+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-15396 | ||||||||||||
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Title | Consensus map of dynein-dynactin-BICDR on microtubules | ||||||||||||
Map data | Consensus map of Dynein-Dynactin-BICDR on microtubules | ||||||||||||
Sample |
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Biological species | Homo sapiens (human) / Sus scrofa (pig) / Mus musculus (house mouse) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.33 Å | ||||||||||||
Authors | Chaaban S / Carter AP | ||||||||||||
Funding support | United Kingdom, European Union, 3 items
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Citation | Journal: Nature / Year: 2022 Title: Structure of dynein-dynactin on microtubules shows tandem adaptor binding. Authors: Sami Chaaban / Andrew P Carter / Abstract: Cytoplasmic dynein is a microtubule motor that is activated by its cofactor dynactin and a coiled-coil cargo adaptor. Up to two dynein dimers can be recruited per dynactin, and interactions between ...Cytoplasmic dynein is a microtubule motor that is activated by its cofactor dynactin and a coiled-coil cargo adaptor. Up to two dynein dimers can be recruited per dynactin, and interactions between them affect their combined motile behaviour. Different coiled-coil adaptors are linked to different cargos, and some share motifs known to contact sites on dynein and dynactin. There is limited structural information on how the resulting complex interacts with microtubules and how adaptors are recruited. Here we develop a cryo-electron microscopy processing pipeline to solve the high-resolution structure of dynein-dynactin and the adaptor BICDR1 bound to microtubules. This reveals the asymmetric interactions between neighbouring dynein motor domains and how they relate to motile behaviour. We found that two adaptors occupy the complex. Both adaptors make similar interactions with the dyneins but diverge in their contacts with each other and dynactin. Our structure has implications for the stability and stoichiometry of motor recruitment by cargos. | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_15396.map.gz | 202.1 MB | EMDB map data format | |
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Header (meta data) | emd-15396-v30.xml emd-15396.xml | 23.6 KB 23.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_15396_fsc.xml | 17.2 KB | Display | FSC data file |
Images | emd_15396.png | 75 KB | ||
Masks | emd_15396_msk_1.map | 216 MB | Mask map | |
Others | emd_15396_half_map_1.map.gz emd_15396_half_map_2.map.gz | 193.9 MB 193.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-15396 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15396 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_15396.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Consensus map of Dynein-Dynactin-BICDR on microtubules | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.489 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_15396_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: Half map 1 of Dynein-Dynactin-BICDR on microtubules
File | emd_15396_half_map_1.map | ||||||||||||
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Annotation | Half map 1 of Dynein-Dynactin-BICDR on microtubules | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2 of Dynein-Dynactin-BICDR on microtubules
File | emd_15396_half_map_2.map | ||||||||||||
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Annotation | Half map 2 of Dynein-Dynactin-BICDR on microtubules | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Complex of dynein, dynactin, and BICDR1 bound to microtubules
Entire | Name: Complex of dynein, dynactin, and BICDR1 bound to microtubules |
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Components |
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-Supramolecule #1: Complex of dynein, dynactin, and BICDR1 bound to microtubules
Supramolecule | Name: Complex of dynein, dynactin, and BICDR1 bound to microtubules type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#7 |
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Molecular weight | Theoretical: 4 MDa |
-Supramolecule #2: Dynein, cytoplasmic 1
Supramolecule | Name: Dynein, cytoplasmic 1 / type: organelle_or_cellular_component / ID: 2 / Parent: 1 / Macromolecule list: #13-#16 |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 1.4 MDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
-Supramolecule #3: Dynactin
Supramolecule | Name: Dynactin / type: organelle_or_cellular_component / ID: 3 / Parent: 1 / Macromolecule list: #1-#10 |
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Source (natural) | Organism: Sus scrofa (pig) |
Molecular weight | Theoretical: 1.1 MDa |
-Supramolecule #4: BICDR1
Supramolecule | Name: BICDR1 / type: organelle_or_cellular_component / ID: 4 / Parent: 1 / Macromolecule list: #11 |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 130 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.2 Component:
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 293.15 K / Instrument: FEI VITROBOT MARK IV / Details: 20 second incubation. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 81000 |
Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 14 / Number real images: 88715 / Average exposure time: 3.0 sec. / Average electron dose: 53.0 e/Å2 Details: Images were collected in movie-mode and fractionated into 53 movie frames |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: FLEXIBLE FIT |
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