EMDB-13660: Middle assembly intermediate of the Trypanosoma brucei mitoriboso...

Basic information

• Entry: Database: EMDB / ID: EMD-13660
• Title: Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
• Map data: Middle assembly intermediate of T. brucei mitoribosome

Sample

• Complex: Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
  • RNA: x 2 types
  • Protein or peptide: x 79 types
• Ligand: x 8 types

Function / homology

Function:

modulation of formation of structure involved in a symbiotic process / mitochondrial mRNA editing complex / mitochondrial RNA processing / deoxyribodipyrimidine photo-lyase / thiosulfate sulfurtransferase activity / deoxyribodipyrimidine photo-lyase activity / kinetoplast / quorum sensing / mRNA stabilization / nuclear lumen / response to arsenic-containing substance / ciliary plasm / mitochondrial small ribosomal subunit / acyl binding / acyl carrier activity / mitochondrial respiratory chain complex I / translation initiation factor activity / fatty acid biosynthetic process / NAD binding / NADP binding / transferase activity / oxidoreductase activity / ribosome / mitochondrial matrix / structural constituent of ribosome / translation / mRNA binding / GTPase activity / GTP binding / mitochondrion / RNA binding / cytosol / cytoplasm

Domain/homology:

T-cell activation inhibitor, mitochondrial / Domain of unknown function DUF4460 / Domain of unknown function (DUF4460) / PPR repeat / Probable Zinc-ribbon domain / Pentacotripeptide-repeat region of PRORP / Probable Zinc-ribbon domain / Pentacotripeptide-repeat region of PRORP / ADP-ribosylation/Crystallin J1 superfamily / 3-hydroxyisobutyrate dehydrogenase-like, NAD-binding domain / Protein Fyv4 / NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase / IGR protein motif / IGR / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Phosphotyrosine protein phosphatase I / Phosphotyrosine protein phosphatase I superfamily / Low molecular weight phosphotyrosine protein phosphatase / Low molecular weight phosphatase family / Cryptochrome/DNA photolyase, FAD-binding domain / FAD binding domain of DNA photolyase / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / 6-phosphogluconate dehydrogenase, NADP-binding / NAD binding domain of 6-phosphogluconate dehydrogenase / Pentatricopeptide repeat domain / DNA photolyase, N-terminal / Cryptochrome/photolyase, N-terminal domain superfamily / DNA photolyase / Photolyase/cryptochrome alpha/beta domain profile. / Cryptochrome/DNA photolyase, FAD-binding domain-like superfamily / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Rhodanese-like domain / LysM domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / LysM domain / Rhodanese-like domain / 6-phosphogluconate dehydrogenase, domain 2 / Acyl transferase / Acyl transferase domain / Acyl transferase domain in polyketide synthase (PKS) enzymes. / Acyl transferase/acyl hydrolase/lysophospholipase / 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily / Acyl carrier protein (ACP) / Phosphopantetheine attachment site / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / Rossmann-like alpha/beta/alpha sandwich fold / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 superfamily / Ubiquitin family / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S5, C-terminal / Ribosomal protein S8 / Ribosomal protein S5, C-terminal domain / Ubiquitin-like domain / Ribosomal protein S9 / Ribosomal protein S17/S11 / Ribosomal protein S9/S16 / Ribosomal protein S17 / Ribosomal protein S11 superfamily / Ubiquitin domain profile. / Tetratricopeptide-like helical domain superfamily / Small GTP-binding protein domain / S15/NS1, RNA-binding / Translation protein, beta-barrel domain superfamily / Ribosomal protein S5 domain 2-type fold, subgroup / Ubiquitin-like domain superfamily / Ribosomal protein S5 domain 2-type fold / NAD(P)-binding domain superfamily / Nucleic acid-binding, OB-fold / P-loop containing nucleoside triphosphate hydrolase

Component:

Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / PPR_long domain-containing protein / Uncharacterized protein / Uncharacterized protein / Ubiquitin-like domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / LysM domain-containing protein / Uncharacterized protein / Translation initiation factor IF-2, putative / Uncharacterized protein / Uncharacterized protein / 30S Ribosomal protein S17, putative / Acyl transferase-like protein, putative / 30S ribosomal protein S8, putative / Uncharacterized protein / Uncharacterized protein / Ribosomal_S5_C domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / DNA photolyase, putative / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / Uncharacterized protein / Small ribosomal subunit protein mS41 / Uncharacterized protein / BRO1 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Target SNARE / Phosphotyrosine protein phosphatase I domain-containing protein / DUF4460 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S9 / Uncharacterized protein / Acyl carrier protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Uncharacterized protein / Uncharacterized protein / Probable Zinc-ribbon domain-containing protein / Succinate dehydrogenase assembly factor 3, mitochondrial / Uncharacterized protein / Small ribosomal subunit protein mS29 / Rhodanese domain-containing protein / Uncharacterized protein

• Biological species: Trypanosoma brucei brucei (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 3.6 Å
• Authors: Lenarcic T / Leibundgut M / Saurer M / Ramrath DJF / Fluegel T / Boehringer D / Ban N

Funding support

Switzerland, 3 items

Organization	Grant number	Country
Swiss National Science Foundation	31003A_182341	Switzerland
European Molecular Biology Organization (EMBO)	1074-2019	Switzerland
Swiss National Science Foundation	182880	Switzerland

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2022; Title: Mitoribosomal small subunit maturation involves formation of initiation-like complexes.; Authors: Tea Lenarčič / Moritz Niemann / David J F Ramrath / Salvatore Calderaro / Timo Flügel / Martin Saurer / Marc Leibundgut / Daniel Boehringer / Céline Prange / Elke K Horn / André Schneider / Nenad Ban / ; Abstract: Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different organisms exhibit considerable structural variations, recent insights into mitoribosome assembly suggest that mitoribosome maturation follows common principles and involves a number of conserved assembly factors. To investigate the steps involved in the assembly of the mitoribosomal small subunit (mt-SSU) we determined the cryoelectron microscopy structures of middle and late assembly intermediates of the mitochondrial small subunit (mt-SSU) at 3.6- and 3.7-Å resolution, respectively. We identified five additional assembly factors that together with the mitochondrial initiation factor 2 (mt-IF-2) specifically interact with functionally important regions of the rRNA, including the decoding center, thereby preventing premature mRNA or large subunit binding. Structural comparison of assembly intermediates with mature mt-SSU combined with RNAi experiments suggests a noncanonical role of mt-IF-2 and a stepwise assembly process, where modular exchange of ribosomal proteins and assembly factors together with mt-IF-2 ensure proper 9S rRNA folding and protein maturation during the final steps of assembly.

History

Deposition	Sep 29, 2021	-
Header (metadata) release	Mar 2, 2022	-
Map release	Mar 2, 2022	-
Update	Mar 2, 2022	-
Current status	Mar 2, 2022	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_13660.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Middle assembly intermediate of T. brucei mitoribosome
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.04 / Movie #1: 0.05
Minimum - Maximum	-0.12710251 - 0.26356667
Average (Standard dev.)	0.00086969795 (±0.009215639)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 500.4 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	360	360	360
origin x/y/z	0.000	0.000	0.000
length x/y/z	500.400	500.400	500.400
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	512	512	512
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	360	360	360
D min/max/mean	-0.127	0.264	0.001

Supplemental data

Sample components

Entire : Middle assembly intermediate of the Trypanosoma brucei mitoriboso...

• Entire: Name: Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit

Components

• Complex: Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
  • RNA: 9S rRNA
  • RNA: 9S rRNA
  • Protein or peptide: uS3m
  • Protein or peptide: Ribosomal_S5_C domain-containing protein
  • Protein or peptide: bS6m
  • Protein or peptide: 30S ribosomal protein S8, putativeRibosome
  • Protein or peptide: uS9m
  • Protein or peptide: LysM domain-containing protein
  • Protein or peptide: uS11m
  • Protein or peptide: uS12m
  • Protein or peptide: uS14m
  • Protein or peptide: uS15m
  • Protein or peptide: bS16m
  • Protein or peptide: 30S Ribosomal protein S17, putativeRibosome
  • Protein or peptide: bS18m
  • Protein or peptide: uS19m
  • Protein or peptide: bS21m
  • Protein or peptide: mS22
  • Protein or peptide: mS23
  • Protein or peptide: mS26
  • Protein or peptide: mS29
  • Protein or peptide: mS33
  • Protein or peptide: mS34
  • Protein or peptide: mS35
  • Protein or peptide: mS37
  • Protein or peptide: mS38
  • Protein or peptide: Protein FYV4, mitochondrial
  • Protein or peptide: mS49
  • Protein or peptide: mS50
  • Protein or peptide: mS51
  • Protein or peptide: mS52
  • Protein or peptide: mS53
  • Protein or peptide: mS54
  • Protein or peptide: mS55
  • Protein or peptide: mS56
  • Protein or peptide: mS57
  • Protein or peptide: mS58
  • Protein or peptide: mS59
  • Protein or peptide: mS62
  • Protein or peptide: mS63
  • Protein or peptide: mS65
  • Protein or peptide: Rhodanese domain-containing protein
  • Protein or peptide: Ubiquitin-like domain-containing protein
  • Protein or peptide: mS69
  • Protein or peptide: mS70
  • Protein or peptide: mS71
  • Protein or peptide: mS72
  • Protein or peptide: mS73
  • Protein or peptide: mS74
  • Protein or peptide: PPR_long domain-containing protein
  • Protein or peptide: mt-SAF3
  • Protein or peptide: mt-SAF5
  • Protein or peptide: DUF4460 domain-containing protein
  • Protein or peptide: mt-SAF7
  • Protein or peptide: mt-SAF9
  • Protein or peptide: mt-SAF21
  • Protein or peptide: mt-SAF22
  • Protein or peptide: mt-SAF23
  • Protein or peptide: LMWPc domain-containing protein
  • Protein or peptide: mt-SAF27
  • Protein or peptide: mt-SAF29
  • Protein or peptide: mt-SAF31
  • Protein or peptide: Acyl carrier protein
  • Protein or peptide: DUF4379 domain-containing protein
  • Protein or peptide: DNA photolyase, putative
  • Protein or peptide: Acyl transferase-like protein, putative
  • Protein or peptide: mt-SAF37
  • Protein or peptide: mt-SAF38
  • Protein or peptide: Translation initiation factor IF-2, putative
  • Protein or peptide: mt-SAF39
  • Protein or peptide: Unk8
  • Protein or peptide: UnkC
  • Protein or peptide: UnkD
  • Protein or peptide: UnkF
  • Protein or peptide: UnkG
  • Protein or peptide: UnkI
  • Protein or peptide: UnkK
  • Protein or peptide: Unk
  • Protein or peptide: UnkP
  • Protein or peptide: Unka
  • Protein or peptide: Unkg
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: PHOSPHATE IONPhosphate
• Ligand: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE
• Ligand: ZINC ION
• Ligand: DIHYDROFLAVINE-ADENINE DINUCLEOTIDE
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: water

Supramolecule #1: Middle assembly intermediate of the Trypanosoma brucei mitoriboso...

