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TitleStructure of a backtracked hexasomal intermediate of nucleosome transcription.
Journal, issue, pagesMol Cell, Vol. 82, Issue 17, Page 3126-33134.e7, Year 2022
Publish dateSep 1, 2022
AuthorsLucas Farnung / Moritz Ochmann / Gaurika Garg / Seychelle M Vos / Patrick Cramer /
PubMed AbstractDuring gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage when the ...During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage when the human elongation factors DSIF, PAF1 complex (PAF), RTF1, SPT6, and TFIIS are present. The cryo-EM structure of an intermediate of the nucleosome passage shows a partially unraveled hexasome that lacks the proximal H2A-H2B dimer and interacts with the RNA Pol II jaw, DSIF, and the CTR9trestle helix. RNA Pol II adopts a backtracked state with the RNA 3' end dislodged from the active site and bound in the RNA Pol II pore. Additional structures and biochemical data show that human TFIIS enters the RNA Pol II pore and stimulates the cleavage of the backtracked RNA and nucleosome passage.
External linksMol Cell / PubMed:35858621
MethodsEM (single particle)
Resolution3.0 Å
Structure data

15129

8a40

EMDB-15129, PDB-8a40:
Structure of mammalian Pol II-TFIIS elongation complex
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • sus scrofa (pig)
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsTRANSCRIPTION / chromatin / rna polymerase II / nucleosome / TFIIS / elongation

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