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TitleCms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release.
Journal, issue, pagesCell Rep, Vol. 41, Issue 8, Page 111684, Year 2022
Publish dateNov 22, 2022
AuthorsBenjamin Lau / Olga Beine-Golovchuk / Markus Kornprobst / Jingdong Cheng / Dieter Kressler / Beáta Jády / Tamás Kiss / Roland Beckmann / Ed Hurt /
PubMed AbstractRibosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target ...Ribosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target sites. Here, we report a role for Cms1 in such a process. Initially, we discovered CMS1 as a null suppressor of a nop14 mutant impaired in Rrp12-Enp1 factor recruitment to the 90S. Further investigations detected Cms1 at the 18S rRNA 3' major domain of an early 90S that carried H/ACA snR83, which is known to guide pseudouridylation at two target sites within the same subdomain. Cms1 co-precipitates with many 90S factors, but Rrp12-Enp1 encircling the 3' major domain in the mature 90S is decreased. We suggest that Cms1 associates with the 3' major domain during early 90S biogenesis to restrict premature Rrp12-Enp1 binding but allows snR83 to timely perform its modification role before the next 90S assembly steps coupled with Cms1 release take place.
External linksCell Rep / PubMed:36417864 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 6.7 Å
Structure data

EMDB-34283: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae (Nop14-5Ala), state post-A1
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-34284: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae (Rcl1/Cms1 KO)
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-34285: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae (Nop14 5Ala, Cms1 KO), state B2
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-34286: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae (Nop14 5Ala, Cms1 KO), state b
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • Saccaromyces cerevisiae (brewer's yeast)
  • Saccharomyces cerevisiae (brewer's yeast)

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