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-Structure paper
タイトル | Mastigoneme structure reveals insights into the O-linked glycosylation code of native hydroxyproline-rich helices. |
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ジャーナル・号・ページ | Cell, Vol. 187, Issue 8, Page 1907-1921.e16, Year 2024 |
掲載日 | 2024年3月22日 |
著者 | Jin Dai / Meisheng Ma / Qingwei Niu / Robyn J Eisert / Xiangli Wang / Poulomi Das / Karl F Lechtreck / Susan K Dutcher / Rui Zhang / Alan Brown / |
PubMed 要旨 | Hydroxyproline-rich glycoproteins (HRGPs) are a ubiquitous class of protein in the extracellular matrices and cell walls of plants and algae, yet little is known of their native structures or ...Hydroxyproline-rich glycoproteins (HRGPs) are a ubiquitous class of protein in the extracellular matrices and cell walls of plants and algae, yet little is known of their native structures or interactions. Here, we used electron cryomicroscopy (cryo-EM) to determine the structure of the hydroxyproline-rich mastigoneme, an extracellular filament isolated from the cilia of the alga Chlamydomonas reinhardtii. The structure demonstrates that mastigonemes are formed from two HRGPs (a filament of MST1 wrapped around a single copy of MST3) that both have hyperglycosylated poly(hydroxyproline) helices. Within the helices, O-linked glycosylation of the hydroxyproline residues and O-galactosylation of interspersed serine residues create a carbohydrate casing. Analysis of the associated glycans reveals how the pattern of hydroxyproline repetition determines the type and extent of glycosylation. MST3 possesses a PKD2-like transmembrane domain that forms a heteromeric polycystin-like cation channel with PKD2 and SIP, explaining how mastigonemes are tethered to ciliary membranes. |
リンク | Cell / PubMed:38552624 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 Å |
構造データ | EMDB-43889: Chlamydomonas reinhardtii mastigoneme (constituent map 1) EMDB-43890: Chlamydomonas reinhardtii mastigoneme (constituent map 2) EMDB-43891: Chlamydomonas reinhardtii mastigoneme (constituent map 3) EMDB-43892: Composite cryo-EM map of the Chlamydomonas reinhardtii mastigoneme |
化合物 | PDB-1aio: ChemComp-GLA: |
由来 |
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キーワード | STRUCTURAL PROTEIN (タンパク質) / Mastigoneme / Glycosylated hydroxyproline |