EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Eliciting a single amino acid change by vaccination generates antibody protection against group 1 and group 2 influenza A viruses.
ジャーナル・号・ページ	Immunity, Year 2024
掲載日	2024年4月18日
著者	Rashmi Ray / Faez Amokrane Nait Mohamed / Daniel P Maurer / Jiachen Huang / Berk A Alpay / Larance Ronsard / Zhenfei Xie / Julianna Han / Monica Fernandez-Quintero / Quynh Anh Phan / Rebecca L Ursin / Mya Vu / Kathrin H Kirsch / Thavaleak Prum / Victoria C Rosado / Thalia Bracamonte-Moreno / Vintus Okonkwo / Julia Bals / Caitlin McCarthy / Usha Nair / Masaru Kanekiyo / Andrew B Ward / Aaron G Schmidt / Facundo D Batista / Daniel Lingwood /
PubMed 要旨	Broadly neutralizing antibodies (bnAbs) targeting the hemagglutinin (HA) stem of influenza A viruses (IAVs) tend to be effective against either group 1 or group 2 viral diversity. In rarer cases, ...Broadly neutralizing antibodies (bnAbs) targeting the hemagglutinin (HA) stem of influenza A viruses (IAVs) tend to be effective against either group 1 or group 2 viral diversity. In rarer cases, intergroup protective bnAbs can be generated by human antibody paratopes that accommodate the conserved glycan differences between the group 1 and group 2 stems. We applied germline-engaging nanoparticle immunogens to elicit a class of cross-group bnAbs from physiological precursor frequency within a humanized mouse model. Cross-group protection depended on the presence of the human bnAb precursors within the B cell repertoire, and the vaccine-expanded antibodies enriched for an N55T substitution in the CDRH2 loop, a hallmark of the bnAb class. Structurally, this single mutation introduced a flexible fulcrum to accommodate glycosylation differences and could alone enable cross-group protection. Thus, broad IAV immunity can be expanded from the germline repertoire via minimal antigenic input and an exceptionally simple antibody development pathway.
リンク	Immunity / PubMed:38670113
手法	EM (単粒子) / X線回折
解像度	2.7 - 4.02 Å
構造データ	42528 8ut3 EMDB-42528, PDB-8ut3: CryoEM structure of A/Perth/16/2009 H3 in complex with flu HA central stem VH1-18 antibody UCA6 手法: EM (単粒子) / 解像度: 2.8 Å 42529 8ut4 EMDB-42529, PDB-8ut4: CryoEM structure of A/Michigan/45/2015 H1 in complex with flu HA central stem VH1-18 antibody 09-1B12 手法: EM (単粒子) / 解像度: 3.3 Å 42530 8ut5 EMDB-42530, PDB-8ut5: CryoEM structure of A/Michigan/45/2015 H1 in complex with flu HA central stem VH1-18 antibody UCA6_N55T 手法: EM (単粒子) / 解像度: 3.5 Å 42531 8ut6 EMDB-42531, PDB-8ut6: CryoEM structure of A/Perth/16/2009 H3 in complex with polyclonal Fab from mice immunized with H3 stem nanoparticles-15 days post immunization 手法: EM (単粒子) / 解像度: 2.8 Å 42532 8ut7 EMDB-42532, PDB-8ut7: CryoEM structure of A/Perth/16/2009 H3 in complex with polyclonal Fab from mice immunized with H3 stem nanoparticles-28 days post immunization 手法: EM (単粒子) / 解像度: 2.7 Å 42533 8ut8 EMDB-42533, PDB-8ut8: CryoEM structure of A/Shanghai/1/2013 H7 in complex with polyclonal Fab from mice immunized with H7 stem nanoparticles-15 days post-immunization 手法: EM (単粒子) / 解像度: 3.2 Å 42534 8ut9 EMDB-42534, PDB-8ut9: CryoEM structure of A/Shanghai/1/2013 H7 in complex with polyclonal Fab from mice immunized with H7 stem nanoparticles-28 days post immunization 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-42535: CryoEM structure of A/Perth/16/2009 H3 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-42536: CryoEM map of A/Shanghai/1/2013 H7 HA 手法: EM (単粒子) / 解像度: 3.1 Å PDB-8uwa: VH1-18 QxxV class antibody 09-1B12 bound to A/Perth/16/2009 H3N2 hemagglutinin 手法: X-RAY DIFFRACTION / 解像度: 4.02 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	homo sapiens (ヒト) influenza a virus (A型インフルエンザウイルス) mus musculus (ハツカネズミ) influenza a virus (a/perth/16/2009(h3n2)) (A型インフルエンザウイルス)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / Flu Hemagglutinin / Central stem epitope / VH1-18 / VIRAL PROTEIN (ウイルスタンパク質) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性) / cryoEMPEM / antibody (抗体) / stem (ステム) / influenza (インフルエンザ) / hemagglutinin (ヘマグルチニン)