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TitleStructural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules.
Journal, issue, pagesScience, Vol. 382, Issue 6675, Page 1184-1190, Year 2023
Publish dateDec 8, 2023
AuthorsKyle W Muir / Christopher Batters / Tom Dendooven / Jing Yang / Ziguo Zhang / Alister Burt / David Barford /
PubMed AbstractKinetochores couple chromosomes to the mitotic spindle to segregate the genome during cell division. An error correction mechanism drives the turnover of kinetochore-microtubule attachments until ...Kinetochores couple chromosomes to the mitotic spindle to segregate the genome during cell division. An error correction mechanism drives the turnover of kinetochore-microtubule attachments until biorientation is achieved. The structural basis for how kinetochore-mediated chromosome segregation is accomplished and regulated remains an outstanding question. In this work, we describe the cryo-electron microscopy structure of the budding yeast outer kinetochore Ndc80 and Dam1 ring complexes assembled onto microtubules. Complex assembly occurs through multiple interfaces, and a staple within Dam1 aids ring assembly. Perturbation of key interfaces suppresses yeast viability. Force-rupture assays indicated that this is a consequence of impaired kinetochore-microtubule attachment. The presence of error correction phosphorylation sites at Ndc80-Dam1 ring complex interfaces and the Dam1 staple explains how kinetochore-microtubule attachments are destabilized and reset.
External linksScience / PubMed:38060647 / PubMed Central
MethodsEM (single particle)
Resolution3.15 - 4.11 Å
Structure data

18246

8q84

EMDB-18246, PDB-8q84:
Outer kinetochore Dam1 protomer dimer Ndc80-Nuf2 coiled-coil complex
Method: EM (single particle) / Resolution: 3.15 Å

18247

8q85

EMDB-18247, PDB-8q85:
Outer kinetochore Dam1 protomer monomer Ndc80-Nuf2 coiled-coil complex
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-18485: Ndc80c microtubule complex
Method: EM (single particle) / Resolution: 4.11 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsCELL CYCLE / Kinetochore / microtubule / error correction / chromosome segregation

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