Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structures of membrane-bound dynamin in a post-hydrolysis state primed for membrane fission.
Journal, issue, pages	Dev Cell, Year 2024
Publish date	Apr 23, 2024
Authors	John R Jimah / Nidhi Kundu / Abigail E Stanton / Kem A Sochacki / Bertram Canagarajah / Lieza Chan / Marie-Paule Strub / Huaibin Wang / Justin W Taraska / Jenny E Hinshaw /
PubMed Abstract	Dynamin assembles as a helical polymer at the neck of budding endocytic vesicles, constricting the underlying membrane as it progresses through the GTPase cycle to sever vesicles from the ...Dynamin assembles as a helical polymer at the neck of budding endocytic vesicles, constricting the underlying membrane as it progresses through the GTPase cycle to sever vesicles from the plasma membrane. Although atomic models of the dynamin helical polymer bound to guanosine triphosphate (GTP) analogs define earlier stages of membrane constriction, there are no atomic models of the assembled state post-GTP hydrolysis. Here, we used cryo-EM methods to determine atomic structures of the dynamin helical polymer assembled on lipid tubules, akin to necks of budding endocytic vesicles, in a guanosine diphosphate (GDP)-bound, super-constricted state. In this state, dynamin is assembled as a 2-start helix with an inner lumen of 3.4 nm, primed for spontaneous fission. Additionally, by cryo-electron tomography, we trapped dynamin helical assemblies within HeLa cells using the GTPase-defective dynamin K44A mutant and observed diverse dynamin helices, demonstrating that dynamin can accommodate a range of assembled complexes in cells that likely precede membrane fission.
External links	Dev Cell / PubMed:38663399
Methods	EM (helical sym.)
Resolution	2.84 - 3.97 Å
Structure data	40861 8sxz EMDB-40861, PDB-8sxz: Cryo-EM of the GDP-bound human dynamin polymer assembled on the membrane in the super constricted state Method: EM (helical sym.) / Resolution: 3.26 Å 40901 8sz4 EMDB-40901, PDB-8sz4: Cryo-EM of the GDP-bound human dynamin polymer assembled on the membrane in the super constricted state showing the PH domain Method: EM (helical sym.) / Resolution: 2.86 Å 40902 8sz7 EMDB-40902, PDB-8sz7: Cryo-EM of the GDP-bound human dynamin polymer assembled on the membrane in the super constricted state showing the second PH domain Method: EM (helical sym.) / Resolution: 2.84 Å 40903 8sz8 EMDB-40903, PDB-8sz8: Cryo-EM of the GDP-bound human dynamin polymer assembled on the membrane in the super constricted state (full helix) Method: EM (helical sym.) / Resolution: 3.55 Å 40942 8t0k EMDB-40942, PDB-8t0k: Cryo-EM of the GDP-bound human dynamin (full-length) polymer assembled on the membrane in the super constricted state Method: EM (helical sym.) / Resolution: 3.58 Å 40946 8t0r EMDB-40946, PDB-8t0r: Cryo-EM of the GDP-bound human dynamin (full-length) polymer assembled on the membrane in the super constricted state (full helix) Method: EM (helical sym.) / Resolution: 3.97 Å
Chemicals	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM / Guanosine diphosphate ChemComp-MG*: Unknown entry
Source	homo sapiens (human)
Keywords	HYDROLASE / dynamin / membrane / fission / lipid / tubule / scission / endocytosis