• Supramolecule: Name: Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#81
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)

Macromolecule #1: 9S rRNA

• Macromolecule: Name: 9S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 198.441406 KDa

Sequence

String:

UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA AUAAAAAAAA UAAUUAAUAU AUAUCAAUAA UAAAUUAAAU UAAUCUAUUA GUCAGAAAUG GAUGCCAGCC GU UGCGGUA AUUUCUAUGC UUUUAAAUAU UAUACAAUUA UCAUAUUAAA UUGUUAAGUG CUGAUUUAAC CAAUAAAAAU AUA AAUAAU UUUUAUUUGU UUUUAAACAC CAUUAGGUAU AUGCAAAUAU AAAAUUAUAG UAAUUAUAAA UUAUAUUAUA UUAU AUUUA UUCAUAUAAU UAAUAGGAUA AUAUUUGUAG UUUUUGAUAC CAUGAUAAGG AUUAUAAAUU GAAAGUGUUA AUAUC AUAA UCAAAAUUUA UUAUUUAUAU UAAAUAUGUA UGUGUAGAUA AAAUAAGAAA UUAAAAAGGU AUUGUUGCCC ACCAAU UUU UAUAAUAAAA AUAACGUGCA GUAAUUAAUA UAUUUAUAAA AAUAUAUUUU UUUUUUUU

Macromolecule #2: 9S rRNA

• Macromolecule: Name: 9S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 919.62 Da

Sequence

String:

AAU

Macromolecule #3: uS3m

• Macromolecule: Name: uS3m / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 9.07094 KDa

Sequence

String:

MFLIHFVHYK TILQKYTFKF KHIFLSIDKY NSLFFNISGI LIWLNIIHIN IILIKYSFFI LINNFEYLII LIST

Macromolecule #4: Ribosomal_S5_C domain-containing protein

• Macromolecule: Name: Ribosomal_S5_C domain-containing protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 50.147355 KDa

Sequence

String:

MFRLTAASQK NLNSWYTKGT MRGGVPRIYY AWMRPGSFTR RRFEKMRNPF VDLETGTSLY FRDTRDSAEA IAHAADSKGI KGMDNAIDL YNEYRIVPDL YPEGFQWKHK LNTEYNQWRS NTWLTPDLIP KEHRGRFLCN FQLNIVAYDM RVVKFSPKDH R QWIYCVLY VGSGKGIAGW GRAVAPSTQE AKKEAIREAF SNIIAVDLEQ EGPMYPVRVN ADGVRVLLYP ARRIVANFRV AD ILCAFGF QHAGCRINLK ATNNPKSPTH TVEGVFEAVK ALRSVSEIAA SRGKVPHSLI YNIYPYLEEI RRRKGMMAMH PPG KDGLLM PDRVVDNRLP DHLKRGYYDD VYWKDFFAGS DEHLNEPRMG LRGDEMRRRL EEAQTSPAPT TAKDTRRRTL EDVL KRLGK TTRDLGSIPI VNPRVDVGLP THIKRNYQLH

Macromolecule #5: bS6m

• Macromolecule: Name: bS6m / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 18.861543 KDa

Sequence

String:

MVFYSFVLVM KPRQRRFTSQ ALREIGVAVY SNGGLIRSIT NEGIMRPYSR FRDADNTPLT YARYIILQLD MGEEEMGKVD KIIREHQDV LMALKLNNLE RPVGIRSGNK ELQAAYFPLD TFTRLEEEIN WSPQTSADIY TQLEMNWKEF SRTRWSSFLR N

Macromolecule #6: 30S ribosomal protein S8, putative

• Macromolecule: Name: 30S ribosomal protein S8, putative / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 32.766459 KDa

Sequence

String:

MLRQSLLSLA KVYGAVPPLG AAQGHRLLHG KREREGSLFA VANDVKRDER LLRQQLNALL EEERMPMQAR LQRNEAVSSH EAVPQTPLV DLPGVERRRD LPADPITRLF FQHKGDHALY YGTYDKPSVQ DDDRVQIEKR VPRRREENLY TPIYDFCHRI R EATEQRKR FVVVPSTIET RGCARVMHDH GLVAGFRDFH NDRAFAVELK YFQGDSTINV IEPCSYDGRT EFEWSPKMMR RL LNTHGIH NRLVVYICRT ADNRIIDHIH AVKENIGGRG LMMVH

Macromolecule #7: uS9m

• Macromolecule: Name: uS9m / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 50.201016 KDa

Sequence

String:

MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE AQALVQRTLE CISRPVSAAS RQLSRTITAE AVGLDNAPGH YTNFLEWMGR LTETRAFKTE HALFEFSRRK FN RDDVRVM FENYRLMSKA TLLADSADSY SHFYTVLKDF ARKVAGEDSR HQIGVRIDEA EVDPETGIAV GRGCADGEKY HFT ALLREN RDHNGIITVM GKPLSLVLDN KAWLMEMVLM PFDEANLDYR DFDAHIVSEG HAMPSIANEI AAFALRMAVA NALV KLIPL TRIPLKKSGL LSVDRRRERG QFPGYLDGKK VKRRFAKR

Macromolecule #8: LysM domain-containing protein

• Macromolecule: Name: LysM domain-containing protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 94.321055 KDa

Sequence

String:

MVRRSHVVAY YWSRYRMPTQ MPKFDGPAPV AAPQSMNSTK TNEFIDPIDD KFPMSIRGPL VRPDVPEDQY VDSWYICTSM THHMGDYRP WSASAPPNAF RFRPFNEFDA KGREYVQYMR EFARFDPRKS RGNGQKGFPF RDAYLTKMNE ANQKTPPPTL E TIMDRAVR EHHQHARILS PLEVQRDVGR LEPIPSYAGK INADRSVFPF QWKTEDWYEY EVAKVRNRRF VFENTEEDGI RG SEVTYKI VLEGFWDHHV MKLAEDVCMF LKDVGRQIVE EKLVAVRRLL QGGAVDPELL AAFNCARAGP FGGLDEYDKE EVA NFLRSD LRRLEEQCLS VINRCNVPVP GATNIYDPHT SWPHVEKLEP WVRMAEFWTS SSDTSFTELE MSTAHYEFRK FFRV IICKL PFQSTEFEKR MYDIRHWLHR QTSCEFHTIY RRNVIHDSAV FPTEHDPATP TTHEHHRMFS FALDWQSAPV NRLST DTVH EGESWDAVAQ RLGCSVGELK DANAERETIE AGVVINVPVT ATRRLTSFGA TPLVLPLKTT SAKDGERIRT WEEAAA ILD CTVEELQQCN GHAALTYQKK ESEAGEFDSS VTELVAPLSC WTSTSESEFS PVERVHANDT LVAIARRLQC SEEALRA VN DGITDVSGLD FVRVPPEARR PRRLVEPQLR PQAATDALLA RTIAEEETFK LKSIPHLPQN AERFPHEYHT PTSRFPPT P SETPATQDWM AYTAKYLDKQ FTISAEPAPV YNVNKLWPMQ QIPGKVDQTP FEEDQTWLLH SIPVQQLEMH HHEKDLQDL PFINHEQFPR SLEWNAP

Macromolecule #9: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 37.92591 KDa

Sequence

String:

MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHA(UNK)S NDGAVPPPRV LGGDYFK TR FGYSLVKNSE MTQGPVDYSQ LDMWGEMPRY TSDMVFLYLV SRRRNTYAVA YTYEGKRILN TYTAGNRGLK GGDRGFRS E GSTDNGHQVT SMYLNDLLPK LREMRASEGR PMGRGEKVEL VVRVMGFYNG RQGAVRAVQD RANEFHVRYF EDITPFPLN GPKMPRGVFK

Macromolecule #10: uS12m

• Macromolecule: Name: uS12m / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 10.421635 KDa

Sequence

String:

LFFLRMWFLY GCCLRFVLFV LCYYMSPRLP SSGNRRVLYA VFYLYNFVWM LRCFFCCFIG LVMSLFIIEG GGFVDLPGVK YYTRIVS

Macromolecule #11: uS14m

• Macromolecule: Name: uS14m / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 19.900014 KDa

Sequence

String:

MFCFSRGLWM RHIGQDVPKR HTHFVLESRL MYEKSFRDCW LHSVCRAISQ LDEPLSKTVV GTHQKMLQRK VTCFQYNQYG LFKTPYYRL ANVDRYHAVQ GVAGTREWVP YVNVSYWTMN KMVRGGNLLV HRVHYTGWGT DSHLKKGGWE HRWNKVLQRN V LQYSRI

Macromolecule #12: uS15m

• Macromolecule: Name: uS15m / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 50.401191 KDa

Sequence

String:

MFVRSFCFLR KYGSKSKTPD LKTSFTKTTN FRKAEKISIT TTDGEIVKGR GKIRRRTHSA LKEAIGAMEH PAIWLWYPWR MNPEPPTPH MPQRRALKNV HGAVFNDLTP VQKKRQEQML YGVNIPETRQ MKFEEQHPLL AGALRKLEGQ PKGFPFWYRK Y PTRRHAYE YRFSIPVEML DGYNDDVKKA LSKGMMSIQE KQFAQEAMYM ERYAEHDFDT TSPAVLAVKR ALKCRVLRNH LL TNPHNNI IKTVLANTER KLNHALRRLR KVDFKKYWEI IRDHDVQDIL QPPNLVTYRQ GSYWKYDWNA GLAISTNLAD VMD PRGLNG CVETGRSRSE VARDLGLSYT RPLHENEKKQ LSHQAVYYER LAKFKMEQPE AARAMERERF VRKFSGMFVK MDIR SGAPD FPSTYRRLLG TKVVRWASKR HGPN

Macromolecule #13: bS16m

• Macromolecule: Name: bS16m / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 22.077805 KDa

Sequence

String:

MQVTETVLAR AVIKRRSPQL WGAPGAPIIR MRGHHVVWKF QSYDLVVEHT HKRRNSDIRL LHYLGKHCPH PQKSLWSPDT PVAQDRHLF MLTTVDIDAF KYWFGVKRCR LSMKPWALLA KAGLLPPSLT QNSKIMPKPL FDKESLMRYY LANRKDEDVM A REKYLNYE NSMVKTEEER AAERPVAPYL

Macromolecule #14: 30S Ribosomal protein S17, putative

• Macromolecule: Name: 30S Ribosomal protein S17, putative / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 35.632727 KDa

Sequence

String:

MLRRSLLAFP WWNFQTEHRQ RCVLMYGGAR TKNTHNANHR VFIKKYKRNA FPNRTRHHWA VSMTGVLSQR PRRMPWPYDL TSLIFNQPR QGSDKIGYVV GTSMLKTAVV ATNHMVYYPK FNQRVSRTKR FFAHDEDLAC VEGDLVHIKQ CRKISKYKHY Y VFSILEPN VEGRERLKLG LKAVPPPLFG YPVSRRIVKL NLTSTEGTQE KLAAAIQEHV QDAYRFSGPT PDQPRNRLAD PV TFEDANN MIAPNAPAAA ALDASDSPPL LDRGEYTEVE QDTRNKKGDD YWMNLQPKEK YDFKSFKKSP

Macromolecule #15: bS18m

• Macromolecule: Name: bS18m / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 36.988691 KDa

Sequence

String:

