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TitleNative architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry.
Journal, issue, pagesCurr Biol, Vol. 23, Issue 17, Page 1620-1628, Year 2013
Publish dateSep 9, 2013
AuthorsPaul Guichard / Virginie Hachet / Norbert Majubu / Aitana Neves / Davide Demurtas / Natacha Olieric / Isabelle Fluckiger / Akinori Yamada / Kumiko Kihara / Yuichiro Nishida / Shigeharu Moriya / Michel O Steinmetz / Yuichi Hongoh / Pierre Gönczy /
PubMed AbstractBACKGROUND: Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel ...BACKGROUND: Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel that dictates the 9-fold symmetry of centrioles. Although the cartwheel architecture has been recently analyzed, how it connects to the peripheral microtubules is not understood. More generally, a high-resolution view of the proximal region of the centriole is lacking, thus limiting understanding of the underlying assembly mechanisms.
RESULTS: We report the complete architecture of the Trichonympha centriole proximal region using cryotomography. The resulting 3D map reveals several features, including additional densities in the cartwheel that exhibit a 9-fold symmetrical arrangement, as well as the structure of the Pinhead and the A-C linker that connect to microtubules. Moreover, we uncover striking chiral features that might impart directionality to the entire centriole. Furthermore, we identify Trichonympha SAS-6 and demonstrate that it localizes to the cartwheel in vivo.
CONCLUSIONS: Our work provides unprecedented insight into the architecture of the centriole proximal region, which is key for a thorough understanding of the mechanisms governing centriole assembly.
External linksCurr Biol / PubMed:23932403
MethodsEM (subtomogram averaging)
Resolution38.0 - 42.0 Å
Structure data

EMDB-11049:
Electron cryo-tomography reconstruction and subvolume averaging of the proximal basal body triplet from Trichonympha
Method: EM (subtomogram averaging) / Resolution: 42.0 Å

EMDB-2329:
electron cryo-tomography reconstruction and subvolume averaging of the Trichonympha proximal basal body
Method: EM (subtomogram averaging) / Resolution: 38.0 Å

EMDB-2330:
Electron cryo-tomography reconstruction and subvolume averaging of the proximal basal body triplet from Trichonympha
Method: EM (subtomogram averaging) / Resolution: 42.0 Å

Source
  • Trichonympha sp. (eukaryote)
  • Trichonympha (eukaryote)

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