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TitleThe In Situ Structure of Parkinson's Disease-Linked LRRK2.
Journal, issue, pagesCell, Vol. 182, Issue 6, Page 1508-1518.e16, Year 2020
Publish dateSep 17, 2020
AuthorsReika Watanabe / Robert Buschauer / Jan Böhning / Martina Audagnotto / Keren Lasker / Tsan-Wen Lu / Daniela Boassa / Susan Taylor / Elizabeth Villa /
PubMed AbstractMutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent cause of familial Parkinson's disease. LRRK2 is a multi-domain protein containing a kinase and GTPase. Using correlative light ...Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent cause of familial Parkinson's disease. LRRK2 is a multi-domain protein containing a kinase and GTPase. Using correlative light and electron microscopy, in situ cryo-electron tomography, and subtomogram analysis, we reveal a 14-Å structure of LRRK2 bearing a pathogenic mutation that oligomerizes as a right-handed double helix around microtubules, which are left-handed. Using integrative modeling, we determine the architecture of LRRK2, showing that the GTPase and kinase are in close proximity, with the GTPase closer to the microtubule surface, whereas the kinase is exposed to the cytoplasm. We identify two oligomerization interfaces mediated by non-catalytic domains. Mutation of one of these abolishes LRRK2 microtubule-association. Our work demonstrates the power of cryo-electron tomography to generate models of previously unsolved structures in their cellular environment.
External linksCell / PubMed:32783917 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution14.0 - 18.0 Å
Structure data

20825

6xr4

EMDB-20825: In situ structure of LRRK2(I2020T)-Microtubule: Microtubule_bound_LRRK2(I2020T)_Tight Mask_A
PDB-6xr4: Integrative in situ structure of Parkinsons disease-linked human LRRK2
Method: EM (subtomogram averaging) / Resolution: 14.0 Å

EMDB-20826:
In situ structure of Parkinson's disease-linked Microtubule-Bound human LRRK2(I2020T) MASK B
Method: EM (subtomogram averaging) / Resolution: 18.0 Å

EMDB-20827:
Cryo-electron tomogram of FIB-milled HEK293 cells expressing pathogenic LRRK2(I2020T)mutant
Method: EM (tomography)

EMDB-20828:
Cryo-electron tomogram of FIB-milled HEK293 cells treated with Taxol
Method: EM (tomography)

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / TRANSFERASE / kinase / GTPase / Parkinson's Disease / pseudo-kinase

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