Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of dynein-dynactin on microtubules shows tandem adaptor binding.
Journal, issue, pages	Nature, Vol. 610, Issue 7930, Page 212-216, Year 2022
Publish date	Sep 7, 2022
Authors	Sami Chaaban / Andrew P Carter /
PubMed Abstract	Cytoplasmic dynein is a microtubule motor that is activated by its cofactor dynactin and a coiled-coil cargo adaptor. Up to two dynein dimers can be recruited per dynactin, and interactions between ...Cytoplasmic dynein is a microtubule motor that is activated by its cofactor dynactin and a coiled-coil cargo adaptor. Up to two dynein dimers can be recruited per dynactin, and interactions between them affect their combined motile behaviour. Different coiled-coil adaptors are linked to different cargos, and some share motifs known to contact sites on dynein and dynactin. There is limited structural information on how the resulting complex interacts with microtubules and how adaptors are recruited. Here we develop a cryo-electron microscopy processing pipeline to solve the high-resolution structure of dynein-dynactin and the adaptor BICDR1 bound to microtubules. This reveals the asymmetric interactions between neighbouring dynein motor domains and how they relate to motile behaviour. We found that two adaptors occupy the complex. Both adaptors make similar interactions with the dyneins but diverge in their contacts with each other and dynactin. Our structure has implications for the stability and stoichiometry of motor recruitment by cargos.
External links	Nature / PubMed:36071160 / PubMed Central
Methods	EM (single particle)
Resolution	3.3 - 20.0 Å
Structure data	14549 7z8f EMDB-14549, PDB-7z8f: Composite structure of dynein-dynactin-BICDR on microtubules Method: EM (single particle) / Resolution: 20.0 Å 14550 7z8g EMDB-14550, PDB-7z8g: Cytoplasmic dynein-1 motor domain Method: EM (single particle) / Resolution: 3.52 Å 14551 7z8h EMDB-14551, PDB-7z8h: Cytoplasmic dynein-1 motor domain AAA1, AAA2, and AAA3 subunits Method: EM (single particle) / Resolution: 3.41 Å 14552 7z8i EMDB-14552, PDB-7z8i: The barbed end complex of dynactin bound to BICDR1 and the cytoplasmic dynein tails (A2, B1, B2) Method: EM (single particle) / Resolution: 3.3 Å 14553 7z8j EMDB-14553, PDB-7z8j: Cytoplasmic dynein (A2) bound to BICDR1 Method: EM (single particle) / Resolution: 3.93 Å 14555 7z8k EMDB-14555, PDB-7z8k: Cytoplasmic dynein (A1) bound to BICDR1 Method: EM (single particle) / Resolution: 4.37 Å 14556 7z8l EMDB-14556, PDB-7z8l: Cytoplasmic dynein light intermediate chain (B1) bound to the motor domain (A2). Method: EM (single particle) / Resolution: 4.9 Å 14559 7z8m EMDB-14559, PDB-7z8m: The pointed end complex of dynactin bound to BICDR1 Method: EM (single particle) / Resolution: 3.37 Å EMDB-15396: Consensus map of dynein-dynactin-BICDR on microtubules Method: EM (single particle) / Resolution: 6.33 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM / Adenosine diphosphate ChemComp-MG: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate ChemComp-ZN: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM
Source	homo sapiens (human) sus scrofa (pig) mus musculus (house mouse) pig (pig)
Keywords	STRUCTURAL PROTEIN / Dynein / dynactin / cargo transport / activating adaptor / cytoskeleton