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TitleCryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state.
Journal, issue, pagesNat Microbiol, Vol. 8, Issue 10, Page 1834-1845, Year 2023
Publish dateSep 14, 2023
AuthorsMathew McLaren / Rebecca Conners / Michail N Isupov / Patricia Gil-Díez / Lavinia Gambelli / Vicki A M Gold / Andreas Walter / Sean R Connell / Bryony Williams / Bertram Daum /
PubMed AbstractTranslational control is an essential process for the cell to adapt to varying physiological or environmental conditions. To survive adverse conditions such as low nutrient levels, translation can be ...Translational control is an essential process for the cell to adapt to varying physiological or environmental conditions. To survive adverse conditions such as low nutrient levels, translation can be shut down almost entirely by inhibiting ribosomal function. Here we investigated eukaryotic hibernating ribosomes from the microsporidian parasite Spraguea lophii in situ by a combination of electron cryo-tomography and single-particle electron cryo-microscopy. We show that microsporidian spores contain hibernating ribosomes that are locked in a dimeric (100S) state, which is formed by a unique dimerization mechanism involving the beak region. The ribosomes within the dimer are fully assembled, suggesting that they are ready to be activated once the host cell is invaded. This study provides structural evidence for dimerization acting as a mechanism for ribosomal hibernation in microsporidia, and therefore demonstrates that eukaryotes utilize this mechanism in translational control.
External linksNat Microbiol / PubMed:37709902 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution10.8 - 14.3 Å
Structure data

17448

8p5d

EMDB-17448, PDB-8p5d:
Spraguea lophii ribosome in the closed conformation by cryo sub tomogram averaging
Method: EM (subtomogram averaging) / Resolution: 10.8 Å

17457

8p60

EMDB-17457, PDB-8p60:
Spraguea lophii ribosome dimer
Method: EM (subtomogram averaging) / Resolution: 14.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • spraguea lophii 42_110 (fungus)
KeywordsRIBOSOME / Microsporidia

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