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TitleA complete data processing workflow for cryo-ET and subtomogram averaging.
Journal, issue, pagesNat Methods, Vol. 16, Issue 11, Page 1161-1168, Year 2019
Publish dateOct 14, 2019
AuthorsMuyuan Chen / James M Bell / Xiaodong Shi / Stella Y Sun / Zhao Wang / Steven J Ludtke /
PubMed AbstractElectron cryotomography is currently the only method capable of visualizing cells in three dimensions at nanometer resolutions. While modern instruments produce massive amounts of tomography data ...Electron cryotomography is currently the only method capable of visualizing cells in three dimensions at nanometer resolutions. While modern instruments produce massive amounts of tomography data containing extremely rich structural information, data processing is very labor intensive and the results are often limited by the skills of the personnel rather than the data. We present an integrated workflow that covers the entire tomography data processing pipeline, from automated tilt series alignment to subnanometer resolution subtomogram averaging. Resolution enhancement is made possible through the use of per-particle per-tilt contrast transfer function correction and alignment. The workflow greatly reduces human bias, increases throughput and more closely approaches data-limited resolution for subtomogram averaging in both purified macromolecules and cells.
External linksNat Methods / PubMed:31611690 / PubMed Central
MethodsEM (tomography)
Resolution8.4 - 15.0 Å
Structure data

EMDB-0529:
Purified ribosome - A complete data processing workflow for CryoET and subtomogram averaging
Method: EM (tomography) / Resolution: 8.4 Å

EMDB-0530:
AcrAB-TolC from cellular tomogram - a complete data processing workflow for CryoET and subtomogram averaging
Method: EM (tomography) / Resolution: 15.0 Å

Source
  • Oryctolagus cuniculus (rabbit)
  • Escherichia coli BL21(DE3) (bacteria)

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