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TitleMultiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway.
Journal, issue, pagesInt J Mol Sci, Vol. 24, Issue 8, Year 2023
Publish dateApr 19, 2023
AuthorsHui-Ju Huang / Sen-Lin Tang / Yuan-Chih Chang / Hao-Ching Wang / Tze Hann Ng / Rees F Garmann / Yu-Wen Chen / Jiun-Yan Huang / Ramya Kumar / Sheng-Hsiung Chang / Shang-Rung Wu / Chih-Yu Chao / Kyoko Matoba / Iwasaki Kenji / William M Gelbart / Tzu-Ping Ko / Hwei-Jiung Andrew Wang / Chu-Fang Lo / Li-Li Chen / Han-Ching Wang /
PubMed AbstractWhite spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable ...White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV.
External linksInt J Mol Sci / PubMed:37108688 / PubMed Central
MethodsEM (single particle)
Resolution9.5 - 10.0 Å
Structure data

EMDB-35598: cryo-EM structure of the middle part of the shrimp white spot syndrome virus nucleocapsid (wide type)
Method: EM (single particle) / Resolution: 10.0 Å

EMDB-35600: Cryo-Em structure of the middle part of the shrimp white spot syndrome virus nucleocapsid (narrow type)
Method: EM (single particle) / Resolution: 9.5 Å

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