EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transport Cycle of Plasma Membrane Flippase ATP11C by Cryo-EM.
ジャーナル・号・ページ	Cell Rep, Vol. 32, Issue 13, Page 108208, Year 2020
掲載日	2020年9月29日
著者	Hanayo Nakanishi / Tomohiro Nishizawa / Katsumori Segawa / Osamu Nureki / Yoshinori Fujiyoshi / Shigekazu Nagata / Kazuhiro Abe /
PubMed 要旨	ATP11C, a plasma membrane phospholipid flippase, maintains the asymmetric distribution of phosphatidylserine accumulated in the inner leaflet. Caspase-dependent inactivation of ATP11C is essential ...ATP11C, a plasma membrane phospholipid flippase, maintains the asymmetric distribution of phosphatidylserine accumulated in the inner leaflet. Caspase-dependent inactivation of ATP11C is essential for an apoptotic "eat me" signal, phosphatidylserine exposure, which prompts phagocytes to engulf cells. We show six cryo-EM structures of ATP11C at 3.0-4.0 Å resolution in five different states of the transport cycle. A structural comparison reveals phosphorylation-driven domain movements coupled with phospholipid binding. Three structures of phospholipid-bound states visualize phospholipid translocation accompanied by the rearrangement of transmembrane helices and an unwound portion at the occlusion site, and thus they detail the basis for head group recognition and the locality of the protein-bound acyl chains in transmembrane grooves. Invariant Lys880 and the surrounding hydrogen-bond network serve as a pivot point for helix bending and precise P domain inclination, which is crucial for dephosphorylation. The structures detail key features of phospholipid translocation by ATP11C, and a common basic mechanism for flippases is emerging.
リンク	Cell Rep / PubMed:32997992
手法	EM (単粒子)
解像度	3.0 - 4.0 Å
構造データ	30163 7bsp EMDB-30163, PDB-7bsp: Cryo-EM structure of a human ATP11C-CDC50A flippase in E1-AMPPCP state 手法: EM (単粒子) / 解像度: 4.0 Å 30164 7bsq EMDB-30164, PDB-7bsq: Cryo-EM structure of a human ATP11C-CDC50A flippase in E1AlF-ADP state 手法: EM (単粒子) / 解像度: 3.2 Å 30165 7bss EMDB-30165, PDB-7bss: Cryo-EM structure of a human ATP11C-CDC50A flippase in E1AlF state 手法: EM (単粒子) / 解像度: 3.3 Å 30167 7bsu EMDB-30167, PDB-7bsu: Cryo-EM structure of a human ATP11C-CDC50A flippase in PtdSer-bound E2BeF state 手法: EM (単粒子) / 解像度: 3.2 Å 30168 7bsv EMDB-30168, PDB-7bsv: Cryo-EM structure of a human ATP11C-CDC50A flippase in PtdSer-occluded E2-AlF state 手法: EM (単粒子) / 解像度: 3.0 Å 30169 7bsw EMDB-30169, PDB-7bsw: Cryo-EM structure of a human ATP11C-CDC50A flippase in PtdEtn-occluded E2-AlF state 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-ACP: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / β,γ-メチレンATP / AMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-MAN: alpha-D-mannopyranose / α-D-マンノピラノ-ス / マンノース ChemComp-P5S: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / O-(1-O,2-O-ジステアロイル-L-グリセロ-3-ホスホ)セリン / ホスファチジルセリン ChemComp-AH2: 1-deoxy-alpha-D-mannopyranose / 1,5-アンヒドロ-D-マンニト-ル ChemComp-HOH: WATER / 水 ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質*YM / Discrete optimized protein energy
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Flippase / P-type ATPase / P4-ATPase / Phospholipid (リン脂質)