MNRTGGSIYA HALSQFAVCR QPWNEYIGLL TKQDSTPYHT EPQEKPAYRG RKQGREGWLF GQQVQLHYHR FPDEQLITNL SRWRTGETV GDIAMQQFRN AQPFDIEDKD PQGVQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVVKLR V AKAQAIRI GLYPRFGNPF WFRSQKFRPK AYQENYDPTT YSTKRTVEHF AYNWVQTDRI RRYFRELEAV HTSGSASARG SG GGTTAEH KQQDQFYSPE NQPISLHRNN ISYMEDVGRS VKNPTVPGLM STKGMKKKFH NLYSSTSTKR MGFSNPTLGI KKV

Macromolecule #16: uS19m

• Macromolecule: Name: uS19m / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.364604 KDa

Sequence

String:

MAFRNTFTTP GKFSTVSKNI VLLLIWRVKV FLRAEGFAHS LVMLPVSLYS KILLCDVKKK IVYFHCCTRK KSMLRRCPCV WPDGPTKSS VSIGTAFTLQ KRFLKIAKSA FGFYLARRGQ RKYPFLRRPH IKNTHSMNPS APYFWSFMTA KSQMAFLPEE N YITGDWTG KFFVSKRQVY TLQHATSGAK VRVKSFPSIF EFNSPSRWNI GKEMNTLTKP RMDLIDEQML TKKQRLDYVR AG LLPK

Macromolecule #17: bS21m

• Macromolecule: Name: bS21m / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 23.087889 KDa

Sequence

String:

MLHTTRLWLG GYMMYHRKAM GTMKYSKWKG AHGGISHFYG RTPMVEEVRP NEPITLVDRR IMHYVHHSRL RHFQLFRSYQ EKSNSTECK LREGEMLRRR WHRRLQKSFI AFMQFKTMKV LEDQARLVNT YGQAAVNAAL GDPWNATDNV ARERKSAAVR R QVRALPMV NVVPKHVATM KQIHNDRFNY RWRVN

Macromolecule #18: mS22

• Macromolecule: Name: mS22 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 73.064141 KDa

Sequence

String:

MLRRAYIQRR YPFNKRGPRE HKSWKHHVLT EPPKPLQWRD PKVWTRDLSV MKSFDAPQWD LWQSRPRSED MDEALQPFMD MPKSLKDRR YDIPWWANPF GAWYLQNILS LELLKLKSKT NAEKIATYRS YMRSLASGKD NTMSDDDVIR NIIKERWKTL E FGDRNAGY PCTFGDYIQF LNEWFKSLDE EGMQRLREHF DRRIRPLLAV MSPVDILWLE ALTQNSPHNK EQLQRKIAFQ TS LGTPEFF DMSKRLRYEI NEDYKVRDEL GPELFALWSK APERWPPERL SKMYGLDFTL VRKILVWHHF KACYDACVEP DWS LPKRLF ALEWIRDVRA RKHGLFYGKM RFAEQKITFY SDRFLFRDLV NRREASYANV WEMDDPYRFL QTEQDYEDYW GDNY DVYRR MFPEMIGRTG EPVQQYGQMP IWAGPHRQHA NKSEHNWMFA EIGVNVGHEA LKKLELDPTN EKRRRFVIRQ PDGTL RSAK MSEMRAWYWK EEWADFRFWA PQMEWGIENT PSQEQYQEHV PDTTDADFRK QRRIQSRPVK WFYESHYTRT GSFAGF QPL RFMQRRTERE VRWPDVINAA VQIQKRKPAA YIFKAIPEL

Macromolecule #19: mS23

• Macromolecule: Name: mS23 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 36.869605 KDa

Sequence

String:

MRQCVVRRYK MPKNMGVAPR FDTWNEKYEP WEHMKRMGRL VGTGFYIPPE WYNHFRMFPP INHNFQQEKT LNPHNASEPT QDDTSTLSP ERVALRDELA RKSRLVASEG MRYYNIFWVR KPLDTMEKEY YELKRRGVDH GEAIRKVLQG FYSGLAVKKR V AAIQAEEA KLTGRFITMR EATVVLGVLA KLHKEQLTPH QVSLLAKEQG ETTQSGAKLT AIVSRTQPHV NKEASSPATS EA VGSSTEE SLSADALASM LSEDGEQSAV GTRYQVEVKE TANDSVRQLR EKAEDQTGFP DWYTGESPTY SGTS(UNK)CSRD GFALMKANK

Macromolecule #20: mS26

• Macromolecule: Name: mS26 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 51.192793 KDa

Sequence

String:

MMRSSSFCRR QIRPYYNLPS KSEHGRKMTG FLTPYRHWMW KQNELWRNVH EAQFEHLRRV YKRQWLESFR VNADEYIYKY NITKAAQLA QWECEMKEQE KKRIEARQMM DGRQALKKKH LDLLREFHER QFFFWYERAS ERLQNMNLIN YVPHAQLREH I DKELDKYV AGKNEPYPLN FVGQMPFLED GDGNIVEVPE SLLSNHMAEH PDSTAKPHEP HTSSSISEAA AFEERMLRAM VS AKEEDLK EWLGDDSRAL SETIDDISRE EEEREADIRV ARSMEETDAE REVSRRAYIE R(UNK)KTGSRSIF RPPTVSEGA GGTPSAPAGD ANTPMRRRKK GKLDRVHALQ AHQDELLAKL SSQGLKE(UNK)VD ASSVPERGKI VQSRGRIRDK AVIPTH EVL MQKPELAAGS TPGARIQTKD MVDKMYHRGK YKKSGSGDKS DGEDL

Macromolecule #21: mS29

• Macromolecule: Name: mS29 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 57.048836 KDa

Sequence

String:

MRSKTLKKSL AAMYMRPPVT CYTDACEAPV AMWDGAIPLK ETRKLKNGVP VRTVSRTYSH PPQLTPTQLS FNDINSMYCV GNDELIQFF PEGLGGRVFQ TMPPGHPRGF LYRKETHLLN LFVDKVQHWH TKRSVLSSLT NGRTGFIVDG PTGCGKSALM C QVVHFARS RNIVTLYVPD AKVWTHGEWC WPSTILPGFF DAPDAARSFL KYFAVANRAT LTSWKLRCTP KDLPTEQGER QP QNLYELC EWGHRAVAPA SIDRQSVCVK FLMDELSEEK KLPVVIVVDG WNLFSHETHF RYPHPDFLRG LASFNESSTD IDL YPQELP RIPASRLSFV RGLNKMILSG DDPNKFFITC TTRDFKPFDG ISGFPNVETD RFANSLDEYA PYDPEKDSHF HPIQ IGNFD EYEYRSFLRF LINSGELAGL GWGPLWHASS DFERKLYKIG FLSGRNPQGV VDHYHQELVW RYDYQRTRQK QYLLK RRME GMSRGASSGA VGVR

Macromolecule #22: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 21.244104 KDa

Sequence

String:

MVLRRWFPLL GVHRVGYTHP STLPVPCAQR WDLRLARARI FQEYIEEKAP GAWQLEDERS MSPEFKTFTG YPMRDMRPGY GQNLPDYIM KKRLPNNTHY ELFARRDIPN EDNAMYGKYL YDMTVHGTSL PSTYRMHKDI NKAQRNDRKL SGNRFKVLCS S GAKNPPSG WEPIPDATEE EED

Macromolecule #23: mS34

• Macromolecule: Name: mS34 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 28.72818 KDa

Sequence

String:

MLRCARVALR ADPLNGGSSM TLGSKGSKLS PEPHRRRMPW TAAKEYVPGV VLNARDKMVL DGVQLLDIES IDRASQLDPL EVLRAVVAT REYNISTGKN IFQLASQATY NGRGQRFYRK EWQEGTYDKY VTLSAIDFDR DGNKGTAYGY ITFHGETTTR P VQVDFADV PGWYMDFVEE RAVPFTGIVP PPPSIGTDVP VDPHSYRLKA YPYYDAPNPP EFVERLLKDR GVLPDTPTET AD VDKDPTT SDGSVHYDGK

Macromolecule #24: mS35

• Macromolecule: Name: mS35 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 98.277633 KDa

Sequence

String:

MRRWHRSLNP ALFGERGSGH YFSTTRARLQ LQAQDRIICG THGAGIVAHQ TAVNSSDNPM LSSVTTAAPA ADLSRTKPHE GTGTSERDP YIRTLHNQRS AAPESSVSQS HTVNAPTVDE CEMLAERWGT MNYWHNDTFP RLVVFLKKLL VPDVSPLSPT A ESLLSMFE KVVIPKLTSD EEDRRKLVSL WSETTLQAEA AVTKFLFQRG SFESMLHRII TDALEKMSTL ALGGQEGNLA LE ALKRQTL FKRNDYIQKR LIDVVSNSAY LGYGDSVWQV FFAAVEANEE NLLSDRATTD AIRAAWEGVM REDVVRLPDV TGV VALYLT LVCIRESGRL VPEELKELSS GLEDGVRPGV RKLQQYPLIF LHPTVKRRFV VKAVAEILHN SSSNAFSNML RENG LHDTA REVALCEAMN RNKELAEGDV GDAVGRFVSK GEVKTLLSSL VSGTDAVVRD AVAGIFGIGT TITIDWDAVM QNVDW SNNW QRLATALLSN SAVLSAIVKL VKNAIGAKGM SKHLFTDEYA DQLQLILDAR EERAASRKQR IENIAQELSS FERVDL SCD LLRKLGVDMT ELDTAAAATR NMNVVQRPCI EDGLLSLVLE AVTKRHPNWV KAGVIQTTLK DPFDALRWMM HIFIRLS YV PHAGAATIAR LSRRRIGPIG LEPHQFNVPA ELGFVEQYDN LQYKRYDWQG WYQRMLDVHN RNVSLRCRIC DLQRLDGN G VQFVDMQTER RLRILAQHRV GMGVLKLDAD KYEDQADNVT FGTTKLSELL ADARKAQLGE EYWPSVELKV RKPSGQSKA HYSLIDNERI EKRSGELYEK YRDAKKRSLF VTPMETWLEV KGMQVRKSVD NADEDGYTLD ALQDMMDGDD GDKV

Macromolecule #25: mS37

• Macromolecule: Name: mS37 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 24.757896 KDa

Sequence

String:

MKSSDIFFAY RLTPVVFKSR QHDSGVNQYG LKPTNAYDYI NPTNLINFGR GTTFDNLGVR RAGRGEIDSS PSHSGSPVFT QAKLIGLSG EEQLTMCQSE TMALRLCMAK AGKETCERES RALDSCLGRV GHLRRAMSEA CWEFNDWFIQ NVSDNHTKPF Q HRPHDWRH FYAQEKLVRE RQQNGHAYGR RPKQFSFGAR YVKTDGYGKR PRLPYNK

Macromolecule #26: mS38

• Macromolecule: Name: mS38 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 28.2406 KDa

Sequence

String:

MRAGCVACSR IPKLGIAALG STTTSMQATC QETQELRCAT TQMAPAVIFP LPPPLRGSYI DRKPTAASFN EHHATASFRH HMIASADVS HRPRHFYFAS GTRNDTGTRS VKEGERKQLK QQTNVEVDSV AYGDQLDELS ARWAAKFYGQ VTFGPRNYPY P SSRWLARR FQMKKHRIIK RFRFRRYKLA AVANLPFAKM IRVGMLPELK SSKTKRGDVV DPTLSGQLVS AVKNTGEKRK GQ RTRPKSK YQV

