Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
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Journal
IF	>30 >20 >10 >5 all

Title	Conformational changes in the human Cx43/GJA1 gap junction channel visualized using cryo-EM.
Journal, issue, pages	Nat Commun, Vol. 14, Issue 1, Page 931, Year 2023
Publish date	Feb 18, 2023
Authors	Hyuk-Joon Lee / Hyung Jin Cha / Hyeongseop Jeong / Seu-Na Lee / Chang-Won Lee / Minsoo Kim / Jejoong Yoo / Jae-Sung Woo /
PubMed Abstract	Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The hemichannels dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). ...Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The hemichannels dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). We report the cryo-electron microscopy structures of Cx43 GJICh, revealing the dynamic equilibrium state of various channel conformations in detergents and lipid nanodiscs. We identify three different N-terminal helix conformations of Cx43-gate-covering (GCN), pore-lining (PLN), and flexible intermediate (FIN)-that are randomly distributed in purified GJICh particles. The conformational equilibrium shifts to GCN by cholesteryl hemisuccinates and to PLN by C-terminal truncations and at varying pH. While GJIChs that mainly comprise GCN protomers are occluded by lipids, those containing conformationally heterogeneous protomers show markedly different pore sizes. We observe an α-to-π-helix transition in the first transmembrane helix, which creates a side opening to the membrane in the FIN and PLN conformations. This study provides basic structural information to understand the mechanisms of action and regulation of Cx43 GJICh.
External links	Nat Commun / PubMed:36805660 / PubMed Central
Methods	EM (single particle)
Resolution	2.3 - 4.0 Å
Structure data	31495 7f92 EMDB-31495, PDB-7f92: Structure of connexin43/Cx43/GJA1 gap junction intercellular channel in LMNG/CHS detergents at pH ~8.0 Method: EM (single particle) / Resolution: 3.1 Å 31496 7f93 EMDB-31496, PDB-7f93: Structure of connexin43/Cx43/GJA1 gap junction intercellular channel in nanodiscs with soybean lipids at pH ~8.0 Method: EM (single particle) / Resolution: 3.6 Å 31497 7f94 EMDB-31497, PDB-7f94: Structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel with two conformationally different hemichannels Method: EM (single particle) / Resolution: 3.6 Å 33391 7xq9 EMDB-33391, PDB-7xq9: Structure of connexin43/Cx43/GJA1 gap junction intercellular channel in GDN detergents at pH ~8.0 Method: EM (single particle) / Resolution: 3.3 Å 33392 7xqb EMDB-33392, PDB-7xqb: Structure of connexin43/Cx43/GJA1 gap junction intercellular channel in POPE/CHS nanodiscs at pH ~8.0 Method: EM (single particle) / Resolution: 3.0 Å 33393 7xqd EMDB-33393, PDB-7xqd: Structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE/CHS nanodiscs (C1 symmetry) Method: EM (single particle) / Resolution: 2.7 Å 33394 7xqf EMDB-33394, PDB-7xqf: Structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE/CHS nanodiscs Method: EM (single particle) / Resolution: 2.3 Å 33395 7xqg EMDB-33395, PDB-7xqg: Hemichannel-focused structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE nanodiscs (GCN conformation) Method: EM (single particle) / Resolution: 3.8 Å 33396 7xqh EMDB-33396, PDB-7xqh: Hemichannel-focused structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE nanodiscs (GCN-TM1i conformation) Method: EM (single particle) / Resolution: 3.8 Å 33397 7xqi EMDB-33397, PDB-7xqi: Hemichannel-focused structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE nanodiscs (FIN conformation) Method: EM (single particle) / Resolution: 3.7 Å 33398 7xqj EMDB-33398, PDB-7xqj: Hemichannel-focused structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE nanodiscs (PLN conformation) Method: EM (single particle) / Resolution: 4.0 Å EMDB-33399: Consensus map of connexin43/Cx43/GJA1 gap junction intercellular channel in LMNG/CHS detergents at pH ~6.9 Method: EM (single particle) / Resolution: 3.3 Å
Chemicals	ChemComp-C14: TETRADECANE / Tetradecane ChemComp-HOH: WATER / Water ChemComp-Y01: CHOLESTEROL HEMISUCCINATE ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / phospholipid*YM / Phosphatidylethanolamine
Source	homo sapiens (human)
Keywords	MEMBRANE PROTEIN / Cx43 / Connexin / Gap junction channel / Gating mechanism