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TitleCryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 2724, Year 2023
Publish dateMay 11, 2023
AuthorsRebecca Conners / Rayén Ignacia León-Quezada / Mathew McLaren / Nicholas J Bennett / Bertram Daum / Jasna Rakonjac / Vicki A M Gold /
PubMed AbstractPhages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular ...Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular biology and biotechnology. This is particularly true for the Ff (f1, fd or M13) phages, which represent a widely distributed group of filamentous viruses. Over nearly five decades, Ffs have seen an extraordinary range of applications, yet the complete structure of the phage capsid and consequently the mechanisms of infection and assembly remain largely mysterious. In this work, we use cryo-electron microscopy and a highly efficient system for production of short Ff-derived nanorods to determine a structure of a filamentous virus including the tips. We show that structure combined with mutagenesis can identify phage domains that are important in bacterial attack and for release of new progeny, allowing new models to be proposed for the phage lifecycle.
External linksNat Commun / PubMed:37169795 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution2.58 - 2.97 Å
Structure data

15831

8b3o

EMDB-15831, PDB-8b3o:
CryoEM structure of the pointy tip (proteins pIII/pVI/pVIII) from the f1 filamentous bacteriophage
Method: EM (single particle) / Resolution: 2.97 Å

15832

8b3p

EMDB-15832, PDB-8b3p:
CryoEM structure of the round tip (proteins pVII/pVIII/pIX) from the f1 filamentous bacteriophage
Method: EM (single particle) / Resolution: 2.81 Å

15833

8b3q

EMDB-15833, PDB-8b3q:
CryoEM structure of the central filamentous region of the f1 filamentous bacteriophage, consisting of the major capsid protein pVIII
Method: EM (helical sym.) / Resolution: 2.58 Å

Source
  • enterobacteria phage f1 (virus)
KeywordsVIRUS / Viral proteins / Infection / assembly / capsid

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