Macromolecule #27: Protein FYV4, mitochondrial

• Macromolecule: Name: Protein FYV4, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 22.421469 KDa

Sequence

String:

MLKRTLTVLD QTYGPHKSYK YTYMPDPRKL APIETTQRSE IVPQSIRPPT SYVPNHETFL ERADIHRLRP TSDFKASFKD WNDLFTCDK RQLRVRGIPR MTRDAIRTAV QAFQNGNPPE RFDTKEEWLY YKQFKTVDYS YRVIPELPEK YRPHQSGIDQ A PLPDYREI NKMPQWAECE ERRQSKKTV

Macromolecule #28: mS49

• Macromolecule: Name: mS49 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 137.234125 KDa

Sequence

String:

MIRRRVCDGA RLAPNIRATF SAVRYQSGLH TFIRDSKPSN FSSVRRSENA NGDATASPGA TAGENPASSG DWASHMQREL FGEVDPLGG QAHKDYYRDV TRGYSPQYAP RNFANGGAVA YPHIQSPYEY EEAAHRRVWL DHDVDRMREE FTQHRASLRS L ASAQEREE LLRSRAAEYQ VANTVHESES VHPIQQLYNS GGTSRSALKQ QAVADRYSIA EQHSPLPLTT GVDRDALDEA QR TKDRILN DSFTAENLLI THGLREKEKH DFTILQRTVR IPFQGYDMDR FLAQQKGTPY GAQQLPPNVV PSSMEEAQRT LRG SSATAT PLVDAVAQKV YARNTVVDRP AIGEQLTEQI INIMRASRTT AEQQREEERA QRFGLGRQGA LVQDGGPDQR TLKK HTNDE RIVDAMLFQQ NAYRKTPTDE HWNPYIRRST ENGVGHLLQN KFDIMRREDR LSKGEQDLTE RNTIHYGVPI QQIVD EFVF RHRNARGERP LDYFKPFPNF RALRLNRMYR DVEGFSLMKQ RPEFLEWELF TRYRQHHQQR RRLALLHGLE PVANET AQE RDTRRHRLDE ICERTPFDER EMRVNDDEMR VSVETLRSWF GVYMLPSPTV VNAVLGGSAS VNLHLYHLAD EMGTADT RE HVLSSRYLNR LLLLESYQNR VGRGFMNHVV GRAPEPVVPH EQPQEVLRHF SAEERAMYEQ HVKEQTSRQL GEWERAMK R RRWLTDHQQY GHVVSHGLET SVVDLSHTET GAVLTVSTKA YEQEIEAVRM KTNATIKVDG MVYNLLPNSE RRVVPLTVQ LDSGEKIDMT SEDFDRCELE AFPRNLNHAL NYGIANYAYN RGNYVETQDS IWEEQTASGQ EGWSPATHAD GLREGLPVRA RRPIFSSSA EQRIAGGPQR AVIIQYHHQP FFNPEPRLVK VAFQCDGTIM EVPISDVMIW QRRYHGPERT VGDESRRYNP A AMRRYVDV TDPFNEKTSN TEHFLDKYEP KRNADTVADK YRTTKQITEI DKWTRYDSAR ADNYRPLSIS HRRDYIRMGY IP RYTPWEW IAIQEADQPL IAEQIRQDNI GTSYFFSLNR YWRYKASPHG YIRHFENEVR DLLQYVDGVT PWKQAQKIRT YWE VRSHHP MPQFNRPEVA MHRNTVGLLP AHMWETDKKT GKVKSVKDSV RDYQTKTPYP KWVQL

Macromolecule #29: mS50

• Macromolecule: Name: mS50 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 131.71925 KDa

Sequence

String:

MFRVRRLAGF IQCSPCGKMG PLKQQTRRYT PIWKSDPAVD NVAPLRDEDE RRALWAEVGP ISDVGSAVTA WIRFGNDPVL HTAVPTMLG GKFRNQQREK ESLLPNSSSP FAYVEDYMGT NLVFGSPVHA KESAAVWATY FERRYASRLR LSRRTVANYV G LINSPEVF DDESDRPETR WSQDTFFREC AYLSEKFLKE KVSNMQQFEA ALKRASPEAY LAFFDAFQQQ TQTQIPLPSP SV WHYEGER RKQWAEKFIS ISHKAQAFFK DVLSEDVKKY QEVPGKLLQK VKPVLADVGK ILVKRHERWL KGRVWTSLTE EER EAYCMK EVKRQQMQVE DGEFDPMMED DVDDTELEEW QREHDAIMKL MNSPIDGLHF TTLELWLHTM RCEELETEHI YTSA RVRAI QVAARKKLYD TTSYEEVIQA VVESIARGTL DLGAGVLRPH FNEVWCQLNY AKFGSSTITQ HTTTSRRQLL FFHAG SLKD IAATATLYYA TKPLSNSLDY ASPYKYRRSL ITLCSNYGVE TAYTTQRPLL RSAANLARAE DLIHAVVTAA AQPFGE RRR AATRDLHMEF QRLAVPVERV IVANPVSALL ESGADPDEKP VEGEKVNMWP LGAKRVVLYK WSAPNVEKLK AMESDAA SA VSGSSLTAKR LREIQELKRR GFLEVSLWRR VTAQERKQRN EIVEAKKKQV EEVVRTVPSL AHLHQYATSL YSRIEERV A FPTETSTVTE TTNMKEETNK PLEDSEWEFA VLLDDRVLLN KEESVELYLP YRDANGELLA QGEYRALVRA FDLEANPNL HPAYCSVGYS ESFQVFDALP QLIAQFFRVK DATAEAAGVT HIPAADFTPF CAFLRDAGLD VPLRCEFEAG QAVTTDGDVY MDYFLQLLR GEAFHQSHAQ AGLTEAQRAI EPLCRAHWVV HHPGADESEW ATARRSVLDH AMQHEREWWF PNEMLDVKDV V TGSTNGLT PQMYPAAVRY GVELCTVLTA EGKFVDERGS GLSARCVVNG TGAAESVVFD TANCNGTNTT SVEDALRVAH GA LRSAQDR HNTLAAFRLG PLSKQSQVLL FCGVNAYEFG GKYARTYAYA FEKAKKELEA TAASGFMAPS LSHEDTERLS DQP TTSPSV DRFASTTHPE QRKAQFVPRV GPGSTPLEDP AADQKSEWS

Macromolecule #30: mS51

• Macromolecule: Name: mS51 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 93.661383 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)FV FRDPSL(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)H ANTQYQHAWP LLSHDDLGNS DQSNNTKNIM YSMYMPKRNK GTAPWFRGAD TYSVKYCEQG RYEYQRYLM INRFPSEYKK HFLSFLSNIR MSSGSATIPQ EALHWLLRMI VDNFNPQHVH YIAAMKTLQS AGELDMARDV WKIMERQQTW PCTATICAY LDVCVEAGEK TWAMEAWNRY CTELKFLEPG EVDPKPISRV PFSLTREELL YLPKWKKHFD HDPNLDVMDL N RFNRTREV YLRMAQVMLA GGERNAFQHF FTKLEEAMLN KPTPVPEPPN PHLVRRPRWA PYEHCKSVHH SPWRLQNNGR AL ALGPPVT IEDEMQSRFF SNDQFLVHSV KEVLRIVLQE HKRAHPTECT RCKTEAFFYK TKDADETLKF CDDLIERLFA SLG VRLSNL NTSSLLSTIL EVFRVVGKES GAALLQRANE FLERKASLGD AEGSRENLTA SNYLQVLSGF ADESAFVYNT KKDG TCQYK TGFDPRTTMR HLADVVQEIA GNPHVTWAAD MHLQVVETMV GCGTMKANDY FVRNVLRQFS WDSRFLEALY VEYRR QDDV DMWAELTKRA LVWTARYNAP ASERLRRLIE DDYDTIRVQT RTFRELAVFQ FRDVEERRHS RDVVNELPNP WYDYVA HAL PFPDRDAGYP DEYGDLGQWR APGGPGSPVR GPGYYAPPME GEHQRGYTAE WRDLRNPMRP PEFPTPWERK YRQYARG QH PSYDMVYAGP MPEIFPMRRD FRKPTRWDFH DIEKQGKYRT SGPY

Macromolecule #31: mS52

• Macromolecule: Name: mS52 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 84.938164 KDa

Sequence

String:

MRRCSFASTC RYRSADDVPL GLGSAYVWTC RNITSRGQFN PIHNFSYAME RGVRARDVKA FEKLITNPGP LRVAYTPDYL DWLHRCYKA KGTYMDARAV AEKKFNGNIV SSELSAAVNR REGKRGDTRG DTVDNDHHNL PGAPPPGMFL RPAHSFRRLA G ELKRRRAQ SILDEVARAQ GMLDLFERQP HFPAIHIDRC SRFHLVELFK EMVLERSLDS NMIWEKALLY RAILSERKPS YP TSFHYIF TAVEDTVFAP TISTPMEMAG SGGEGHDRSS HPLAAKCPTL EAYYYYVYLV KKYYIDNAVE AHVVLRCHRE PNA ADLLFS NPPPKDDTEI MKAVELLRNA DIQRGVAAAA AVSDPTLPPG GEGSVIGNSD N(UNK)KNSEK(UNK)SE GSRGRP ARP PVLPGAYPPI DMLWRCEENL PLLKVLLFGE FNLIVSENPF VKFPSAHGFL TRPYSTDSSR TLADGMSLAN VMAEKRG HL LPSLPRNTAT SIDARAQDIR RLQQKHHRDD IVSFQKLLRS THAEDSPSAF SSYSDWSYFN PRAVRAEERD RLTRKAVE A LKLYDSATND IYRHSFEDVQ ACHTQRVTER DRTMPPYLPT LPHFVAIIKK DPHISFLLHI GLPDRNSSEE GSAKHKELE KRIYYLARAL YHTALEYHNE TVRRVNRQKV NVAASLLDNF VEQEWTTILR DKHDVTDVTK TLNDTQNDKK QLARRLGRYM LFANRSLDD TGFPTDARAD DYTRWMAPPS VGKVSL

Macromolecule #32: mS53

• Macromolecule: Name: mS53 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 75.338914 KDa

Sequence

String:

MSVSGVFSKG RGIGHEATTS ILRYIPRARV PWQPSRFGRE NLTAADMARL WSRGRYRDGP GNYNSGYCTE RTHVLEENTV SIIPRRELE KYMPDITIGP KALVTPVSLM NARNGHRVTH DLLHSYDPHI GRLGKPAVVD HDNITVEDPN RVGLNAATLD C RGRIYRWL RRGPFFQVDN YFRRSVKLNR DGTLPTDFVH EAPLMRKIIR LAHRGHLKAA CEEYRRVTTV PPVEVYRALT AC CVPGAKL ADAVSIFEDG DSKLFYVSRD GEVLHNLMRC AIAARHRARI MWVYNVMRGR FYENVVVRAE VDLIWRYRIA MIA LEYLLD HECAEEAAAI YSYLVEEELL RCDVHVRVGL HMREAIAAGK PITLNNDVMN ATSLVRDATA VAPEVARELQ RRHA QTLQN NAVEAVGAEN VREGSAPWSI LGPLTAIGPT AEDTMVWLQQ HYGDVDVMSI MRWARFRKGK DLMAKDRPQY LARAA AWIE LLSKRNREME EVPLTYMRKS KPLVLDTNSN VRVAWQTPLM RSGGPPRLLA REEGYVFHHS NSSRFVEETY RHPGES LQS RYLALQPLHT EVSAKEDFQR LYYQAQKHHK QQERLLPVST AAIPSRIVHH SVMSALHGVS GKGEPANRSL FT(UNK) NKSNDS HSNTGVASGT SACTDGAGSA TPEF

