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Structure paper

TitleStructural basis for sugar perception by gustatory receptors.
Journal, issue, pagesScience, Vol. 383, Issue 6685, Page eadj2609, Year 2024
Publish dateFeb 23, 2024
AuthorsDemin Ma / Meiqin Hu / Xiaotong Yang / Qiang Liu / Fan Ye / Weijie Cai / Yong Wang / Ximing Xu / Shenghai Chang / Ruiying Wang / Wei Yang / Sheng Ye / Nannan Su / Minrui Fan / Haoxing Xu / Jiangtao Guo /
PubMed AbstractInsects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste ...Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs.
External linksScience / PubMed:38305684
MethodsEM (single particle)
Resolution2.5 - 3.1 Å
Structure data

36410

8jm9

EMDB-36410, PDB-8jm9:
The cryo-EM structure of insect gustatory receptor Gr43a from Drosophila melanogaster
Method: EM (single particle) / Resolution: 2.6 Å

36411

8jma

EMDB-36411, PDB-8jma:
The cryo-EM structure of insect gustatory receptor Gr43a from Drosophila melanogaster in complex with fructose
Method: EM (single particle) / Resolution: 2.5 Å

36417

8jme

EMDB-36417, PDB-8jme:
The cryo-EM structure of insect gustatory receptor Gr64a from Drosophila melanogaster
Method: EM (single particle) / Resolution: 2.5 Å

36421

8jmh

EMDB-36421, PDB-8jmh:
The cryo-EM structure of insect gustatory receptor Gr64a from Drosophila melanogaster in complex with sucrose
Method: EM (single particle) / Resolution: 2.5 Å

36422

8jmi

EMDB-36422, PDB-8jmi:
The cryo-EM structure of insect gustatory receptor Gr64a from Drosophila melanogaster in complex with maltose
Method: EM (single particle) / Resolution: 2.6 Å

38133

8x82

EMDB-38133, PDB-8x82:
The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster
Method: EM (single particle) / Resolution: 2.8 Å

38134

8x83

EMDB-38134, PDB-8x83:
The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster in complex with fructose
Method: EM (single particle) / Resolution: 2.8 Å

38135

8x84

EMDB-38135, PDB-8x84:
The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster in complex with fructose and calcium
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-FRU:
beta-D-fructofuranose / Fructose

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-CA:
Unknown entry

Source
  • drosophila melanogaster (fruit fly)
KeywordsMEMBRANE PROTEIN / gustatory receptor / Gr43a / ligand-gated ion channel / fructose / Gr64a / sucrose / maltose

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