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TitleStructural insights into human TFIIIC promoter recognition.
Journal, issue, pagesSci Adv, Vol. 9, Issue 27, Page eadh2019, Year 2023
Publish dateJul 7, 2023
AuthorsWolfram Seifert-Davila / Mathias Girbig / Luis Hauptmann / Thomas Hoffmann / Sebastian Eustermann / Christoph W Müller /
PubMed AbstractTranscription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB ...Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The τB module recognizes the B-box via DNA shape and sequence readout through the assembly of multiple winged-helix domains. TFIIIC220 forms an integral part of both τA and τB connecting the two subcomplexes via a ~550-amino acid residue flexible linker. Our data provide a structural mechanism by which high-affinity B-box recognition anchors TFIIIC to promoter DNA and permits scanning for low-affinity A-boxes and TFIIIB for Pol III activation.
External linksSci Adv / PubMed:37418517
MethodsEM (single particle)
Resolution3.2 - 3.8 Å
Structure data

16713

8cli

EMDB-16713: Local refinement map of TFIIIC TauB-DNA monomer
PDB-8cli: TFIIIC TauB-DNA monomer
Method: EM (single particle) / Resolution: 3.2 Å

16714

8clj

EMDB-16714, PDB-8clj:
TFIIIC TauB-DNA dimer
Method: EM (single particle) / Resolution: 3.2 Å

16715

8clk

EMDB-16715: TFIIIC TauA complex map
PDB-8clk: TFIIIC TauA complex
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-16716: TFIIIC TauA complex map (sample without DNA)
Method: EM (single particle) / Resolution: 3.8 Å

16717

8cll

EMDB-16717, PDB-8cll:
Structural insights into human TFIIIC promoter recognition
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-17446: Consensus map of TauB-DNA dimer (Nu-refinement)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-17447: Local refinement map of TFIIIC TauB-DNA monomer 2
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsTRANSCRIPTION / TFIIIC / tRNA gene / B-Box promoter / DNA recognition

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