Macromolecule #33: mS54

• Macromolecule: Name: mS54 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 72.046031 KDa

Sequence

String:

MFRRAIPLLS ANIPRSVWDP AQHNPNWSDS YGHDITNRRA WPARKWTVGL EPCTPREWLQ FSHRNLAYAY NGALRACHSL PSMLLLYKE MKQRGVKVDV DTMNVLLTRA ARHEHIQVDD VFLLFDELVA LGARPDLAAA ETLHTVLSHS ASMPEEWREA R RLQLVELY NNLAMEEVER LAPHRADRLL KEQMKRFRGN LQQLGSGLRP TVYCRYLHTT HTAAVLLEEV HNFLWELVPN DH PAMEIPA LQLRVPFVAS VLRRPSVNPG VSLASVSRAE FGDTDVCAVF LAAAERMVDA DFDDQRPVSE RRLFLSLLTM ISY SGVLYT SDLMAQLMEM VKYSNNDETR DSDAQRVLRY ALRGSSAAQD SASRTLWHSV EKVADCRVVG RYIGARNPWN PIRV CFDEQ GVFKAYPIST TTTTREVSPP EGNGAVTQEQ RASCVEGRTL EALNMRWDDV RRLIECTGVL VTPPSERCPQ QQKME VFTG MAVYLRTVAT GRRYEGGEDV LSDGAVATSS CEQRRRGTLF AEGYDFDVWV RLFSLVQEVR HDMEKFMADH TLQCVE PEF ECWEALLVTL RCALDFCVVQ MQGGGARGTE REVVERLFRD VVALREELIE ESRTRFGGRM RVLWLQEA

Macromolecule #34: mS55

• Macromolecule: Name: mS55 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 66.67668 KDa

Sequence

String:

MLSQNVAKTT VPSYYMIRTN LPHRKPQNQW EGVYYYSGIT KRQRHLILLH RKREREAHMR SFNISRASVL QRLEQLSGDR KQESLPPHV RLDLAVRLAQ HGLYQQATPI VDELHHQKAL HAGHYALLIN ALACPRLGQR ILHCDAQCDP ALTYKLLGDE N GEERAQEA YRWFDLALTS LAVDCGGRTQ PSHFVPYLPQ GTAAASHITN ALMRTLLTCG YTHVAAIPDS VYDRMGSMGI SP TISTYEL VMLALSLQGN MVEAESILSF LRSHHSEHIT VESFNALLLG HREARQFDCC DAIWQELVDR RWPRASPLTA ELY LRSIMD HANTPTSEPL QSFANINVVE KKKVPLVLAQ MDELGVPRTH LSRVLMDEVE DSLRKFQTYR SRFYEWGRAV KQFD FIEFR RRNGWLYDLH LMKCTTKQVG PLRDFNDPDA VQGAVATAEI PAFFNERPAW ERPPLEETLY VTTNKERYDD VRGGD IYYD DTRGLHDRSP TWMNEVPETR YDRLYGVNHP DIAKIGIRRH LNVEYVNRKE VVERDAALMK KTLSSGRRLR HRVESS RTH RNAGSLSGIS STAGGGSR

Macromolecule #35: mS56

• Macromolecule: Name: mS56 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 47.306836 KDa

Sequence

String:

MRKFCHFMAN CWNSARSHAT YGAVPLTHSQ VTSVYATDGG KVDELGLLEL VEERIFSWKL NKWEMRIPPN LPNDQKELIR QEQENLKQI LSEWRKCFGA LNADILQISS LTGVPKDVVR EKNRTWLQEE VAKLRWMGEV NKAALLRDAF MRLEAFGSRD F MFMERLCC IYGLARQGTF DEAFTNYITE DPVTNDIFVD ERNPFKELVA HIVRNYSQID IIYDFLGFNY SEGYRSSLRR YM EYLQCKT AENVRASGRL VTGDKGEHNI LFDYCVSRES LVSGDSCQGI IDFLYINGND VTLIIIASDN PWLRNRQLPH RRQ MEGIAR RVCFVLGIPP SEVRIRNLLL PPTYLDKGSI VRLNDIVFRL SNEQSNLLIP WLTNYNKELD PKDVDYTALA KTTN EEEWL TL

Macromolecule #36: mS57

• Macromolecule: Name: mS57 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 45.956293 KDa

Sequence

String:

MFRSTRPRSV GYTPVNPDTS PMVAYSQYHW HYNLPQGMER PHSVNRTFAA PFQSNHSLVN KYRGVWIEFD MHPAFSVALE PQLRKLPRG RTLPKTPAEE VIADYTALAP LVDDEKTRDL WLAKVFQHCA FQRCGGAMEL WERYCHQRFT AEGATAKPPL S LVKSVLFY CNKTDNSGWR ALFDRCLKDG WNYTPLFDTA QWSFMLKSIG RMGDEDGVRA VLEEMLDVQA DLDRVEARSV VI ALNAVTN ADVYEFVKKY LFNFGERKVK FLRTTYSDLR GHGAGKLRIP LKENDNMYYH VCWHSSIRSP RQFSPRQLYF DYT PSTLGS SSHNPNAKID DIVKDKIEKW KAEGLLPEDY VHEDRVYDRS AAFKNVARQE KWKKMPKILK SKRMGYTGDP

Macromolecule #37: mS58

• Macromolecule: Name: mS58 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 35.651918 KDa

Sequence

String:

MSFRYTNNLI GALKHRLLLE SSYREIASRK FIGNCRGVEV VCSGYGTVLA VQLTDKAVWE SFYRKGGRPT VSGGGDVSGD AETGTQGSA TTTGATGDLD LDKLAESIKT ALWDATRKIR SAKEAALHRS LSHNTRMRAS ADLKHWYEED ANTLRPLAFE A LKHEAATP WMQLVQHGKK EEAAALLKEF EQKGDAAEAT PTRVKDDRVK GTRTELSNRE QPPLASTLKA EDSNPATIPI GS VHPLFTP ALVQIEEAGG GSVSNEAVCR AQLWELSRDE QLFWERVELI RKGQVASIGS SHKRGYADEA AFAKDDTEEK VQL RFTQ

Macromolecule #38: mS59

• Macromolecule: Name: mS59 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 35.395676 KDa

Sequence

String:

MRCSCAFLDK SVFAAKRRVI VPIHPTPNFP AHFIKSAFTT DPLKEKQKAR FSSGGEAMRE VQDIPKNLEG ERSRRDLASR GDTEFQALV EFIEGASYDQ LISGRRFKKV YDVLSENDDM FIWLCHTAMS VLNPGDMRSR LIYNHLRILA ESVASGEMTQ R TAFRFFES AVRSPAYREI AKRQLEGGAA TRLAGISAAA DVMRRMGLTR RPMSSYFELY QRIVERSEAM TPWGFPPLFQ FE ERLSLEP RLKFFSRASQ QSLERRRRGH VMTPHTTLHG RRIFWIPPTW NRAGRFLGPH VTLYPGMTPD

Macromolecule #39: mS62

• Macromolecule: Name: mS62 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 32.0681 KDa

Sequence

String:

MRRFCTARVS SYVKRAPLLL PRGGRRWRSG AGTTADGGGE KEYIADSFES TAGSNAYAAM ELAAEARREI HELWLSAETA LEREKRVQQ VAALIEKYKL DPSTPREADV SRGLGDAFDR LLLLCLPLGK TDAKGTDNLE RLMHLAGRNG RELSVRTIQH L FARTDSFA EALAVFYTMR RCHVAMNMEA YYSMLYSLQR LEEEGWGQHF RNEYEENGAP SEQAMDFIVK GISNALLPEN KP WLGRVMF QDRNVPDRRY DTRDFDELDT AWTQRYKSGT PAGAH

Macromolecule #40: mS63

• Macromolecule: Name: mS63 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 31.922178 KDa

Sequence

String:

MLHGTPVRRA SLRYRRPYWM MFLKGVDNWK IYTVIQQPDH QRTEMLYQAW LGGLDRPYTR PKCMANQPLW LSKKRHMLRK ERLDGPETP LEKYVLEWHK KFHSFQGTER PTPDDLHTAL DLVERPLDLS YALQLLGQCR NLNNIRFAKE TFLVFLEACL R VGRRDCAE YALEHAEPLG FWFIDEDHRR YLQGEQTWYK LSPLDNLYYP VEENAKLNEG RKPITRLSPA TESPGSGTAI SD GEPSTDV EGETTVDDEI AQLEAELAAL EREGGGK

Macromolecule #41: mS65

• Macromolecule: Name: mS65 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 30.617381 KDa

Sequence

String:

MFSESLCILS RRFRYNTKFP ALVSYNKLPW EVVNHETPQF HMHVAPHYEQ LLTLAASSPV PHIIGSKHID VPREHRLRLL PGMLYLLDG DTLPGEFTIN RVLDPTALQY YGRLSSQIVT VEAVRMLVPD DLRLLCNCIT FKGPLHLPVA PYASLASLRG A SQGGTTGS ETGSNCFTLY HFVRPNRPPK ELQLEKYYIH APCVAPLSEF ASNSDERGNW RPRLQAPKRT RRATPLPAYR PP QSYLMGL AERLAVVPGG CFGRRSLMWG HWF

Macromolecule #42: Rhodanese domain-containing protein

• Macromolecule: Name: Rhodanese domain-containing protein / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 30.096051 KDa

Sequence

String:

MLVSYLRLQA DRRTRHHMMV DGQINPREWF NWHPKEYQPW YFDRHKFLYE QRQYAQFHHL LCHQVYLKDM LHLMRYPEPY THIIDCRTS TQKMHRWVPH SLWLPRDEVE YALQLSADEF LDMYGFRKPD KSDDVILLSH NGIYSEQAGW EWKKQFFQHV Y NYRGGTNE LFGESYSDMN VSDVLSPWKG PFPQSGVFVD KWSKRKVLTR TGPFDRQYEM QDFALPDLEL EKARHPEEGP RQ NMPFGLQ

Macromolecule #43: Ubiquitin-like domain-containing protein

• Macromolecule: Name: Ubiquitin-like domain-containing protein / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 26.472807 KDa

Sequence

String:

MRRGALSLLK AGLLGHYQQE AFEARKRFEE STTYPGPIRA ATPGDTRFYS GSLESILHDT DRHYWRAVTD DPRVQHLIPL RIRFKIFTW VTSGWEQRMQ VVQIMAPKDS TIAQVKDLVI VENQSPYLCV SSFHLAIDGK ELDPQKTLGE YGITEQSQID A IEQNDHLL HRDDERPRDW TVDEITAEDV KRSPYKEMEM QPLQNLAPRY EARPKGYFGR TYYSGMKQSS

