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Structure paper

TitleThe giant mimivirus 1.2 Mb genome is elegantly organized into a 30-nm diameter helical protein shield.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateJul 28, 2022
AuthorsAlejandro Villalta / Alain Schmitt / Leandro F Estrozi / Emmanuelle R J Quemin / Jean-Marie Alempic / Audrey Lartigue / Vojtěch Pražák / Lucid Belmudes / Daven Vasishtan / Agathe M G Colmant / Flora A Honoré / Yohann Couté / Kay Grünewald / Chantal Abergel /
PubMed AbstractMimivirus is the prototype of the family of giant dsDNA viruses. Little is known about the organization of the 1.2 Mb genome inside the membrane-limited nucleoid filling the ~0.5 µm icosahedral ...Mimivirus is the prototype of the family of giant dsDNA viruses. Little is known about the organization of the 1.2 Mb genome inside the membrane-limited nucleoid filling the ~0.5 µm icosahedral capsids. Cryo-electron microscopy, cryo-electron tomography, and proteomics revealed that it is encased into a ~30-nm diameter helical protein shell surprisingly composed of two GMC-type oxidoreductases, which also form the glycosylated fibrils decorating the capsid. The genome is arranged in 5- or 6-start left-handed super-helices, with each DNA-strand lining the central channel. This luminal channel of the nucleoprotein fiber is wide enough to accommodate oxidative stress proteins and RNA polymerase subunits identified by proteomics. Such elegant supramolecular organization would represent a remarkable evolutionary strategy for packaging and protecting the genome, in a state ready for immediate transcription upon unwinding in the host cytoplasm. The parsimonious use of the same protein in two unrelated substructures of the virion is unexpected for a giant virus with thousand genes at its disposal.
External linksElife / PubMed:35900198 / PubMed Central
MethodsEM (single particle) / EM (helical sym.) / EM (tomography)
Resolution3.3 - 4.0 Å
Structure data

13641

7ptv

EMDB-13641, PDB-7ptv:
Structure of the Mimivirus genomic fibre asymmetric unit
Method: EM (single particle) / Resolution: 3.3 Å

14353

7yx3

EMDB-14353, PDB-7yx3:
Structure of the Mimivirus genomic fibre in its compact 6-start helix form
Method: EM (helical sym.) / Resolution: 4.0 Å

14354

7yx4

EMDB-14354, PDB-7yx4:
Structure of the Mimivirus genomic fibre in its compact 5-start helix form
Method: EM (helical sym.) / Resolution: 3.7 Å

14355

7yx5

EMDB-14355, PDB-7yx5:
Structure of the Mimivirus genomic fibre in its relaxed 5-start helix form
Method: EM (helical sym.) / Resolution: 3.7 Å

EMDB-15627: Tomogram of the Mimivirus genomic fiber
Method: EM (tomography)

EMDB-15628: Tomogram of the Mimivirus genomic fiber
Method: EM (tomography)

EMDB-15629: Tomogram of the Mimivirus genomic fiber
Method: EM (tomography)

EMDB-15630: Tomogram of the Mimivirus genomic fiber
Method: EM (tomography)

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM / Flavin adenine dinucleotide

Source
  • acanthamoeba polyphaga mimivirus
  • Mimivirus
KeywordsVIRAL PROTEIN / Mimivirus / Genomic fibre / Cytoplasmic infectious cycle / 1.2 Mb dsDNA / VIRUS

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