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-Structure paper
タイトル | High-Resolution Cryo-Electron Microscopy Structure Determination of Tellurite-Resistance Protein A via 200 kV Transmission Electron Microscopy. |
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ジャーナル・号・ページ | Int J Mol Sci, Vol. 25, Issue 8, Year 2024 |
掲載日 | 2024年4月20日 |
著者 | Nhi L Tran / Skerdi Senko / Kyle W Lucier / Ashlyn C Farwell / Sabrina M Silva / Phat V Dip / Nicole Poweleit / Giovanna Scapin / Claudio Catalano / |
PubMed 要旨 | Membrane proteins constitute about 20% of the human proteome and play crucial roles in cellular functions. However, a complete understanding of their structure and function is limited by their ...Membrane proteins constitute about 20% of the human proteome and play crucial roles in cellular functions. However, a complete understanding of their structure and function is limited by their hydrophobic nature, which poses significant challenges in purification and stabilization. Detergents, essential in the isolation process, risk destabilizing or altering the proteins' native conformations, thus affecting stability and functionality. This study leverages single-particle cryo-electron microscopy to elucidate the structural nuances of membrane proteins, focusing on the SLAC1 bacterial homolog from (TehA) purified with diverse detergents, including n-dodecyl β-D-maltopyranoside (DDM), glycodiosgenin (GDN), β-D-octyl-glucoside (OG), and lauryl maltose neopentyl glycol (LMNG). This research not only contributes to the understanding of membrane protein structures but also addresses detergent effects on protein purification. By showcasing that the overall structural integrity of the channel is preserved, our study underscores the intricate interplay between proteins and detergents, offering insightful implications for drug design and membrane biology. |
リンク | Int J Mol Sci / PubMed:38674110 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.9 - 3.2 Å |
構造データ | EMDB-43246, PDB-8vi2: EMDB-43247, PDB-8vi3: EMDB-43248, PDB-8vi4: EMDB-43249, PDB-8vi5: |
由来 |
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キーワード | MEMBRANE PROTEIN (膜タンパク質) / ANION CHANNEL (イオンチャネル) / ALPHA HELICAL INTEGRAL MEMBRANE PROTEIN |