Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of a mitochondrial ATP synthase with bound native cardiolipin.
Journal, issue, pages	Elife, Vol. 8, Year 2019
Publish date	Nov 18, 2019
Authors	Alexander Mühleip / Sarah E McComas / Alexey Amunts /
PubMed Abstract	The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum ...The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum Euglenozoa that also includes human parasites. It features 29 different subunits, 8 of which are newly identified. The membrane region was determined to 2.8 Å resolution, enabling the identification of 37 associated lipids, including 25 cardiolipins, which provides insight into protein-lipid interactions and their functional roles. The rotor-stator interface comprises four membrane-embedded horizontal helices, including a distinct subunit . The dimer interface is formed entirely by phylum-specific components, and a peripherally associated subcomplex contributes to the membrane curvature. The central and peripheral stalks directly interact with each other. Last, the ATPase inhibitory factor 1 (IF) binds in a mode that is different from human, but conserved in Trypanosomatids.
External links	Elife / PubMed:31738165 / PubMed Central
Methods	EM (single particle)
Resolution	2.8 - 4.32 Å
Structure data	10467 6tdu EMDB-10467, PDB-6tdu: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, full dimer, rotational states 1 Method: EM (single particle) / Resolution: 4.32 Å 10468 6tdv EMDB-10468, PDB-6tdv: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, membrane region Method: EM (single particle) / Resolution: 2.8 Å 10469 6tdw EMDB-10469, PDB-6tdw: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, peripheral stalk, rotational state 1 Method: EM (single particle) / Resolution: 3.8 Å 10470 6tdx EMDB-10470, PDB-6tdx: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, rotor, rotational state 1 Method: EM (single particle) / Resolution: 3.3 Å 10471 6tdy EMDB-10471, PDB-6tdy: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring in rotational state 1 Method: EM (single particle) / Resolution: 3.04 Å 10472 6tdz EMDB-10472, PDB-6tdz: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 2 Method: EM (single particle) / Resolution: 3.14 Å 10473 6te0 EMDB-10473, PDB-6te0: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 Method: EM (single particle) / Resolution: 3.92 Å EMDB-10474: Cryo-EM structure of the Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 with bound IF1 Method: EM (single particle) / Resolution: 4.1 Å EMDB-10475: Cryo-EM structure of the Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 without IF1 Method: EM (single particle) / Resolution: 4.2 Å
Chemicals	ChemComp-CDL: CARDIOLIPIN / phospholipidYM / Cardiolipin ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / detergentYM ChemComp-LPP: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / phospholipidYM ChemComp-TRT: FRAGMENT OF TRITON X-100 / detergentYM / Triton X-100 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM / Adenosine diphosphate
Source	euglena gracilis (euglena)
Keywords	MEMBRANE PROTEIN / mitochondria / ATP synthase