EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Role of aIF5B in archaeal translation initiation.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 50, Issue 11, Page 6532-6548, Year 2022
掲載日	2022年6月24日
著者	Ramy Kazan / Gabrielle Bourgeois / Christine Lazennec-Schurdevin / Eric Larquet / Yves Mechulam / Pierre-Damien Coureux / Emmanuelle Schmitt /
PubMed 要旨	In eukaryotes and in archaea late steps of translation initiation involve the two initiation factors e/aIF5B and e/aIF1A. In eukaryotes, the role of eIF5B in ribosomal subunit joining is established ...In eukaryotes and in archaea late steps of translation initiation involve the two initiation factors e/aIF5B and e/aIF1A. In eukaryotes, the role of eIF5B in ribosomal subunit joining is established and structural data showing eIF5B bound to the full ribosome were obtained. To achieve its function, eIF5B collaborates with eIF1A. However, structural data illustrating how these two factors interact on the small ribosomal subunit have long been awaited. The role of the archaeal counterparts, aIF5B and aIF1A, remains to be extensively addressed. Here, we study the late steps of Pyrococcus abyssi translation initiation. Using in vitro reconstituted initiation complexes and light scattering, we show that aIF5B bound to GTP accelerates subunit joining without the need for GTP hydrolysis. We report the crystallographic structures of aIF5B bound to GDP and GTP and analyze domain movements associated to these two nucleotide states. Finally, we present the cryo-EM structure of an initiation complex containing 30S bound to mRNA, Met-tRNAiMet, aIF5B and aIF1A at 2.7 Å resolution. Structural data shows how archaeal 5B and 1A factors cooperate to induce a conformation of the initiator tRNA favorable to subunit joining. Archaeal and eukaryotic features of late steps of translation initiation are discussed.
リンク	Nucleic Acids Res / PubMed:35694843 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.7 - 3.6 Å
構造データ	14579 7zag EMDB-14579, PDB-7zag: Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA,mRNA, aIF1A and the C-terminal domain of aIF5B. 手法: EM (単粒子) / 解像度: 2.77 Å 14580 7zah EMDB-14580, PDB-7zah: Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA, aIF1A and aIF5B 手法: EM (単粒子) / 解像度: 2.7 Å 14581 7zai EMDB-14581, PDB-7zai: Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA and aIF1A. 手法: EM (単粒子) / 解像度: 2.6 Å 14731 7zhg EMDB-14731, PDB-7zhg: High-resolution cryo-EM structure of Pyrococcus abyssi 30S ribosomal subunit bound to mRNA and initiator tRNA anticodon stem-loop 手法: EM (単粒子) / 解像度: 2.25 Å 14763 7zki EMDB-14763, PDB-7zki: Cryo-EM structure of aIF1A:aIF5B:Met-tRNAiMet complex from a Pyrococcus abyssi 30S initiation complex 手法: EM (単粒子) / 解像度: 3.6 Å PDB-7yyp: Structure of aIF5B from Pyrococcus abyssi complexed with GDP 手法: X-RAY DIFFRACTION / 解像度: 2.9 Å PDB-7yzn: Structure of C-terminally truncated aIF5B from Pyrococcus abyssi complexed with GTP 手法: X-RAY DIFFRACTION / 解像度: 1.7 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-NO3: NITRATE ION / 硝酸塩 ChemComp-HOH: WATER / 水 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-ZN: Unknown entry ChemComp-MET: METHIONINE / メチオニン / メチオニン ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体*YM / 5'-Guanylyl imidodiphosphate
由来	pyrococcus abyssi ge5 (古細菌) pyrococcus abyssi (古細菌) escherichia coli (大腸菌)
キーワード	TRANSLATION (翻訳 (生物学)) / ribosome (リボソーム) / initiator tRNA / initiation factor / eIF5B / IF2 / Initiation complex (イニシエーション) / translation initiation / small ribosomal subunit / aIF5b