EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of prefusion SIV envelope trimer.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 29, Issue 11, Page 1080-1091, Year 2022
掲載日	2022年11月7日
著者	Jason Gorman / Chunyan Wang / Rosemarie D Mason / Alexandra F Nazzari / Hugh C Welles / Tongqing Zhou / Julian W Bess / Tatsiana Bylund / Myungjin Lee / Yaroslav Tsybovsky / Raffaello Verardi / Shuishu Wang / Yongping Yang / Baoshan Zhang / Reda Rawi / Brandon F Keele / Jeffrey D Lifson / Jun Liu / Mario Roederer / Peter D Kwong /
PubMed 要旨	Simian immunodeficiency viruses (SIVs) are lentiviruses that naturally infect non-human primates of African origin and seeded cross-species transmissions of HIV-1 and HIV-2. Here we report prefusion ...Simian immunodeficiency viruses (SIVs) are lentiviruses that naturally infect non-human primates of African origin and seeded cross-species transmissions of HIV-1 and HIV-2. Here we report prefusion stabilization and cryo-EM structures of soluble envelope (Env) trimers from rhesus macaque SIV (SIV) in complex with neutralizing antibodies. These structures provide residue-level definition for SIV-specific disulfide-bonded variable loops (V1 and V2), which we used to delineate variable-loop coverage of the Env trimer. The defined variable loops enabled us to investigate assembled Env-glycan shields throughout SIV, which we found to comprise both N- and O-linked glycans, the latter emanating from V1 inserts, which bound the O-link-specific lectin jacalin. We also investigated in situ SIV-Env trimers on virions, determining cryo-electron tomography structures at subnanometer resolutions for an antibody-bound complex and a ligand-free state. Collectively, these structures define the prefusion-closed structure of the SIV-Env trimer and delineate variable-loop and glycan-shielding mechanisms of immune evasion conserved throughout SIV evolution.
リンク	Nat Struct Mol Biol / PubMed:36344847 / PubMed Central
手法	EM (サブトモグラム平均) / EM (単粒子)
解像度	4.12 - 10.0 Å
構造データ	EMDB-25062: In-situ structure of SIV trimer 手法: EM (サブトモグラム平均) / 解像度: 8.5 Å EMDB-25063: in situ SIVmac239-Env trimers on the surface of AT-2-inactivated virions 手法: EM (サブトモグラム平均) / 解像度: 7.0 Å EMDB-25064: in situ SIVmac239-Env trimers on the surface of AT-2-inactivated virions 手法: EM (サブトモグラム平均) / 解像度: 7.0 Å EMDB-25065: In-situ structure of SIVmac239-Env trimers with ITS90.03 associated 手法: EM (サブトモグラム平均) / 解像度: 10.0 Å EMDB-27631: SIV mac239 SOS-2P K169T Env trimer with bNAb PGT145 and jacalin 手法: EM (単粒子) / 解像度: 7.64 Å 27718 8dua EMDB-27718, PDB-8dua: SIV E660.CR54 SOS-2P Env Trimer with ITS92.02 手法: EM (単粒子) / 解像度: 4.32 Å 27735 8dvd EMDB-27735, PDB-8dvd: Cryo-EM structure of SIVmac239 SOS-2P Env trimer in complex with human bNAb PGT145 手法: EM (単粒子) / 解像度: 4.12 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-MAN: alpha-D-mannopyranose / α-D-マンノピラノ-ス / マンノース
由来	Simian-Human immunodeficiency virus (ヒト免疫不全ウイルス) Artocarpus heterophyllus (ジャックフルーツ) Influenza A virus (A型インフルエンザウイルス) simian immunodeficiency virus (サル免疫不全ウイルス) macaca mulatta (アカゲザル) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / SIV / ITS92 / HIV-1 / quaternary (第四紀) / HIV-2 (ヒト免疫不全ウイルス) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性) / CD4 / SOSIP / Vaccine (ワクチン) / IMMUNE SYSTEM (免疫系) / VIRAL PROTEIN (ウイルスタンパク質)