Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16.
Journal, issue, pages	Cell Host Microbe, Vol. 27, Issue 2, Page 249-261.e5, Year 2020
Publish date	Feb 12, 2020
Authors	Maozhou He / Longfa Xu / Qingbing Zheng / Rui Zhu / Zhichao Yin / Zhenghui Zha / Yu Lin / Lisheng Yang / Yang Huang / Xiangzhong Ye / Shuxuan Li / Wangheng Hou / Yangtao Wu / Jinle Han / Dongxiao Liu / Zekai Li / Zhenqin Chen / Hai Yu / Yuqiong Que / Yingbin Wang / Xiaodong Yan / Jun Zhang / Ying Gu / Z Hong Zhou / Tong Cheng / Shaowei Li / Ningshao Xia /
PubMed Abstract	Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific ...Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific neutralizing monoclonal antibodies (nAbs) with therapeutic potential: 18A7, 14B10, and NA9D7. We present atomic structures of these nAbs bound to all three viral particle forms-the mature virion, A-particle, and empty particle-and show that each Fab can simultaneously occupy the mature virion. Additionally, 14B10 or NA9D7 provide 100% protection against lethal CVA16 infection in a neonatal mouse model. 18A7 binds to a non-conserved epitope present in all three particles, whereas 14B10 and NA9D7 recognize broad protective epitopes but only bind the mature virion. NA9D7 targets an immunodominant site, which may overlap the receptor-binding site. These findings indicate that CVA16 vaccines should be based on mature virions and that these antibodies could be used to discriminate optimal virion-based immunogens.
External links	Cell Host Microbe / PubMed:32027857 / PubMed Central
Methods	EM (single particle)
Resolution	2.65 - 3.78 Å
Structure data	0887 6lha EMDB-0887, PDB-6lha: The cryo-EM structure of coxsackievirus A16 mature virion Method: EM (single particle) / Resolution: 3.56 Å 0888 6lhb EMDB-0888, PDB-6lhb: The cryo-EM structure of coxsackievirus A16 A-particle Method: EM (single particle) / Resolution: 3.33 Å 0889 6lhc EMDB-0889, PDB-6lhc: The cryo-EM structure of coxsackievirus A16 empty particle Method: EM (single particle) / Resolution: 3.43 Å 0890 6lhk EMDB-0890, PDB-6lhk: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab 18A7 Method: EM (single particle) / Resolution: 2.65 Å 0891 6lhl EMDB-0891, PDB-6lhl: The cryo-EM structure of coxsackievirus A16 A-particle in complex with Fab 18A7 Method: EM (single particle) / Resolution: 3.07 Å 0892 6lho EMDB-0892, PDB-6lho: The cryo-EM structure of coxsackievirus A16 empty particle in complex with Fab 18A7 Method: EM (single particle) / Resolution: 3.13 Å 0894 6lhp EMDB-0894, PDB-6lhp: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab 14B10 Method: EM (single particle) / Resolution: 3.3 Å 0895 6lhq EMDB-0895, PDB-6lhq: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab NA9D7 Method: EM (single particle) / Resolution: 3.06 Å 0897 6lht EMDB-0897, PDB-6lht: Localized reconstruction of coxsackievirus A16 mature virion in complex with Fab 18A7 Method: EM (single particle) / Resolution: 3.67 Å EMDB-0898: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fabs 18A7, 14B10 and NA9D7 Method: EM (single particle) / Resolution: 3.78 Å
Chemicals	ChemComp-SPH: SPHINGOSINE / Sphingosine
Source	coxsackievirus a16 mus musculus (house mouse)
Keywords	VIRUS / VIRAL PROTEIN / localized reconstruction