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TitleStructural insights into the binding mechanism of Clr4 methyltransferase to H3K9 methylated nucleosome.
Journal, issue, pagesSci Rep, Vol. 14, Issue 1, Page 5438, Year 2024
Publish dateMar 5, 2024
AuthorsChristopher Saab / Joseph Stephan / Elias Akoury /
PubMed AbstractThe establishment and maintenance of heterochromatin, a specific chromatin structure essential for genomic stability and regulation, rely on intricate interactions between chromatin-modifying enzymes ...The establishment and maintenance of heterochromatin, a specific chromatin structure essential for genomic stability and regulation, rely on intricate interactions between chromatin-modifying enzymes and nucleosomal histone proteins. However, the precise trigger for these modifications remains unclear, thus highlighting the need for a deeper understanding of how methyltransferases facilitate histone methylation among others. Here, we investigate the molecular mechanisms underlying heterochromatin assembly by studying the interaction between the H3K9 methyltransferase Clr4 and H3K9-methylated nucleosomes. Using a combination of liquid-state nuclear magnetic resonance spectroscopy and cryo-electron microscopy, we elucidate the structural basis of Clr4 binding to H3K9-methylated nucleosomes. Our results reveal that Clr4 engages with nucleosomes through its chromodomain and disordered regions to promote de novo methylation. This study provides crucial insights into the molecular mechanisms governing heterochromatin formation by highlighting the significance of chromatin-modifying enzymes in genome regulation and disease pathology.
External linksSci Rep / PubMed:38443490 / PubMed Central
MethodsEM (single particle)
Resolution4.5 Å
Structure data

EMDB-35060: Clr4-H3K9 Nucleosome complex
Method: EM (single particle) / Resolution: 4.5 Å

Source
  • Escherichia coli (E. coli)

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