Macromolecule #44: mS69

• Macromolecule: Name: mS69 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 21.589732 KDa

Sequence

String:

MRRVRDGLFL SHPSSALQCS VAVVSTGEFD HPPFQFRQRH TFNTTPLHDA NRFGGRTAYL REIGPVNIKK QGRRFKKDPR TVQFNVDVW CAQQTLRKRW KQRDWEVIEI PFRLVPREQQ RVIPELYTDI PQMTDPARND FSNIRNKVYD REELQGVLFP A AGAMLYPP LQRVDKQAMT LDKYL

Macromolecule #45: mS70

• Macromolecule: Name: mS70 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 21.247398 KDa

Sequence

String:

MLRFTHRALT ATPERFSVLG TTHPKPKRTG FGRNNKMRSK PSDNVAWYDK GPVEWLPRPV RLTYDHLDQL QQWTMRATLD GRTEEFNRI RDLHREWSQH PLMPVLGDVE PKFPLNLFKQ NHRAKKRFLV RWHKANTPAN WLWMPRGPTV VTPLHRTNPT Q YPENWKQM VQRKSGTGTP S

Macromolecule #46: mS71

• Macromolecule: Name: mS71 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 20.093227 KDa

Sequence

String:

MFHRAFVSSS DLTGCTIALS SVCTQKRYWA KPKKRPKVGQ GFHEKAQKWR EEYLLDRHRA LADSLRAYVE FSTSKRVEPW DARFKPFDR IEKDGVYVLM RYMMEEKLQL CNYHHRPVKR LFCNIGLMGP QITTKARWKP YRFATNPAGT SKAERMYQRD K TVYTHGHN D

Macromolecule #47: mS72

• Macromolecule: Name: mS72 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 19.340352 KDa

Sequence

String:

MLRSTLTSLN TFLTSSVATP PISVIRTGPK WWAHPERMVR QKLMYFTLGV DQLPLRRTAV IQRDLQRFHM CKPPPRVGDS TGYKRSRAA QLNTWYRRIQ YQEYHMQHLF TRHVWGLLRV YPGNTTKIQG KADDGYVGYD SVPFHRYNRA PLPFPARELY E RRK

Macromolecule #48: mS73

• Macromolecule: Name: mS73 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 11.360885 KDa

Sequence

String:

MLRRSILFRM KYADLELTTR GEFPHGMKEP AFVKKLDQNI PWYFSTYRSM YHWPITGDNW SDLNEAEKHH DLHMFYTLAW WKLGEGIFG VDEDS

Macromolecule #49: mS74

• Macromolecule: Name: mS74 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 8.203408 KDa

Sequence

String:

MFSLTQTWLI AHWYCGHKFR HRFMRDKRFH PSLQASHDAR NRFSKRRHFK TNRWNYQQAY RDMP

Macromolecule #50: PPR_long domain-containing protein

• Macromolecule: Name: PPR_long domain-containing protein / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 116.189547 KDa

Sequence

String:

MLTPTRRLPK ALSPYAQALR HVALRGATAF GPGAKEMELD MLRKGTLPAD YRPPVQGRWD DTIERWAYAW QFPAEEEQDD ITKSVERNA SGMQALLEIG NKLLRSPPSP EPLSGKASKL YPPVGDMSSG NTALLPPNPT YDVLEQDVSE LMAEDAVVVT S QPRLRAEE LSAKYNVPMA YIDDSSEASN ASKSLALVME DVGLEFTEDG LTVVISALSR QGYGTIGRAI FDFASAMGLG PS AEMYRAL MKYASRRGDV NESMALIEEM KGNGITPRIG NWHELMYTFY KAKDYPAVSQ IVDNMKMYAN IEPNEVTFVL QLK ALAKDN SQLNSLPEAI QLFDQMENVY GFIASRPHYD AMMFHLSQSP RPEMRLRCEE LAHKMELMGI VWNANTYLNL IRSA QVVGD VAAVEKYLSR MREEGIPASI GHLTWAVQAH VQSMIRIDYD ALKEKDESPL PTWLEHLETC FGIYELVVRR GWVMQ LPFV NALLRLTCQA TILSMERTPD EAETIGRFEE QANKIWNHTF DEWQLQKDVY SYECYIALLA HQQRIDEAEK LFQEMI LKK DLSPSRRTYH CMIFMHLSSG EEGGTARALR YLEAMERAGI QVRPSLLKKI VRVNNAAGYK RDMKRRARRI MQAREEY LA RKEEGVSFGE GGKEGASNQR ADVDAEGNSI LEPLAVSPTS TLAWWEKWKR ETVSKHELFT EEGADGTPKG ETFEEKNE A LRMMGITSSF QTKDLVPQPD RQKLLPLIRR EEGEIAGSLW AMDGGELSYP KDGGGPQGWG VRLWRERQLV KREYQKVLD GYRPVPQLST LGNSVRTAGD QLDIERSGAQ TPGELSDYRN FPDNRFDGGQ LKPESEAAPA VPFSAELVWQ GEANDKLSPY KSDEEIALE NDNTFFSSLS KETEGKLSIA VKAMQNKEEN SVDVRGKGVT RRSKFDYLEK WRDMYRHGTL EVPEGPTLNF G RTPDDHKE TMAALVRGWY QRNRKEPASE EELKRWRVDE QRSSETSASR AALKKRKQVR SRHRNK

Macromolecule #51: mt-SAF3

• Macromolecule: Name: mt-SAF3 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 106.621727 KDa

Sequence

String:

MLRGWHPNSS AMQVGMRHIT IGGRHSRGGF RQPLGKHPQV KQGTVEGVPR RIPGTTKVTY TNKKGRTFSF SVPVSELTHP QVTLESAAG TWREMDTSFC ELGDIEDDMP SPVDECLRGG SSLDKRLIQE VRERFVSFCR EYVLMDTSGM KSTILSTELN A GPDYEHYD RRLRRKRHWL AIRHRFEDVR YVIWPDVVEE TARGDSAQAD VSLTNPSLTA GEMLEALLWL DAASTFCVRK VH PSDLGDK SEFLPLDLQR EVEVVACHAR RDLDFFDPSA TSLEQFTACA ALCVNHRVPF SLFFPAQDVC GDASVSTGQC IVA NAPSPH TALGAVRIMA LISEGSGSDI GKTIMFSDAF GAVTRFGILR GLSRVMSVEA FGCKDALENV NESELCIILH FCAE VREQN AAFFRRYEAS EEDSDPQQVS FLAKYQQLSQ IALARCKRLL YHPDSPRAQV MSEDGYIPLV ELQRHAEGTN KAALI HYNL GIRSAQGMRR VALGAQSSAR LAELVSRLEE ASARVSGNTL VNDLVHHLSH KAAAGKMSLT LREVNTLLPL LSRMRR ESP NGALDARFDR VFNAIDTAIG AAMRHNCTLD ELLDLAEGLA ACEMVPSALK QVEMVLIRSV MMHECSPMHL RRMLQAM FT LMRTSVPQVL LQSVASRVAD YIKEASHMDS SSSNGGGDEK VKNHEECEQL LELLVVLGKC GYGALPGLVT IYWEAQLI D SMQLNPRLRC SYASLLASAA FALKKHDKRA WEGLADESHR LFMEYTRCNK ENDIGRFAEC VTGLAVLTQI KDNTNSSDV AFLKEYLSAT SLELKSCEVI RVQELTDLLG RTLEWSEALG VVAPDVVIQL EKALFVMLEN VSHTAPGVGI PDELVTAACC LVDMSSASL ELRKAAAGVV GGAIVHAEEA LETLRSGAPT QVRPGHSFDV AALASAEREN VYKNSILQYC AALQRSGMST H VEELWS

Macromolecule #52: mt-SAF5

• Macromolecule: Name: mt-SAF5 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 86.949414 KDa

Sequence

String:

MRHTIPFFRR SAFVPAPGSS LLNPRSQRAK VRRMVAAQKA QGENFERQAL YAELGGSPSA RAPRSKGERS KEATRRVGCE VAERAKHMT DAEWEGVPVD EKHAFAKYMH KVLQEHPTET TEQQRRRYFE TTMADVFELD PRKTVRDEYE RVKLGLPVHL K NPQYSLGV SQAVYDAADA SLFDPENVHR LENAMTHVKQ VFADYVHKKR EGVSTEAERR MLANLTAELN LETQKHLANM FK YAEMRLR QVKLEERHHQ LAEIERLRRM AQQRGGVKGR KGGSRKMSRM ERLKRVINRA VGLDIAVAET VLTEMQAQEE FLQ FCEVFA RLTLGSGFKH TGKDENLSAY IESLRKLYSM DAATLSTLDV VQYYSSKEGA HPVDWAKRWY ERALLLPLQS TPEY QKLLQ IQQRDESTVK HIKETAGTGH AFACEAEAEV ARIKTQKVVN LVEKMFMDPK DKRLESLHEK RLRYLAHMQM ERQIR CVRE NAKLFDGVEN MPEAAQCREL YEKIMEKKTA QCNMTSPPEG EGSAIQSAKT EGDHCEVGPG MFNVYDDAEA SSLFEK IRE ITLRVIRDRR VQSAAATKAR MLNRIIRSLK GGERSIAEEL RALHQQRKEK MTMRILGIIE NDVKTEMEWL QNMEEAE RP PLLPIPENMS YVSAADVQAW RELREDDERK AANPFERRRR TFQPELLGQA WSVPNKPLLF WGTGVSAVQQ ALRHVAED A ERKRQGLLLA PPYPCAENPW GWRLAKDILD DNN

Macromolecule #53: DUF4460 domain-containing protein

• Macromolecule: Name: DUF4460 domain-containing protein / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 76.781398 KDa

Sequence

String:

MYKKALHSFF LKVHPDFFHH NRSQQTVNES SVARLNELLS WAKAFKSGHL QPPPSSSFTL TFYRKPDDNM GNGETRREGS GSPVSSLVG RSMGDGSLSP TIIQSTFELP SNFAPSDNHR GTVERAVNKF LRDLLRRAAC IDSVTESISE AEDATAARAE A KPLRRRPR GSQHRGAPTG PKSLLDEAVE SMTVQWSLTP APTLQELIEA DQILFSRDLS PLQSAAALST LQRHLGELNY SA WESMPVI VSNQFSIGDL TGTITIPWDF TPEQFHSFMA HNEKGVARCR EVAIQYASTI EQLIAELCTA LELDDILVSC SHQ DALRLM ELLHRNRELL IQYGLSKLTL EVGNRHATRA NGVVIINCSL TSEQLRPWLK AISPKLPLQQ RLYELSKQML ESTL WHLKE FRTMVEPGGV DAFSNDCTYA ERLQWSKELF RIGPSLAPWD WSEMTFVLSP DVDIDWANGL LALPYNFDGD ALVRY VEEV QQEAKSRKRE ELLAASAMQR EEEERRRKQQ HDEELMVECQ EKGERSGDDS PTVAERMRHL YRQTNPHMDE YLASSN SRV DTLPVERPLS HAVTFNSDAE AEDQLKWEGF YAEPYVDQAP TSDIDDMAHT FMLTNRWHRE EAAKKMLDQL RGTYGKK SR RFEYQKMGDV LEINNAKVQP KGFPTLTRGI KPGC

