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Title2.7 Å cryo-EM structure of rotavirus core protein VP3, a unique capping machine with a helicase activity.
Journal, issue, pagesSci Adv, Vol. 6, Issue 16, Page eaay6410, Year 2020
Publish dateApr 15, 2020
AuthorsDilip Kumar / Xinzhe Yu / Sue E Crawford / Rodolfo Moreno / Joanita Jakana / Banumathi Sankaran / Ramakrishnan Anish / Soni Kaundal / Liya Hu / Mary K Estes / Zhao Wang / B V Venkataram Prasad /
PubMed AbstractIn many viruses, including rotavirus (RV), the major pathogen of infantile gastroenteritis, capping of viral messenger RNAs is a pivotal step for efficient translation of the viral genome. In RV, VP3 ...In many viruses, including rotavirus (RV), the major pathogen of infantile gastroenteritis, capping of viral messenger RNAs is a pivotal step for efficient translation of the viral genome. In RV, VP3 caps the nascent transcripts synthesized from the genomic dsRNA segments by the RV polymerase VP1 within the particle core. Here, from cryo-electron microscopy, x-ray crystallography, and biochemical analyses, we show that VP3 forms a stable tetrameric assembly with each subunit having a modular domain organization, which uniquely integrates five distinct enzymatic steps required for capping the transcripts. In addition to the previously known guanylyl- and methyltransferase activities, we show that VP3 exhibits hitherto unsuspected RNA triphosphatase activity necessary for initiating transcript capping and RNA helicase activity likely required for separating the RNA duplex formed transiently during endogenous transcription. From our studies, we propose a new mechanism for how VP3 inside the virion core caps the nascent transcripts exiting from the polymerase.
External linksSci Adv / PubMed:32494598 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.5 - 11.8 Å
Structure data

EMDB-20159:
Rotavirus A-VP3-8mer ssRNA complex (RVA-VP3-RNA)
Method: EM (single particle) / Resolution: 11.8 Å

PDB-6o3v:
Crystal structure for RVA-VP3
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

Chemicals

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE / Guanosine monophosphate

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

Source
  • rotavirus a
KeywordsSTRUCTURAL PROTEIN / Rotavirus / VP3 / Capping enzyme / Guanylyl transferase / Methyl transferase / RTPase / PDE

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