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Structure paper

TitleMitoribosomal small subunit maturation involves formation of initiation-like complexes.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 119, Issue 3, Year 2022
Publish dateJan 18, 2022
AuthorsTea Lenarčič / Moritz Niemann / David J F Ramrath / Salvatore Calderaro / Timo Flügel / Martin Saurer / Marc Leibundgut / Daniel Boehringer / Céline Prange / Elke K Horn / André Schneider / Nenad Ban /
PubMed AbstractMitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different ...Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different organisms exhibit considerable structural variations, recent insights into mitoribosome assembly suggest that mitoribosome maturation follows common principles and involves a number of conserved assembly factors. To investigate the steps involved in the assembly of the mitoribosomal small subunit (mt-SSU) we determined the cryoelectron microscopy structures of middle and late assembly intermediates of the mitochondrial small subunit (mt-SSU) at 3.6- and 3.7-Å resolution, respectively. We identified five additional assembly factors that together with the mitochondrial initiation factor 2 (mt-IF-2) specifically interact with functionally important regions of the rRNA, including the decoding center, thereby preventing premature mRNA or large subunit binding. Structural comparison of assembly intermediates with mature mt-SSU combined with RNAi experiments suggests a noncanonical role of mt-IF-2 and a stepwise assembly process, where modular exchange of ribosomal proteins and assembly factors together with mt-IF-2 ensure proper 9S rRNA folding and protein maturation during the final steps of assembly.
External linksProc Natl Acad Sci U S A / PubMed:35042777 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 3.7 Å
Structure data

13660

7pua

EMDB-13660, PDB-7pua:
Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
Method: EM (single particle) / Resolution: 3.6 Å

13661

7pub

EMDB-13661, PDB-7pub:
Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-PM8:
S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE

ChemComp-ZN:
Unknown entry

ChemComp-FDA:
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-HOH:
WATER / Water

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM / Uridine triphosphate

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM / Flavin adenine dinucleotide

Source
  • trypanosoma brucei brucei (eukaryote)
KeywordsRIBOSOME / mitoribosome / assembly intermediate / Trypanosoma brucei / small subunit / mt-SSU / SSU

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