Macromolecule #54: mt-SAF7

• Macromolecule: Name: mt-SAF7 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 76.675156 KDa

Sequence

String:

MLSHAVPRLR LAAAAVKAAE WYAAENRRKQ KGGDVIREKV DCTAPLWFDD TEQNLLAREQ HVRSPAYREL HRPALTNAAM GLYGGEPGF EKAHRVWVDP DRPHIKHIYN QTALARNLRY ARYGYFKRDM HLLDVDKLVR HARLLPTPGR LLTDFLYQRV P LPDKSCAA LIRYQRQQIE MLEVWGRHAS FQCAVEMFER MIVTNIPPVE VGVETHGEMV LCAAACGKWE EGWNVYANRA RE LEKESPE SFILNTFFFD ALLTLCVAAG RVSEGIDTLE EVIKRNLRPR GTMLNKAMIL YSILGEQMSK HEASRATSEG ERS YLCEPE EVEKMGLEVW SLFDFYQLPR TTASIEAYMR MCCAFNKPTL VLKAQGFADA SDIRLSIECF HWLVYAIRGV AGFG DYVMD VLSQLRPRGL TPDFVLFTLS FMYCALQRDG ELALAIFDQH FVHQNMNPTP EMVLLFIQAC SNCEEPTAVM LERSE TLIK RLEAVGSSVD LISPIYDQFL ELCAHLGAVA SGFSALKRIV GFGKPLTTRM INSLLLANSN AISSNGSLSM TEELVG FFT LLKIRPNADT EICVNLCRDA FGESPVVNDF IKVIGESLQG DSEKGEAPQY DEDIPVIQVP PHELRQLRTE WKLSPRD IV LRRFGQHTKP PGKAALDVGS MRGSVIPFGR SPGEQLV

Macromolecule #55: mt-SAF9

• Macromolecule: Name: mt-SAF9 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 69.65268 KDa

Sequence

String:

MTGPTRALFL SSGINLGRLR LAEQFSSMNG WQSKEDPAFD AYVKERRRKE NYEAFDQRVE RGYAAAAKLH KAEIQNAVKR RLKSSGAKF TAETLREMSS AVTERLAWLR DVWAQIDADY RSGDSARQET AAQEISAALR GEPNDYMRWV YETKRELRFA G PVGRRAIQ EELQAAELPE VLDEEVNRYH DLKLNMMEIE REVKAKYGVA GQQHWAELQA AKDEEYIQKL DEAAEVYKQL LD QSARLDE SRRSELQRSY VERVHQAQVR FKAAMELEGQ REQLIEAHQA MKEERMRTER EKRRQLLREA AELRAQGKKS ADV LTALKE RQLDANAKRQ AEYELKECED ILKRKSEMLD MIAHFKHDVE EREGREMLQR QKSDERQVNV FGFYEEVGVE DGLS ISSEG TTSQGGSSGL GTVSTSTSCA KSADSNSSAQ PSQKLRKEEL WKVINADTYE DPFRTVHQAR LDAVKTYDPA YARTF PLNL VLGRKYSRQG AGEMAAGNET DKQILQKGNN ILYSFQWGLN NGTVHDLDAD GGTDYFMDGA FHVRDKETGD IDWRYE KKR GGPVFRGPKF YRLGAQREAA DPGERAMDPT PYTSTPREHK WRSS

Macromolecule #56: mt-SAF21

• Macromolecule: Name: mt-SAF21 / type: protein_or_peptide / ID: 56 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 39.638848 KDa

Sequence

String:

MSAIKRSLNV GVVGMGNMGV PIARNLGFKA RSAMYLQIHS RALSKAKRVC DDLSVDGATC AMRIHDRYST MTKWCDVIVL ALADVKASR HALLEDNESL IMNARKGQII VDHTTVDAET SRECAHEAAR RGAYFLDAPM SGSPRACFNG QLLLMVGGDA E TFQKMLPI FHMYADNVFH MGESGSGTAA KMISQALVAS HNAAAAEALS MAHALGIEDQ SKLVQVLDAS WGSSTMLRRN AP LLQDLIR NPDKTPPTSA VSVDRLLSDL AHLDASLPKR GGEDEPFPVF DAALRSLAAA SDAGMGDRDL ASVVHYIEAG EAE LRNRTH VPL(UNK)GEHLTG RTAATAANSS VNETPSTVNG SSEVPNEFGV EDFY

Macromolecule #57: mt-SAF22

• Macromolecule: Name: mt-SAF22 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 39.132621 KDa

Sequence

String:

MTDPVRRDPT EFLRVLRRMS RTTSWQKTML FASKGRMVGY RLTREHYNTV LFSQSLWGRA LEIVRVVRAM QEDKVQPNGA TYYYIVNGM GNADHGWNYD FRINRRLEKI QHWRVALEAL EACEANGFDS TDTMHNSALI TLVIPGFNRW QQASLLLQRM L REDRRMHP TMVKFYHDCL VRNNRPREAS SLMRLAAERG VHGYEDKWEA DVYKGRPLDS EVMNESEGNG LRRQASSLAF AS LMLRGDQ RPLPENLQAL LEEETTRNIE AERSVPVPFS AGLHATEINS VFRPRVYRQL WYKWQHIANR YRPTAALKRR QLA PRDSPT GIPGFYRI

Macromolecule #58: mt-SAF23

• Macromolecule: Name: mt-SAF23 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 38.240164 KDa

Sequence

String:

MVAKLQHNSA PTTLNFYEKS FQQLSDVQQR QTGLLIGAAV GDAAARALDG YTAEEVAAVA AESGSLQDED EDPVVFASVT PREHKSGLL RHHSYTFYLF SQLLRVMATS RGDFPVQYVK NEWVATARAH PDCFVREHAS LLHVLCITMQ LPVIYPWADD S TLREYASG FLEFLTETPA EQAVASREDV YAYTNSVLGV ALRCLQSNPD PYRNAAFMAA PGTAHVFPDD LALYCPPALV GS SHSRTEE LSETDSETVP LFPARLLESD VRVVRECLVV ARGAASFAEG IKAAIHLGGP VCQRSLIVGA LLGARMGVRR IPI SWLSAT YDHVPLVTLA LQVAQWSWNP PHH

Macromolecule #59: LMWPc domain-containing protein

• Macromolecule: Name: LMWPc domain-containing protein / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 30.538557 KDa

Sequence

String:

MSTSSRGILI AGLTNRARTQ MAEGILRRLT AGTIFIKSGG VHHESTIHPL AVKVMKDIGI DISKQSVTSL EAARRQQNTY DVYISIDCS YERRTLDKSQ PPRAEVRRRE SFTVEYGDDG SGPRGGDDPV RYDPLLVPRT PSHWSVGVDA TDVRRRWQIW S PRDPSIFH ETSTRKFQDH LYEGEPLFMQ LRQCPWRTQA KVSERWEMDE VAIRYPVERH SAHELRFVQA REQLLQRSFD LL TKLEKHY GERLLLDRAL LEEFIS

Macromolecule #60: mt-SAF27

• Macromolecule: Name: mt-SAF27 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 26.936748 KDa

Sequence

String:

MHPRLSGQGV FPRYGGGWMH QFGHVYALQQ ELLNAEANNW FRAMELWHTA RHEGVAMNVS HYTNILRQCV PPTAWEASLM VLKQMRREN IRPDVVGVGC ALAACADANR SEEVEKVFSC FSGKLKLDSI CYLALIKAKM SRGGYAEALA VGREQDADGV P FLPHTYTH LLEAAEVADD GEMALELARR MSSEQWPVSD RGREALKKLS TRHHWEEVAE YKQFVAVEDH KLSLPPTLPR G

Macromolecule #61: mt-SAF29

• Macromolecule: Name: mt-SAF29 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 20.048514 KDa

Sequence

String:

MRRKTTLNIG QVICFSSWND GSEGYEWKSR ALSEKRSLAL EFLGNVNKRV SIHDAIRLKA DINKKAISNV SCPSFFSGIE GADEDEDQS DMSLCSLLGV LEGEIETDCI THLSPSDASL LKEEFLCDYD PSDTKRMAKW VNLRSETSDY QSYGAIPEGE R SLWSAWYL RNIKAGKKPI

Macromolecule #62: mt-SAF31

• Macromolecule: Name: mt-SAF31 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 17.673535 KDa

Sequence

String:

MNCSSTLACH AVVSAPSTAS LITSCWTPQQ HCYRQLMKSL RAAYFHDRSK LFWSRHRVLV EFYKYSEEAN EEAVKQLVAI GLEVAAFID HHMRTDVERI VKHNETMMAL PVAQAKKFRS DYLLAEKQHD SWCKQKIKNI MKRRPPPPYP FF

Macromolecule #63: Acyl carrier protein

• Macromolecule: Name: Acyl carrier protein / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 16.550807 KDa

Sequence

String:

MQRSQLARKF ATQVGAYSLL RGRCHAVSTW KVMATQAVTI PMYLPVRFHS EGHAAGEEAG RTGQYLLNKD DVLTRVLEVV KNFEKVDAS KVTPQSHFVN DLGLNSLDVV EVVFAIEQEF ILDIPDHDAE KIQSITDAVE YISQNPMAK

Macromolecule #64: DUF4379 domain-containing protein

• Macromolecule: Name: DUF4379 domain-containing protein / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 16.291121 KDa

Sequence

String:

MRHFGPSIAH FPLSANQLRF KASHIRIANR KRVEMFVAKR FHLPTRLATA APDIAAEWHD ELNPMHMYPA IIGIGHVQPV WWKCAICSH SYQMSVEKRV VRGGGCPQCV VNGKRAVADG ALEGEMDSQL RPKRPVMFNM RTKY

Macromolecule #65: DNA photolyase, putative

• Macromolecule: Name: DNA photolyase, putative / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO / EC number: deoxyribodipyrimidine photo-lyase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 95.285312 KDa

Sequence

String:

MKRIFRSSVR VGAALPLGAV VPAIFYCNGI RIMGRQYTHD TYRPFGDGYK EVRGTAPTDE CNLLFASLND ESLPLCEDSG NVVNGQLVR ASALVDSDAL ADSQELVDVA IEEDRREEED NSVLLVP(UNK)HE EQNSVTKKQN EGEDVTTRCD HLFSVVG CP TTVSTATSGI PNTPSRNYLS EQDALYLSPT TPEEARVPSA LCPQLSACVM VVFAANDMRV HDNYALALAA TRAEAAGG L PVIAVTVVDY RMFAQPSAVG GFFRQSPMRA RFFLETLAAL RYKLERELRI PLLVRCGRPE DHIPRLVVEC GAIDVFLTT QYAPHEKEVH NDIVEAINQR RWVSRDPTAG YKAIEHDATV VHPFGATSAS TKVGVKESRL TAAPVAHSVW QTTLVHIDDL PVPVASMVE GERWYHDDVT TANIRPTAAF DRCINRLSDL PARGDLLPTT GELSGLEPPP LYRGRAPTLV ELGYGSPEAF I EEEVIATQ SSHPPGEDAA IERVLDWLMD GGMTSMLRYG RDRRTNTKMY SQRLSRLSPY LSCGALSPRR FYEELRRFTS EH LRDNFVQ QQYREALLRL SRRDYWHWMG LRYGPLLFYE YGPRPEHTDD IADWRHDPKI VQKWCAGLTG IPFADAAMRE LTL TGFVAD EGRQGLIWLL SRGYGQDWRL GAEWLERCSI DYDPFVCYGN CAYYSELVRD DFGETVRNIH WLAHHHDQTG IYVK KWLPQ LSKIPPVYIH RPHVLTERMQ AMHGVQLGKN YPYPLKLWDG AQYTLSSEHL TTYFSEGSQW HRKAKNGAGG SGYAE ALRH GTVILEPHQL QSSAFGELEH GVMHTVPVVK DDTAGRGQHN RSLTASDE

