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TitleMechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella.
Journal, issue, pagesNat Struct Mol Biol, Vol. 29, Issue 12, Page 1170-1177, Year 2022
Publish dateDec 1, 2022
AuthorsJaroslaw Sedzicki / Dongchun Ni / Frank Lehmann / Na Wu / Renato Zenobi / Seunho Jung / Henning Stahlberg / Christoph Dehio /
PubMed AbstractPolysaccharides play critical roles in bacteria, including the formation of protective capsules and biofilms and establishing specific host cell interactions. Their transport across membranes is ...Polysaccharides play critical roles in bacteria, including the formation of protective capsules and biofilms and establishing specific host cell interactions. Their transport across membranes is often mediated by ATP-binding cassette (ABC) transporters, which utilize ATP to translocate diverse molecules. Cyclic β-glucans (CβGs) are critical for host interaction of the Rhizobiales, including the zoonotic pathogen Brucella. CβGs are exported into the periplasmic space by the cyclic glucan transporter (Cgt). The interaction of an ABC transporter with a polysaccharide substrate has not been visualized so far. Here we use single-particle cryoelectron microscopy to elucidate the structures of Cgt from Brucella abortus in four conformational states. The substrate-bound structure reveals an unusual binding pocket at the height of the cytoplasmic leaflet, whereas ADP-vanadate models hint at an alternative mechanism of substrate release. Our work provides insights into the translocation of large, heterogeneous substrates and sheds light on protein-polysaccharide interactions in general.
External linksNat Struct Mol Biol / PubMed:36456825
MethodsEM (single particle)
Resolution3.5 - 4.0 Å
Structure data

14814

7znu

EMDB-14814, PDB-7znu:
cryo-EM structure of CGT ABC transporter in detergent micelle
Method: EM (single particle) / Resolution: 4.0 Å

14843

7zo8

EMDB-14843, PDB-7zo8:
cryo-EM structure of CGT ABC transporter in nanodisc apo state
Method: EM (single particle) / Resolution: 3.6 Å

14844

7zo9

EMDB-14844, PDB-7zo9:
cryo-EM structure of CGT ABC transporter in vanadate trapped state
Method: EM (single particle) / Resolution: 3.5 Å

14845

7zoa

EMDB-14845, PDB-7zoa:
cryo-EM structure of CGT ABC transporter in presence of CBG substrate
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-VO4:
VANADATE ION / Vanadate

ChemComp-PLC:
DIUNDECYL PHOSPHATIDYL CHOLINE / phospholipid*YM

Source
  • brucella abortus 2308 (bacteria)
KeywordsSUGAR BINDING PROTEIN / cyclic-beta-glucan / ABC transporter / CGT / Brucella

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