EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for the synergistic neutralization of coxsackievirus B1 by a triple-antibody cocktail.
ジャーナル・号・ページ	Cell Host Microbe, Vol. 30, Issue 9, Page 1279-11294.e6, Year 2022
掲載日	2022年9月14日
著者	Qingbing Zheng / Rui Zhu / Zhichao Yin / Longfa Xu / Hui Sun / Hai Yu / Yuanyuan Wu / Yichao Jiang / Qiongzi Huang / Yang Huang / Dongqing Zhang / Liqin Liu / Hongwei Yang / Maozhou He / Zhenhong Zhou / Yanan Jiang / Zhenqin Chen / Huan Zhao / Yuqiong Que / Zhibo Kong / Lizhi Zhou / Tingting Li / Jun Zhang / Wenxin Luo / Ying Gu / Tong Cheng / Shaowei Li / Ningshao Xia /
PubMed 要旨	Coxsackievirus B1 (CVB1) is an emerging pathogen associated with severe neonatal diseases including aseptic meningitis, myocarditis, and pancreatitis and also with the development of type 1 diabetes. ...Coxsackievirus B1 (CVB1) is an emerging pathogen associated with severe neonatal diseases including aseptic meningitis, myocarditis, and pancreatitis and also with the development of type 1 diabetes. We characterize the binding and therapeutic efficacies of three CVB1-specific neutralizing antibodies (nAbs) identified for their ability to inhibit host receptor engagement. High-resolution cryo-EM structures showed that these antibodies recognize different epitopes but with an overlapping region in the capsid VP2 protein and specifically the highly variable EF loop. Moreover, they perturb capsid-receptor interactions by binding various viral particle forms. Antibody combinations achieve synergetic neutralization via a stepwise capsid transition and virion disruption, indicating dynamic changes in the virion in response to multiple nAbs targeting the receptor-binding site. Furthermore, this three-antibody cocktail protects against lethal challenge in neonatal mice and limits pancreatitis and viral replication in a non-obese diabetic mouse model. These results illustrate the utility of nAbs for rational design of therapeutics against picornaviruses such as CVB.
リンク	Cell Host Microbe / PubMed:36002016
手法	EM (単粒子)
解像度	3.02 - 4.18 Å
構造データ	32967 7x2g EMDB-32967, PDB-7x2g: Cryo-EM structure of Coxsackievirus B1 empty particle in complex with nAb nAb 2E6 (CVB1-E:2E6) 手法: EM (単粒子) / 解像度: 3.58 Å 32968 7x2i EMDB-32968, PDB-7x2i: Cryo-EM structure of Coxsackievirus B1 pre-A particle in complex with nAb 2E6 (CVB1-pre-A:2E6) 手法: EM (単粒子) / 解像度: 3.29 Å 32969 7x2o EMDB-32969, PDB-7x2o: Cryo-EM structure of Coxsackievirus B1 mature virion in complex with nAb 2E6 (CVB1-M:2E6) 手法: EM (単粒子) / 解像度: 3.15 Å 32970 7x2t EMDB-32970, PDB-7x2t: Cryo-EM structure of Coxsackievirus B1 mature virion in complex with nAb 8A10 (CVB1-M:8A10) 手法: EM (単粒子) / 解像度: 3.69 Å 32973 7x2w EMDB-32973, PDB-7x2w: Cryo-EM structure of Coxsackievirus B1 pre-A particle in complex with nAb 8A10 (CVB1-pre-A:8A10) 手法: EM (単粒子) / 解像度: 3.24 Å 32980 7x37 EMDB-32980, PDB-7x37: Cryo-EM structure of Coxsackievirus B1 A particle