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TitleTrans-synaptic assemblies link synaptic vesicles and neuroreceptors.
Journal, issue, pagesSci Adv, Vol. 7, Issue 10, Year 2021
Publish dateMar 5, 2021
AuthorsAntonio Martinez-Sanchez / Ulrike Laugks / Zdravko Kochovski / Christos Papantoniou / Luca Zinzula / Wolfgang Baumeister / Vladan Lučić /
PubMed AbstractSynaptic transmission is characterized by fast, tightly coupled processes and complex signaling pathways that require a precise protein organization, such as the previously reported nanodomain ...Synaptic transmission is characterized by fast, tightly coupled processes and complex signaling pathways that require a precise protein organization, such as the previously reported nanodomain colocalization of pre- and postsynaptic proteins. Here, we used cryo-electron tomography to visualize synaptic complexes together with their native environment comprising interacting proteins and lipids on a 2- to 4-nm scale. Using template-free detection and classification, we showed that tripartite trans-synaptic assemblies (subcolumns) link synaptic vesicles to postsynaptic receptors and established that a particular displacement between directly interacting complexes characterizes subcolumns. Furthermore, we obtained de novo average structures of ionotropic glutamate receptors in their physiological composition, embedded in plasma membrane. These data support the hypothesis that synaptic function is carried by precisely organized trans-synaptic units. It provides a framework for further exploration of synaptic and other large molecular assemblies that link different cells or cellular regions and may require weak or transient interactions to exert their function.
External linksSci Adv / PubMed:33674312 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution29.184001 - 36.480001 Å
Structure data

EMDB-11404:
Native putative AMPA-type ionotropic glutamate receptor, de novo
Method: EM (subtomogram averaging) / Resolution: 33.673847 Å

EMDB-11405:
Native putative NMDA-type ionotropic glutamate receptor, de novo
Method: EM (subtomogram averaging) / Resolution: 33.673847 Å

EMDB-11406:
Native putative AMPAR-type glutamate receptor, de novo 3D classification
Method: EM (subtomogram averaging) / Resolution: 31.268572 Å

EMDB-11407:
Native putative NMDAR type glutaamate receptor, de novo 3D classification
Method: EM (subtomogram averaging) / Resolution: 36.480001 Å

EMDB-11408:
Native putative AMPAR-type glutamate receptor, de novo 3D classification C2
Method: EM (subtomogram averaging) / Resolution: 31.268572 Å

EMDB-11409:
Native putative NMDAR-type glutamate receptor, de novo 3D classification C2
Method: EM (tomography) / Resolution: 29.184001 Å

EMDB-11410:
Native putative AMPAR type glutamate receptor, model guided
Method: EM (tomography) / Resolution: 33.673847 Å

EMDB-11411:
Native putative NMDAR type glutamate receptor, model guided
Method: EM (subtomogram averaging) / Resolution: 33.673847 Å

Source
  • Rattus norvegicus (Norway rat)

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