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Structure paper

TitleStructure of the mature kinetoplastids mitoribosome and insights into its large subunit biogenesis.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 117, Issue 47, Page 29851-29861, Year 2020
Publish dateNov 24, 2020
AuthorsHeddy Soufari / Florent Waltz / Camila Parrot / Stéphanie Durrieu-Gaillard / Anthony Bochler / Lauriane Kuhn / Marie Sissler / Yaser Hashem /
PubMed AbstractKinetoplastids are unicellular eukaryotic parasites responsible for such human pathologies as Chagas disease, sleeping sickness, and leishmaniasis. They have a single large mitochondrion, essential ...Kinetoplastids are unicellular eukaryotic parasites responsible for such human pathologies as Chagas disease, sleeping sickness, and leishmaniasis. They have a single large mitochondrion, essential for the parasite survival. In kinetoplastid mitochondria, most of the molecular machineries and gene expression processes have significantly diverged and specialized, with an extreme example being their mitochondrial ribosomes. These large complexes are in charge of translating the few essential mRNAs encoded by mitochondrial genomes. Structural studies performed in already highlighted the numerous peculiarities of these mitoribosomes and the maturation of their small subunit. However, several important aspects mainly related to the large subunit (LSU) remain elusive, such as the structure and maturation of its ribosomal RNA. Here we present a cryo-electron microscopy study of the protozoans and mitoribosomes. For both species, we obtained the structure of their mature mitoribosomes, complete rRNA of the LSU, as well as previously unidentified ribosomal proteins. In addition, we introduce the structure of an LSU assembly intermediate in the presence of 16 identified maturation factors. These maturation factors act on both the intersubunit and the solvent sides of the LSU, where they refold and chemically modify the rRNA and prevent early translation before full maturation of the LSU.
External linksProc Natl Acad Sci U S A / PubMed:33168716 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 3.9 Å
Structure data

11796

7aih

EMDB-11796, PDB-7aih:
The Large subunit of the Kinetoplastid mitochondrial ribosome
Method: EM (single particle) / Resolution: 3.6 Å

11821

7am2

EMDB-11821, PDB-7am2:
Intermediate assembly of the Large subunit from Leishmania major mitochondrial ribosome
Method: EM (single particle) / Resolution: 3.4 Å

11829

7ane

EMDB-11829, PDB-7ane:
Leishmania Major mitochondrial ribosome
Method: EM (single particle) / Resolution: 3.9 Å

11846

7aor

EMDB-11846, PDB-7aor:
mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / Nicotinamide adenine dinucleotide

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM / Uridine triphosphate

Source
  • leishmania tarentolae (eukaryote)
  • leishmania major (eukaryote)
  • trypanosoma cruzi (strain cl brener) (eukaryote)
  • trypanosoma cruzi (eukaryote)
KeywordsRIBOSOME / Mitochondria / Kinetoplastid

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