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TitleCrystal structure of bacteriophage SPP1 distal tail protein (gp19.1): a baseplate hub paradigm in gram-positive infecting phages.
Journal, issue, pagesJ Biol Chem, Vol. 285, Issue 47, Page 36666-36673, Year 2010
Publish dateNov 19, 2010
AuthorsDavid Veesler / Gautier Robin / Julie Lichière / Isabelle Auzat / Paulo Tavares / Patrick Bron / Valérie Campanacci / Christian Cambillau /
PubMed AbstractSiphophage SPP1 infects the gram-positive bacterium Bacillus subtilis using its long non-contractile tail and tail-tip. Electron microscopy (EM) previously allowed a low resolution assignment of most ...Siphophage SPP1 infects the gram-positive bacterium Bacillus subtilis using its long non-contractile tail and tail-tip. Electron microscopy (EM) previously allowed a low resolution assignment of most orf products belonging to these regions. We report here the structure of the SPP1 distal tail protein (Dit, gp19.1). The combination of x-ray crystallography, EM, and light scattering established that Dit is a back-to-back dimer of hexamers. However, Dit fitting in the virion EM maps was only possible with a hexamer located between the tail-tube and the tail-tip. Structure comparison revealed high similarity between Dit and a central component of lactophage baseplates. Sequence similarity search expanded its relatedness to several phage proteins, suggesting that Dit is a docking platform for the tail adsorption apparatus in Siphoviridae infecting gram-positive bacteria and that its architecture is a paradigm for these hub proteins. Dit structural similarity extends also to non-contractile and contractile phage tail proteins (gpV(N) and XkdM) as well as to components of the bacterial type 6 secretion system, supporting an evolutionary connection between all these devices.
External linksJ Biol Chem / PubMed:20843802 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.95 - 21.5 Å
Structure data

EMDB-1779:
EM structure of bacteriophage SPP1 distal tail protein (GP 19.1): a baseplate hub paradigm in gram positive infecting phages
Method: EM (single particle) / Resolution: 21.5 Å

PDB-2x8k:
Crystal Structure of SPP1 Dit (gp 19.1) Protein, a Paradigm of Hub Adsorption Apparatus in Gram-positive Infecting Phages.
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

Source
  • bacillus phage spp1 (virus)
KeywordsVIRAL PROTEIN / DISTAL TAIL PROTEIN

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