Macromolecule #66: Acyl transferase-like protein, putative

• Macromolecule: Name: Acyl transferase-like protein, putative / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 60.722109 KDa

Sequence

String:

MSKSLQRALV FSGQGAHRRG MATEYLRIPA VACLWERMKG SMEQKYGISL QDIITENPTR LYVYNDAYDV AAICERSCQD GGSVVESTP KQKLISHPQG VLSLTSLTQP CMLAAHMVSL EYLKETRGYS VESSEVIAGH SLGEFSALCA LGVLSPEVAI D LVYRRGAL MEDAVKHSSQ SNEGHLMFAC NPQRAKLCVE DPDLAVDQLH IFVELIARNL STTASFIEVV NYNISYEQYV VV GDPIALS ALGKCLDPQF RATSCGSSAT LDNIVRTAVS SVLQDKQEGI TMNPNTGPAP DFVTSCARKY GIRSTFRRFL RGP DDGYTP SLEELTHLTL QEDGRSGLKK KSWFIPLPLS IPFHSSMLRR AMDLFLPVVR DALPDEETMR SFFCIPQCGG SD (UNK)GPSSRC ASEPKKPVWL TNLTGTAFRP FDVDFQRDAL DAMQSMNVGE IRHNGRYHTN LVELAFKNGM DTSSVRDMC AAVLAAQLAH PVQWIDVMDS AVFQHGVREA HEISPVRTVA DMFKRTVFRG KTSDASVGEA ALDIVTRCLP SEERFL

Macromolecule #67: mt-SAF37

• Macromolecule: Name: mt-SAF37 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
• Molecular weight: Theoretical: 37.00909 KDa

Sequence

String:

MWQRLRFDRL SSSVRRTNLN PLKPCAALTE QRAELRNLHQ YPTARHKSLV KDRLRFARNW WLTGGNNYEL VHEVGHEREA TECFAEYAQ DSSRDVYLMS TNRLSDLPPG DRLKAIVGLM RSRWEVKDAN RGYDKAKLLL QALECFSEMK ASGQIGDFNS L PEPDQDTF LQYVEGCSRF AQACSHSHPD AVRVLLRAAQ ICEEMRCVEK RDEMIQVTEA AANRMDRAYA FSRPHDTLRA AP PSLHENE DCVRLKNTEE LRRRFGNTAP HVLEKPKRVD CLRIHRNRPL LLHPMKDNNK LLELSKLPAR PEFDSWTSHQ T

Macromolecule #68: mt-SAF38

• Macromolecule: Name: mt-SAF38 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 69.516695 KDa

Sequence

String:

MRGSLPLLFN PVLPPSTARL RLLTYPMALA QPHATVPLIQ PTIDGTHDGR NGATVSLRTQ ARMHGTADGT MATAGDSSQN NSVMDSPRW LRNPDELCVA ALRRSRDVNK INSYVATYKF DDPQWAPLLL PEVTLRPQVN STGDKPNGGN EAAADVVSVG P SVSATPES TPPPPPSSSS SSPYSCPADC VSISHNKMIM LECMSRHVNF SLRHIVQKGH GIYLIYHAQH SILQPKGLVE QS FVTCSFG IRGERLRTDI VHVGPIDAAD VMELQPSEGH DHPRCCFNLY QKSDVRRGVI AVSQVEGYGT WFQRKPMLWQ RSR RIGALQ SQLGAFAYDL VDPHEVGKWR DCEVSLLAPH MRFFRNGLNG AEAVGIIASS QVAQQRRLYL GEFEAPAITA LDAV QQLAH ASALRCKLVT PVVDPNGVGG TGSGSLGDEN MDKHIDMETL LPLSWATRTP PPYVPLEADL PFKLQMSRPT VFAES HQQN QAYPTGGTVG SPFVRGAPMM MFEYNMHQGV DHYVYDDAPS ARPMKWWSQK SNMPYSGYMY FARSGLVDRF TPSEDI PNP LEPTSKRKPL HAVVPPTKVV QERLRKYRRK QQEGHKQRRR ASSGSGVSNE PDAVNRQESV SRGTCE

Macromolecule #69: Translation initiation factor IF-2, putative

• Macromolecule: Name: Translation initiation factor IF-2, putative / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 87.113992 KDa

Sequence

String:

MQSGLCHCVR RVAALGSVAN LHHFTNLPSG FSVWTSRRWQ SQSSRGQPGL QWQEGVVDPR YSSSGNMRRF NPDTVAHYVK ATIQNDRRE MGLGEVYDWH EFAKDAVYIP TRSGPLWVGS DDPRCAKFMR RRSKMQKTPQ QKARPKPGPD PAKALEDHPL R EYFTTATN LRDPLSTANN LHRAGLIREY DIKYTAAKVR YVPRPPVVSI MGHVDHGKTT LLDYLRKTNV ASQEAGGITQ NV GAFQVKT LGDTLVTFID TPGHAAFTTM REVGATANDL IVLVVSAVDG VQPQTKEVIE LAHKSGIPFV VACTKIDRQP RVE NVKQQL RDCNVELEED GGDTQFVPVC ARDGRGVPEL LEAIALQAEL CEISTPKPSR CEVTVIETNG VNTTTEVAGI VRCG KVRAG QVLVAGMTYA VVRKVLDEHG QIIQEAGPSQ PVVLHGFRVH PKPGSILLQV SSESHAQKFY HFMKEVYQAE GRRED YLQL LNQEQHGMLY ARKPDNNLVR TYSTQAFVLS CKAATFGMLQ ALMKSIYEIP RLEGISTEIK VTEVGGLRDY DVALIG SSG QPGCILLYGG CKDTNTLDVP NHVTVIRFNV LYHGLEALKE TLVGALPKIT KIRVTAEAEC LQVFRASQAG KSGNAGG MR VTKGTIIAAH LTFRVCRKAP LVKGTAETDG VSDNEGDGDE DMLGHRVVVY EGQIKELRRF KELVPSVELG LECGVIMQ D EFQFRTGDIL QQYETYEEPR DVAEEYQKAE LREKIMRDTA AAEALLAEST GGEDKPTEES EVKTTI

Macromolecule #70: mt-SAF39

• Macromolecule: Name: mt-SAF39 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
• Molecular weight: Theoretical: 89.639992 KDa

Sequence

String:

MRRSGRGSAV RWSSLCKCQC CLYRTPLGGT YFEQALPRSL GARQGKGVLS TVNTALSRKA LKRRQSLPRK KLNVPLTAEG LKERLKQLS AEERELSIKN NTEECDEPSP NEFTTTHEAR VALARVLHHG ENAGERKEVA MRIPSFCRSP AVSETQSIVV D DKEGDITN AAVHVGCSVL GSDLDHLERD MIRDYHQRGK KLPTFDNIYR TLGCGRKGTS VSDTEPEDEN SSGAIQSECG LG DAGRRGT VVVAPSHLHH STPPTKGRSG EEEEEGGCFD TNTLPADANP HFPPGACDNE VLAPLSGGCA ASEQTEITDT ASF IPSNSR LSTAVYDAYR QRPADDRLVV LRGTDFWDNE ENRARLQELT DYAEEDFARE MLMEGAMDTS EVGYSTNKVR KETL LYFQA HPINEMIQEP FARVRSILPS DGGPEVHFPA DDPDTDVDIP TAQARTMARE LGLDLIRVGT LYTPINDRRV VAVCT IADH REHMRDMIRF KIKKLGVQRP PTKEGIEVPF RGGTHPHAVR FKSIGIAKHL LLGHVVRINL TDFGTVREGF PVFGSI LDE VARQALQLHA YHTAGVVRAN YNEVYCYLYP STGRSPKSTV LHPTQEQLAT VRDRCLLERE REVYFDGLYD KKTPRER LT YMRKLQDGTA WADRDDGLSL QRQRDMKVML GYLPKGNHEL YAARGDVNVP APFRASHPTS VDRWTHPQES NLEQAARG S AVLAKRLSMT VSEMHDRQET AENPATLDRF YYRIQGPALE AGELKEALGL KGNRKRLPRR APGWATLGME KVSPQEPGH AAK

Macromolecule #71: Unk8

• Macromolecule: Name: Unk8 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 2.571161 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #72: UnkC

• Macromolecule: Name: UnkC / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 783.958 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #73: UnkD

• Macromolecule: Name: UnkD / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 1.22447 KDa

Sequence

String:

AAAAFVLFMA AAA

Macromolecule #74: UnkF

• Macromolecule: Name: UnkF / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 3.08179 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #75: UnkG

• Macromolecule: Name: UnkG / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 1.459621 KDa

Sequence

String:

AAAAAAETDW KVIAA

Macromolecule #76: UnkI

• Macromolecule: Name: UnkI / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 528.644 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #77: UnkK

• Macromolecule: Name: UnkK / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 2.060531 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #78: Unk

• Macromolecule: Name: Unk / type: protein_or_peptide / ID: 78 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 698.854 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #79: UnkP

• Macromolecule: Name: UnkP / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 2.74137 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #80: Unka

• Macromolecule: Name: Unka / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 869.063 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #81: Unkg

• Macromolecule: Name: Unkg / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 23.081336 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #82: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 82 / Number of copies: 7 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #83: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 83 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Macromolecule #84: PHOSPHATE ION

• Macromolecule: Name: PHOSPHATE ION / type: ligand / ID: 84 / Number of copies: 2 / Formula: PO4
• Molecular weight: Theoretical: 94.971 Da

Chemical component information

ChemComp-PO4:
PHOSPHATE ION / Phosphate

Macromolecule #85: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYL...

• Macromolecule: Name: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE; type: ligand / ID: 85 / Number of copies: 1 / Formula: PM8
• Molecular weight: Theoretical: 496.598 Da

Chemical component information

ChemComp-PM8:
S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE

Macromolecule #86: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 86 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #87: DIHYDROFLAVINE-ADENINE DINUCLEOTIDE

• Macromolecule: Name: DIHYDROFLAVINE-ADENINE DINUCLEOTIDE / type: ligand / ID: 87 / Number of copies: 1 / Formula: FDA
• Molecular weight: Theoretical: 787.566 Da

Chemical component information

ChemComp-FDA:
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE

Macromolecule #88: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 88 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

Macromolecule #89: water

• Macromolecule: Name: water / type: ligand / ID: 89 / Number of copies: 5 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 98 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 40.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: Gctf
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 38531