrity biochemically and with a 2.6 Å resolution cryo-EM map. To assess its competence to form higher-order assemblies, we prepared and analyzed recombinant human 20S-PA200, a poorly characterized nuclear complex. Its 3.0 Å resolution cryo-EM structure reveals the PA200 unique architecture; the details of its intricate interactions with the proteasome, resulting in unparalleled proteasome α ring rearrangements; and the molecular basis for PA200 allosteric modulation of the proteasome active sites. Non-protein cryo-EM densities could be assigned to PA200-bound inositol phosphates, and we speculate regarding their functional role. Here we open extensive opportunities to study the fundamental properties of the diverse and distinct eukaryotic proteasome variants and to improve proteasome targeting under different therapeutic conditions.
External linksMol Cell / PubMed:31473102 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.0 Å
Structure data

4860

6rey

EMDB-4860, PDB-6rey:
Human 20S-PA200 Proteasome Complex
Method: EM (single particle) / Resolution: 3.0 Å

4877

6rgq

EMDB-4877, PDB-6rgq:
Human 20S Proteasome
Method: EM (single particle) / Resolution: 2.6 Å

Chemicals

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

ChemComp-K0W:
[(1~{S},2~{R},3~{R},4~{S},5~{S},6~{R})-2-[oxidanyl(phosphonooxy)phosphoryl]oxy-3,4,5,6-tetraphosphonooxy-cyclohexyl] phosphono hydrogen phosphate

Source
  • homo sapiens (human)
KeywordsHYDROLASE / proteasome / PA200 / activator

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