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TitlePspA adopts an ESCRT-III-like fold and remodels bacterial membranes.
Journal, issue, pagesCell, Vol. 184, Issue 14, Page 3674-3688.e18, Year 2021
Publish dateJul 8, 2021
AuthorsBenedikt Junglas / Stefan T Huber / Thomas Heidler / Lukas Schlösser / Daniel Mann / Raoul Hennig / Mairi Clarke / Nadja Hellmann / Dirk Schneider / Carsten Sachse /
PubMed AbstractPspA is the main effector of the phage shock protein (Psp) system and preserves the bacterial inner membrane integrity and function. Here, we present the 3.6 Å resolution cryoelectron microscopy ...PspA is the main effector of the phage shock protein (Psp) system and preserves the bacterial inner membrane integrity and function. Here, we present the 3.6 Å resolution cryoelectron microscopy (cryo-EM) structure of PspA assembled in helical rods. PspA monomers adopt a canonical ESCRT-III fold in an extended open conformation. PspA rods are capable of enclosing lipids and generating positive membrane curvature. Using cryo-EM, we visualized how PspA remodels membrane vesicles into μm-sized structures and how it mediates the formation of internalized vesicular structures. Hotspots of these activities are zones derived from PspA assemblies, serving as lipid transfer platforms and linking previously separated lipid structures. These membrane fusion and fission activities are in line with the described functional properties of bacterial PspA/IM30/LiaH proteins. Our structural and functional analyses reveal that bacterial PspA belongs to the evolutionary ancestry of ESCRT-III proteins involved in membrane remodeling.
External linksCell / PubMed:34166616
MethodsEM (helical sym.)
Resolution3.6 - 7.15 Å
Structure data

11698

7abk

EMDB-11698, PDB-7abk:
Helical structure of PspA
Method: EM (helical sym.) / Resolution: 3.6 Å

EMDB-12733:
PspA helical structure in presence of lipids
Method: EM (helical sym.) / Resolution: 7.15 Å

Source
  • synechocystis sp. (strain pcc 6803 / kazusa) (bacteria)
  • Synechocystis sp. PCC 6803 (bacteria)
  • Synechocystis sp. PCC 6803 substr. Kazusa (bacteria)
KeywordsLIPID BINDING PROTEIN / PspA / IM30 / Vipp1 / ESCRT-III / helical reconstruction / Cryo-EM / membrane remodeling

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