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TitleBiophysical basis of filamentous phage tactoid-mediated antibiotic tolerance in P. aeruginosa.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 8429, Year 2023
Publish dateDec 19, 2023
AuthorsJan Böhning / Miles Graham / Suzanne C Letham / Luke K Davis / Ulrike Schulze / Phillip J Stansfeld / Robin A Corey / Philip Pearce / Abul K Tarafder / Tanmay A M Bharat /
PubMed AbstractInoviruses are filamentous phages infecting numerous prokaryotic phyla. Inoviruses can self-assemble into mesoscale structures with liquid-crystalline order, termed tactoids, which protect bacterial ...Inoviruses are filamentous phages infecting numerous prokaryotic phyla. Inoviruses can self-assemble into mesoscale structures with liquid-crystalline order, termed tactoids, which protect bacterial cells in Pseudomonas aeruginosa biofilms from antibiotics. Here, we investigate the structural, biophysical, and protective properties of tactoids formed by the P. aeruginosa phage Pf4 and Escherichia coli phage fd. A cryo-EM structure of the capsid from fd revealed distinct biochemical properties compared to Pf4. Fd and Pf4 formed tactoids with different morphologies that arise from differing phage geometries and packing densities, which in turn gave rise to different tactoid emergent properties. Finally, we showed that tactoids formed by either phage protect rod-shaped bacteria from antibiotic treatment, and that direct association with a tactoid is required for protection, demonstrating the formation of a diffusion barrier by the tactoid. This study provides insights into how filamentous molecules protect bacteria from extraneous substances in biofilms and in host-associated infections.
External linksNat Commun / PubMed:38114502 / PubMed Central
MethodsEM (helical sym.)
Resolution3.2 Å
Structure data

16657

8ch5

EMDB-16657, PDB-8ch5:
Cryo-EM structure of the fd bacteriophage capsid major coat protein pVIII
Method: EM (helical sym.) / Resolution: 3.2 Å

Source
  • escherichia coli (E. coli)
KeywordsVIRUS / Bacteriophage / fd / inovirus / ff / helical / phage / filamentous

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