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TitleComprehensive structure and functional adaptations of the yeast nuclear pore complex.
Journal, issue, pagesCell, Vol. 185, Issue 2, Page 361-378.e25, Year 2022
Publish dateJan 20, 2022
AuthorsChristopher W Akey / Digvijay Singh / Christna Ouch / Ignacia Echeverria / Ilona Nudelman / Joseph M Varberg / Zulin Yu / Fei Fang / Yi Shi / Junjie Wang / Daniel Salzberg / Kangkang Song / Chen Xu / James C Gumbart / Sergey Suslov / Jay Unruh / Sue L Jaspersen / Brian T Chait / Andrej Sali / Javier Fernandez-Martinez / Steven J Ludtke / Elizabeth Villa / Michael P Rout /
PubMed AbstractNuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub- ...Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional layers. These connectors may be targets for phosphorylation and regulated disassembly in cells with an open mitosis. Moreover, some nucleoporin pairs and transport factors have similar interaction motifs, which suggests an evolutionary and mechanistic link between assembly and transport. We provide evidence for three major NPC variants that may foreshadow functional specializations at the nuclear periphery. Cryo-electron tomography extended these studies, providing a model of the in situ NPC with a radially expanded inner ring. Our comprehensive model reveals features of the nuclear basket and central transporter, suggests a role for the lumenal Pom152 ring in restricting dilation, and highlights structural plasticity that may be required for transport.
External linksCell / PubMed:34982960 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution7.6 - 37.0 Å
Structure data

EMDB-24224:
Combined 3D structure of the isolated yeast NPC
Method: EM (single particle) / Resolution: 7.6 Å

EMDB-24225:
Low resolution, multi-part 3D structure of the isolated yeast NPC
Method: EM (single particle) / Resolution: 24.0 Å

24231

7n84

EMDB-24231, PDB-7n84:
Double nuclear outer ring from the isolated yeast NPC
Method: EM (single particle) / Resolution: 11.6 Å

24232

7n85

EMDB-24232, PDB-7n85:
Inner ring spoke from the isolated yeast NPC
Method: EM (single particle) / Resolution: 7.6 Å

24258

7n9f

EMDB-24258, PDB-7n9f:
Structure of the in situ yeast NPC
Method: EM (subtomogram averaging) / Resolution: 37.0 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • Baker's yeast (brewer's yeast)
KeywordsTRANSLOCASE / nuclear pore complex / outer ring / Nup84 complex / inner ring / spoke / NPC / nucleocytoplasmic transport

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