in complex with nAb 2E6 (CVB1-A:2E6) 手法: EM (単粒子) / 解像度: 3.31 Å 32981 7x38 EMDB-32981, PDB-7x38: Cryo-EM structure of Coxsackievirus B1 empty particle in complex with nAb 8A10 (CVB1-E:8A10) 手法: EM (単粒子) / 解像度: 3.52 Å 32983 7x3c EMDB-32983, PDB-7x3c: Cryo-EM structure of Coxsackievirus B1 muture virion in complex with nAbs 8A10 and 5F5 (CVB1-M:8A10:5F5) 手法: EM (単粒子) / 解像度: 3.03 Å 32984 7x3d EMDB-32984, PDB-7x3d: Cryo-EM structure of Coxsackievirus B1 mature virion in complex with nAb 9A3 (CVB1-M:9A3) 手法: EM (単粒子) / 解像度: 3.48 Å 32985 7x3e EMDB-32985, PDB-7x3e: Cryo-EM structure of Coxsackievirus B1 pre-A-particle in complex with nAb 9A3 (CVB1-pre-A:9A3) 手法: EM (単粒子) / 解像度: 3.44 Å 32986 7x3f EMDB-32986, PDB-7x3f: Cryo-EM structure of Coxsackievirus B1 A-particle in complex with nAb 9A3 (CVB1-A:9A3) 手法: EM (単粒子) / 解像度: 3.52 Å 32998 7x40 EMDB-32998, PDB-7x40: Cryo-EM structure of Coxsackievirus B1 mature virion in complex with nAb 8A10 (classified from CVB1 mature virion in complex with 8A10 and 2E6) 手法: EM (単粒子) / 解像度: 3.02 Å 32999 7x42 EMDB-32999, PDB-7x42: Cryo-EM structure of Coxsackievirus B1 pre-A-particle in complex with nAb 8A10 (classified from CVB1 mature virion in complex with 8A10 and 2E6) 手法: EM (単粒子) / 解像度: 3.88 Å 33000 7x46 EMDB-33000, PDB-7x46: Cryo-EM structure of Coxsackievirus B1 A-particle in complex with nAb 2E6 (classified from CVB1 mature virion in complex with 8A10 and 2E6) 手法: EM (単粒子) / 解像度: 3.85 Å 33001 7x47 EMDB-33001, PDB-7x47: Cryo-EM structure of Coxsackievirus B1 empty particle in complex with nAb 2E6 (classified from CVB1 mature virion in complex with 8A10 and 2E6) 手法: EM (単粒子) / 解像度: 3.66 Å 33002 7x49 EMDB-33002, PDB-7x49: Cryo-EM structure of Coxsackievirus B1 mature virion in complex with nAb 8A10 (classified from CVB1 mature virion in complex with 8A10 and 9A3) 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-33003: Cryo-EM structure of Coxsackievirus B1 A-particle in complex with nAb 9A3 (classified from CVB1 mature virion in complex with 8A10 and 9A3) 手法: EM (単粒子) / 解像度: 4.01 Å 33004 7x4k EMDB-33004, PDB-7x4k: Cryo-EM structure of Coxsackievirus B1 empty particle in complex with nAb 9A3 (classified from CVB1 mature virion in complex with 8A10 and 9A3) 手法: EM (単粒子) / 解像度: 3.82 Å 33005 7x4m EMDB-33005, PDB-7x4m: Cryo-EM structure of Coxsackievirus B1 mature virion in complex with nAb 8A10 (classified from CVB1 mature virion in complex with 8A10, 2E6 and 9A3) 手法: EM (単粒子) / 解像度: 3.34 Å EMDB-33006: Cryo-EM structure of Coxsackievirus B1 empty particle in complex with nAb 2E6 (classified from CVB1 mature virion in complex with 8A10, 2E6 and 9A3) 手法: EM (単粒子) / 解像度: 4.18 Å
化合物	ChemComp-PLM: PALMITIC ACID / パルミチン酸 / パルミチン酸
由来	Severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) mus musculus (ハツカネズミ) coxsackievirus b1 (コクサッキーウイルス)
キーワード	VIRUS (ウイルス) / Coxsackievirus B1 / Neutralizing antiboy / Cryo-EM (低温電子顕微鏡法) / Neutralizing antibody (中和抗体)