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TitleMembrane translocation process revealed by in situ structures of type II secretion system secretins.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 4025, Year 2023
Publish dateJul 7, 2023
AuthorsZhili Yu / Yaoming Wu / Muyuan Chen / Tong Huo / Wei Zheng / Steven J Ludtke / Xiaodong Shi / Zhao Wang /
PubMed AbstractThe GspD secretin is the outer membrane channel of the bacterial type II secretion system (T2SS) which secrets diverse toxins that cause severe diseases such as diarrhea and cholera. GspD needs to ...The GspD secretin is the outer membrane channel of the bacterial type II secretion system (T2SS) which secrets diverse toxins that cause severe diseases such as diarrhea and cholera. GspD needs to translocate from the inner to the outer membrane to exert its function, and this process is an essential step for T2SS to assemble. Here, we investigate two types of secretins discovered so far in Escherichia coli, GspD, and GspD. By electron cryotomography subtomogram averaging, we determine in situ structures of key intermediate states of GspD and GspD in the translocation process, with resolution ranging from 9 Å to 19 Å. In our results, GspD and GspD present entirely different membrane interaction patterns and ways of transitioning the peptidoglycan layer. From this, we hypothesize two distinct models for the membrane translocation of GspD and GspD, providing a comprehensive perspective on the inner to outer membrane biogenesis of T2SS secretins.
External linksNat Commun / PubMed:37419909 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution9.0 - 19.0 Å
Structure data

EMDB-29696: In situ structure of GspD-beta-GspS complex on the bacterial outer membrane
Method: EM (subtomogram averaging) / Resolution: 19.0 Å

EMDB-29697: In situ structure of GspD-beta-GspS complex on the bacterial outer membrane when GspD-beta is overexpressed solely
Method: EM (subtomogram averaging) / Resolution: 16.0 Å

EMDB-29698: In situ structure of GspD-beta on the bacterial inner membrane
Method: EM (subtomogram averaging) / Resolution: 14.0 Å

EMDB-29702: In situ structure of GspD-alpha on the bacterial inner membrane
Method: EM (subtomogram averaging) / Resolution: 9.0 Å

EMDB-29703: In situ structure of GspD-alpha on the bacterial outer membrane
Method: EM (subtomogram averaging) / Resolution: 16.0 Å

Source
  • Escherichia coli ETEC H10407 (bacteria)
  • Escherichia coli K-12 (bacteria)

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