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TitleBinding to nucleosome poises human SIRT6 for histone H3 deacetylation.
Journal, issue, pagesElife, Vol. 12, Year 2024
Publish dateFeb 28, 2024
AuthorsEkaterina Smirnova / Emmanuelle Bignon / Patrick Schultz / Gabor Papai / Adam Ben Shem /
PubMed AbstractSirtuin 6 (SIRT6) is an NAD-dependent histone H3 deacetylase that is prominently found associated with chromatin, attenuates transcriptionally active promoters and regulates DNA repair, metabolic ...Sirtuin 6 (SIRT6) is an NAD-dependent histone H3 deacetylase that is prominently found associated with chromatin, attenuates transcriptionally active promoters and regulates DNA repair, metabolic homeostasis and lifespan. Unlike other sirtuins, it has low affinity to free histone tails but demonstrates strong binding to nucleosomes. It is poorly understood how SIRT6 docking on nucleosomes stimulates its histone deacetylation activity. Here, we present the structure of human SIRT6 bound to a nucleosome determined by cryogenic electron microscopy. The zinc finger domain of SIRT6 associates tightly with the acidic patch of the nucleosome through multiple arginine anchors. The Rossmann fold domain binds to the terminus of the looser DNA half of the nucleosome, detaching two turns of the DNA from the histone octamer and placing the NAD binding pocket close to the DNA exit site. This domain shows flexibility with respect to the fixed zinc finger and moves with, but also relative to, the unwrapped DNA terminus. We apply molecular dynamics simulations of the histone tails in the nucleosome to show that in this mode of interaction, the active site of SIRT6 is perfectly poised to catalyze deacetylation of the H3 histone tail and that the partial unwrapping of the DNA allows even lysines close to the H3 core to reach the enzyme.
External linksElife / PubMed:38415718 / PubMed Central
MethodsEM (single particle)
Resolution2.94 - 3.79 Å
Structure data

EMDB-16842: SIRT6 bound nucleosome
Method: EM (single particle) / Resolution: 2.94 Å

EMDB-16843: Nucleosome bound human SIRT6
Method: EM (single particle) / Resolution: 3.79 Å

16845

8of4

EMDB-16845: Nucleosome Bound human SIRT6 (composite structure)
PDB-8of4: Nucleosome Bound human SIRT6 (Composite)
Method: EM (single particle) / Resolution: 2.94 Å

EMDB-16861: Sirt6 bound nucleosome - consensus map
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-18497: Human Sirtuin 6 in complex with nucleosome - structure showing H2A tail path
Method: EM (single particle) / Resolution: 3.78 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • xenopus laevis (African clawed frog)
  • synthetic construct (others)
  • Escherichia coli (E. coli)
KeywordsGENE REGULATION / Transferase / Deacetylase / Histone H3 deacetylation

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