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TitleStepwise assembly and release of Tc toxins from Yersinia entomophaga.
Journal, issue, pagesNat Microbiol, Vol. 9, Issue 2, Page 405-420, Year 2024
Publish dateFeb 5, 2024
AuthorsMiki Feldmüller / Charles F Ericson / Pavel Afanasyev / Yun-Wei Lien / Gregor L Weiss / Florian Wollweber / Marion Schoof / Mark Hurst / Martin Pilhofer /
PubMed AbstractTc toxins are virulence factors of bacterial pathogens. Although their structure and intoxication mechanism are well understood, it remains elusive where this large macromolecular complex is ...Tc toxins are virulence factors of bacterial pathogens. Although their structure and intoxication mechanism are well understood, it remains elusive where this large macromolecular complex is assembled and how it is released. Here we show by an integrative multiscale imaging approach that Yersinia entomophaga Tc (YenTc) toxin components are expressed only in a subpopulation of cells that are 'primed' with several other potential virulence factors, including filaments of the protease M66/StcE. A phage-like lysis cassette is required for YenTc release; however, before resulting in complete cell lysis, the lysis cassette generates intermediate 'ghost' cells, which may serve as assembly compartments and become packed with assembled YenTc holotoxins. We hypothesize that this stepwise mechanism evolved to minimize the number of cells that need to be killed. The occurrence of similar lysis cassettes in diverse organisms indicates a conserved mechanism for Tc toxin release that may apply to other extracellular macromolecular machines.
External linksNat Microbiol / PubMed:38316932 / PubMed Central
MethodsEM (tomography) / EM (subtomogram averaging)
Resolution9.89 - 31.1 Å
Structure data

EMDB-18953: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells
Method: EM (tomography)

EMDB-18954: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells
Method: EM (tomography)

EMDB-18955: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells
Method: EM (tomography)

EMDB-18957: Cryo-electron tomogram of Yersinia entomophaga MH96 cells
Method: EM (tomography)

EMDB-18958: Cryo-electron tomogram of Yersinia entomophaga MH96 cells
Method: EM (tomography)

EMDB-18960: Cryo-electron tomogram of Yersinia entomophaga delta LC cells
Method: EM (tomography)

EMDB-18961: Cryo-electron tomogram of mechanically cryo-milled Yersinia entomophaga delta LC cells
Method: EM (tomography)

EMDB-18962: Cryo-electron tomogram of a lysate preparation of Yersinia entomophaga delta LC cells
Method: EM (tomography)

EMDB-18970: Subtomogram average of M66 filaments in Yersinia entomophaga cells
Method: EM (subtomogram averaging) / Resolution: 9.89 Å

EMDB-18971: Subtomogram average of YenTc-Chi2-sfGFP from Yersinia entomophaga chi2-sfGFP
Method: EM (subtomogram averaging) / Resolution: 29.6 Å

EMDB-18972: Subtomogram average of YenTc from Yersinia entomophaga MH96
Method: EM (subtomogram averaging) / Resolution: 31.1 Å

EMDB-19370: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells
Method: EM (tomography)

EMDB-19371: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells
Method: EM (tomography)

EMDB-19372: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells
Method: EM (tomography)

EMDB-19373: Cryo-electron tomogram of Yersinia entomophaga delta YenTc cells
Method: EM (tomography)

EMDB-19374: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells
Method: EM (tomography)

EMDB-19375: Cryo-electron tomogram of Yersinia entomophaga chi2-sfGFP cells
Method: EM (tomography)

EMDB-19376: Cryo-electron tomogram of Yersinia entomophaga delta LC delta YenTc cells
Method: EM (tomography)

EMDB-19377: Cryo-electron tomogram of Yersinia entomophaga MH96 cells grown at 37 degrees
Method: EM (tomography)

EMDB-19378: Cryo-electron tomogram of Yersinia entomophaga delta LC cells grown at 37 degrees
Method: EM (tomography)

EMDB-19379: Cryo-electron tomogram of Yersinia entomophaga delta LC delta M66 cells
Method: EM (tomography)

EMDB-19380: Cryo-electron tomogram of Yersinia entomophaga delta LC delta M66 cells
Method: EM (tomography)

EMDB-19381: Cryo-electron tomogram of a lysate preparation of Yersinia entomophaga delta LC delta M66 cells
Method: EM (tomography)

Source
  • Yersinia entomophaga (bacteria)

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